Isolation and Characterization of Biosurfactant Produced by Lactic Acid Bacteria from Indigenous Thai Fermented Foods

Abstract

Biosurfactant-producing lactic acid bacteria were isolated from the indigenous Thai fermented foods. MRS-oil agar and surface tension measuring methods were used as primary and secondary screening, respectively. There were 78 LAB isolates from 24 samples of the indigenous Thai fermented foods showed halo zone on MRS-oil agar. PS001 isolate derived from pickled fish (Pla Som) represented the lowest surface tension with 54.33 mN/m of PBS supernatant. PS001 produced cell-bound biosurfactant as growth associated product. For biosurfactant extraction, PBS supernatant was acid precipitated and followed by extraction with a mixture of chloroform:methanol (2:1). Its chemical structure of purified biosurfactant from PS001 was identified by TLC and FTIR spectroscopy as glycoprotein. 

Microorganisms, the Ultimate Tool for Clean Label Foods?

“…However, all these strains are hardly usable as clean label starters in food because of potential hazards or sensorial impact. Fortunately, lactic acid bacteria which are often Qualified Presumption of Safety species used in foods, are also microbes reputed to produce biosurfactants [97]. Biosurfactants derived from Lactococcus lactis showed microbial inhibition against multi-drug resistant pathogens including E. coli and methicillin resistant S. aureus [98].…”

Microbial glycoconjugates in organic pollutant bioremediation: recent advances and applications

“…wt. glycoprotein Electrophoretic analysis [ 84 ] Ochrobactrum anthropi HM-1 Glycolipid Rhamnolipid type TLC and FTIR spectra analysis [ 85 ] Citrobacter freundii HM-2 Glycolipid Rhamnolipid type TLC and FTIR spectra analysis [ 85 ] Lactobacillus Glycoprotein -- TLC and FTIR [ 86 ] Pseudomonas isolate DYNA270 Glycolipid Rhamnolipids Mass spectrometry [ 87 ] Streptomyces nocardiopsis A17, Bacillus subtilis ICA56 Glycerol Biosurfactant TLC and LC-MS [ 88 ] Bacillus psudomycoides BS6 Lipopeptide Fatty acid 3-OH and peptide of five amino acid TLC and FTIR [ 89 ] Bacillus subtilis B20, B. subtilis B30 Glycolipopeptide Surfactin Pedant drop method, Lyophilization [ 29 ] Pseudomonas aeruginosa Glycolipid Rhamnolipid [ 90 ] P. aeruginosa MA01 Glycolipid Monorhamnolipid FTIR, electrospray ioni...…”