Isolation and characterization of biologically active alkaloids from marine actinobacteria Nocardiopsis sp. NCS1

Kannan, K.; Pitchiah, Sivaperumal; Gobalakrishnan, Rajagopal; Swarnakumar, N. S.; Rajendran, R.

doi:10.1016/j.bcab.2014.10.005

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…Previous study shows that the highest scavenging was seen in 100 g/ml in a DPPH radical scavenging assay [40]. In recent years much attention has been devoted to natural antioxidants and their association with health benefits.…”

Section: Discussionmentioning

confidence: 93%

Exploration of Antioxidant Effects of Crude Extract of Mangrove Plant - Avicennia marina

Lakshme

Roy

Sivaperumal

et al. 2021

JPRI

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Introduction: Antioxidants are efficient in the prevention of human diseases. Mangroves are high bioactive compounds with good holistic bioactivities including insecticides. Avicennia marina, a mangrove plant which has its origin in South Africa included in the family Acanthaceae. Aim: To explore the antioxidant potential of methanolic extract of mangrove plants, Avicennia marina. Materials and Methods: The fresh leaves of Avicennia marina were collected from Pichavaram mangrove forest area. The leaves were washed and then shade dried for 2-3weeks and turned into a fine powder. Crude methanolic extract of Avicenna marina was prepared. Total antioxidant activity, DPPH Assay and scavenging activity of hydrogen peroxide were performed and antioxidant potential was assessed using ascorbic acid as standard. Results: The scavenging activity increased with increase in concentration of the extract and thus antioxidant activity was dose dependent. The total antioxidant activity was more close to the ascorbic acid equivalence. Conclusion: This study concludes that the methanolic extract of Avicenna marina is capable of scavenging a wide range of free radicals for which it can be exploited for the treatment of various free radical mediated diseases. It is evident that mangrove species as a collective are rich sources of antioxidants, phenolics and antimicrobial compounds.

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Section: Discussionmentioning

confidence: 93%

Exploration of Antioxidant Effects of Crude Extract of Mangrove Plant - Avicennia marina

Lakshme

Roy

Sivaperumal

et al. 2021

JPRI

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“…Further, the extraction and characterization of metabolites and MTT assay against cancer cell line was done followed by the Kamala et al, [31]. The above-mentioned study was done in the Blue Lab, Saveetha Dental College and Hospital.…”

Section: Methodsmentioning

confidence: 99%

Anticancer Potential from Rhizophora mucronata Plant Leaf Associated Streptomyces Species against the Breast Cancer Cell Line

Kishen

Sivaperumal

Anitha

et al. 2021

JPRI

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Introduction: Rhizophora mucronata is small to a medium-sized evergreen tree growing to a height of about 20 to 25 meters (approximately 66 to 82 feet), on the banks of the river. These mangroves have anticancer, antioxidant, antifungal, and viral activities which enhance their existence. Streptomyces species are the largest genus of Actinobacteria. They are gram-positive and are found in soil, decaying vegetation, and mangrove leaves. The breast cancer cell lines are used to test the anticancer potential. Aim: Aim of the study was to assess the antibacterial activity from Rhizophora mucronata, against breast cancer cell lines. Materials and Methods: Rhizophora mucronata leaf samples were collected and Streptomyces sp was isolated from the mangrove leaf. Further, extraction metabolites from Streptomyces were done and an MTT assay was checked against the cancer cell line. Results and Discussion: The Rhizophora mucronata associated Streptomyces sp was identified by colour of aerial mycelium, soluble pigments, and Spore chain morphology. Further, the secondary metabolites production was done. The potential anticancer activity from the Streptomyces metabolites was done. Conclusion: Rhizophora mucronata mangrove plant leaf associated with Marine Streptomyces showed good anti-cancer activity. Further, an in-vivo study using the metabolites is possible in the future.

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“…Previous reports suggested that Streptomyces was disproportionately present in the waters of the Southern Ocean waters. Streptomyces was also found to be dominant in the Southern Ocean waters, as in the other environmental counterparts[27]. Studies ofLavin et al [28] have also reported the presence of Streptomyces in the Fildes Peninsula soils of King George Island, Antarctica.…”

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confidence: 88%

Exogenous Production of Cold-Active Cellulase From Psychrophilic Actinobacteria With Increased Cellulose Hydrolysis Efficiency

Sivasankar¹,

Poongodi²,

Kannan³

et al. 2021

Preprint

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Actinobacteria form the largest phylum consisting of diverse, ecologically unique and biologically active members. The actinobacteria are omnipresent and occur in various habitats such as cold environment, aquatic, desert and terrestrial ecosystems. Though the studies are available on actinobacteria at various habitats very few reports are available on cold tolerant/loving actinobacteria in the Southern Ocean part of the Antarctic Ocean. In this context, the present work was designed to isolate and characterize the actinobacteria in the Polar Front region of the Southern Ocean waters and species of Nocardiopsis and Streptomyces were identified. Among those, the psychrophilic actinobacterium, Nocardiopsis dassonvillei PSY13 was found to have good cellulolytic activity and it was further studied for the production and characterization of cold-active cellulase enzyme. The latter was found to have a specific activity of 6.36 U/mg and a molar mass of 48 kDa with a 22.9-fold purification and 5% recovery at an optimum pH of 7.5 and a temperature of 10 ºC. Given the importance of psychrophilic actinobacteria N. dassonvillei PSY13 can be further exploited for its benefits, meaning that the Southern Ocean harbours biotechnologically important microorganisms that can be further explored for versatile biotechnological and industrial applications.

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Isolation and characterization of biologically active alkaloids from marine actinobacteria Nocardiopsis sp. NCS1

Cited by 16 publications

References 38 publications

Exploration of Antioxidant Effects of Crude Extract of Mangrove Plant - Avicennia marina

Exploration of Antioxidant Effects of Crude Extract of Mangrove Plant - Avicennia marina

Anticancer Potential from Rhizophora mucronata Plant Leaf Associated Streptomyces Species against the Breast Cancer Cell Line

Exogenous Production of Cold-Active Cellulase From Psychrophilic Actinobacteria With Increased Cellulose Hydrolysis Efficiency

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