Isolation and characterization of bioactive compounds from plant resources: The role of analysis in the ethnopharmacological approach

Brusotti, Gloria; Cesari, I.; Dentamaro, Alessandra; Caccialanza, Gabriele; Massolini, Gabriella

doi:10.1016/j.jpba.2013.03.007

Cited by 226 publications

(124 citation statements)

References 108 publications

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“…Plants represent the most important source of antimicrobial compounds. Their usage in traditional medicine as remedies for several diseases is most popular for 80% of world population in Asia, Africa and South America (Gautam et al, 2012;Brusotti et al, 2013). The M. malabathricum leaves have been reported to possess antibacterial activity (Sunilson et al, 2008;Choudhury et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM <I>MELASTOMA MALABATHRICUM</I> LINN LEAVES

Alwash¹

2013

American Journal of Infectious Diseases

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The present study aimed to assess the antibacterial activity of the methanol extract of Melastoma Malabathricum Leaves (MMML), active fraction of M. malabathricum leaves (ML5) and isolated kaempferol-3-O-(2",6"-di-O-p-trans-coumaroyl)-β-glucopyranoside (Kf) and to describe the preliminary mode of action against Gram-positive and Gram-negative bacteria. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum leaves fractions (ML1-ML6) of increasing polarities. Bioassay-guided fractionation (direct-TLC bioautography) revealed that fraction of M. malabathricum Leaves (ML5) had the highest antibacterial activity. Therefore, the ML5 was further subjected to Column Chromatography (CC) to afford Kaempferol-3-O-(2",6"-di-O-p-trans-coumaroyl)-β-glucopyranoside (Kf). The compound structure was elucidated using data obtained from 1 H NMR and 13 C NMR spectra. Antibacterial activity of MMML, ML5 and bioactive constituents Kf against Staphylococcus aureus reference strain, Methicillin-Resistant S. Aureus reference strain (MRSA), 11 clinical MRSA isolates, 3 clinical Pseudomonas aeruginosa isolates and P. aeruginosa reference strain was evaluated through disk diffusion method and Minimum Inhibitory Concentration (MIC). The time kill assay and field emission scanning electron microscopy was used to determine the preliminary mode of action. The data obtained from disk diffusion method and MIC showed that the MMML, ML5 and Kf showed antibacterial activity against all test bacteria with various values. Generally, the ML5 and Kf exhibited the highest antibacterial activity with the largest zones of inhibition and lowest MIC values against S. aureus and all tested MRSA. The MMML exerted the strongest killing effect against all tested bacteria while the overall performance of ML5 and Kf were significantly more effective against S. aureus and MRSA than P. aeruginosa. Field emission scanning electron microscopy revealed irregular bacterial surface indicating that the MMML, ML5 and Kf disrupted the integrity of membrane. The results obtained showed that MMML, ML5 and Kf have potential alternative to antibiotics.

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Section: Discussionmentioning

confidence: 99%

IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM <I>MELASTOMA MALABATHRICUM</I> LINN LEAVES

Alwash¹

2013

American Journal of Infectious Diseases

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“…Medicinal plants are important therapeutic agents for medicine due to their alternativity to chemical products and most of them have valuable bioactive phytochemicals compounds (Brusotti et al, 2014;Agbor et al, 2005). These phytochemicals have physiological effects on humans due to antioxidant, antibacterial and antifungal activities of flavonoids, terpenoids, vitamins and alkaloids.…”

Section: Introductionmentioning

confidence: 99%

“…These phytochemicals have physiological effects on humans due to antioxidant, antibacterial and antifungal activities of flavonoids, terpenoids, vitamins and alkaloids. Many compounds of the medicinal plants have been isolated and identified as valuable drugs in modern medicine system (Brusotti et al, 2014) and researchers still make an effort on screening of plant extracts to discover new compounds.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Çakır¹,

Pekmez²,

Cepni³

et al. 2014

Food Sci. Technol (Campinas)

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“…64 UV/VIS/FLD spectrometry coupled with chromatography is also a useful technique in bioactive components identification. [65][66][67][68] It is usually coupled with planar chromatography from which images can be captures directly on the plate. Most of these experiments can be measured directly from analytical plates.…”

Section: Structure Identification and Elucidationmentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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Isolation and characterization of bioactive compounds from plant resources: The role of analysis in the ethnopharmacological approach

Cited by 226 publications

References 108 publications

IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM <I>MELASTOMA MALABATHRICUM</I> LINN LEAVES

IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM <I>MELASTOMA MALABATHRICUM</I> LINN LEAVES

Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

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