IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM &lt;I&gt;MELASTOMA MALABATHRICUM&lt;/I&gt; LINN LEAVES

Alwash,

doi:10.3844/ajidsp.2013.46.58

Cited by 24 publications

(23 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar reports on morphological deteriorating effects of several phytochemicals have been observed previously against various foodborne pathogens (Soylu et al, 2007; Alwash et al, 2013). Such morphological and physiological changes could be caused by the changes in the integrity and fluidity of plasma membrane as well as changes in the composition of the lipid profile of plasma membrane directly hampered by the antimicrobial action of treating agent (Sikkema et al, 1994).…”

Section: Discussionsupporting

confidence: 89%

“…Development of natural alternative means as safe antimicrobials is essential in combating serious foodborne pathogens which pose significant threat to humans (Alwash et al, 2013). Among several studied foodborne pathogens, to some extent, Escherichia coli O157:H7, and Staphylococcus aureus are known to be causative agents of foodborne diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Among several studied foodborne pathogens, to some extent, Escherichia coli O157:H7, and Staphylococcus aureus are known to be causative agents of foodborne diseases. The development of resistance of foodborne pathogens in the commercial antibiotics and the emergence of new strains are widespread concerns (Alwash et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antibacterial Action of Jineol Isolated from Scolopendra subspinipes mutilans against Selected Foodborne Pathogens

et al. 2017

View full text Add to dashboard Cite

This study was undertaken to assess the antibacterial potential of 3,8-dihydroxyquinoline (jineol) isolated from Scolopendra subspinipes mutilans against selected foodborne pathogens Escherichia coli O157:H7 and Staphylococcus aureus KCTC-1621. Jineol at the tested concentration (50 μL; corresponding to 250 μg/disk) exhibited significant antibacterial effects as a diameter of inhibition zones (11.6–13.6 mm), along with minimum inhibitory concentration (MIC) and minimum bactericidal concentration values found in the range of (62.5–125 μg/mL) and (125–250 μg/mL), respectively. Jineol also exhibited significant antibacterial effects as confirmed by the reduction in bacterial cell viabilities, increasing release of potassium (K+) ions (650 and 700 mmole/L) and 260 nm materials (optical density: 2.98–3.12) against both the tested pathogens, E. coli O157:H7 and S. aureus KCTC-1621, respectively. Moreover, changes in the cell wall morphology of E. coli O157:H7 and S. aureus KCTC-1621 cells treated with jineol at MIC further confirmed its inhibitory potential against the tested pathogens, suggesting its role as an effective antimicrobial to control foodborne pathogens.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibacterial Action of Jineol Isolated from Scolopendra subspinipes mutilans against Selected Foodborne Pathogens

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Previous studies have elucidated that K F can inhibit P. aeruginosa growth [6,7]. In regard to this inhibitory effect, the approach of transcriptomic analysis is useful to identify the differentially expressed genes in this bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…Strains of P. aeruginosa are aeruginosa infections are increasingly required and gaining more and more attention [4]. Previous studies by our research group demonstrated that K F can induce P. aeruginosa cell wall damage [6,7]. us, we decided to investigate the gene expression profile of P. aeruginosa growing in kaempferol-3-O-(2′,6′-di-O-trans-p-coumaroyl)-β-D glucopyranoside isolated from Melastoma malabathricum known to locals in Malaysia as "senduduk."…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Response inPseudomonas aeruginosatowards Treatment with a Kaempferol Isolated fromMelastoma malabathricumLinn Leaves

Alwash

Aqma

Ahmad

et al. 2020

International Journal of Microbiology

View full text Add to dashboard Cite

Pseudomonas aeruginosa is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients. Kaempferol-3-O-(2′,6′-di-O-trans-p-coumaroyl)-β-D glucopyranoside (KF) is an antipseudomonal compound isolated from the leaves of the native medicinal plant Melastoma malabathricum. Herein, an RNA-seq transcriptomic approach was employed to study the effect of KF treatment on P. aeruginosa and to elucidate the molecular mechanisms underlying the response to KF at two time points (6 h and 24 h incubation). Quantitative real-time PCR (qRT-PCR) was performed for four genes (uvrD, sodM, fumC1, and rpsL) to assess the reliability of the RNA-seq results. The RNA-seq transcriptomic analysis revealed that KF increases the expression of genes involved in the electron transport chain (NADH-I), resulting in the induction of ATP synthesis. Furthermore, KF also increased the expression of genes associated with ATP-binding cassette transporters, flagella, type III secretion system proteins, and DNA replication and repair, which may further influence nutrient uptake, motility, and growth. The results also revealed that KF decreased the expression of a broad range of virulence factors associated with LPS biosynthesis, iron homeostasis, cytotoxic pigment pyocyanin production, and motility and adhesion that are representative of an acute P. aeruginosa infection profile. In addition, P. aeruginosa pathways for amino acid synthesis and membrane lipid composition were modified to adapt to KF treatment. Overall, the present research provides a detailed view of P. aeruginosa adaptation and behaviour in response to KF and highlights the possible therapeutic approach of using plants to combat P. aeruginosa infections.

show abstract

Metabolic profiling, ADME pharmacokinetics, molecular docking studies and antibacterial potential of Phyllantus muellerianus leaves

Obuotor

Kolawole

Apalowo

et al. 2021

ADV TRADIT MED (ADTM)

View full text Add to dashboard Cite

Global increase in the level of antimicrobial resistance among bacterial pathogens has prompted the search for alternative treatment from medicinal plants. Phyllantus muellerianus leaves has been used traditionally against microorganisms of medical importance, hence the need to evaluate the pharmacological pathways and mode of actions using in vitro and in silico approaches. Clinical isolates of eight ( 8) microorganisms associated with urinary tract infections were obtained and identified using morphological and biochemical methods. Phyllantus muellerianus leaves were extracted and purified by solvent partitioning. Ethyl acetate fraction of PM had the highest yield and zone diameter range from 13.5 ± 1.00 to 28 ± 1.53 mm. The rate of protein leakage per time interval of Staphylococcus aureus increased from 9.29 μg/ml at 0 min to 17.43 μg/ml at 120 min while leakage in Candida albicans also increased from 8.57 μg/ml at 0 min to 70.43 μg/ml at 120 min. GCMS fingerprints, pharmacodynamics and pharmacokinetic studies revealed the active agent as quindoline, an azaindole and isotere of indoles having a binding energy of −9.1 kcal/mol. Analyses of the structural and atomic orientations of quindoline, and superimposition on ciprofloxacin, a common antibiotic revealed an interesting comparison, effecting a stronger binding affinity of Quindoline-HMG-CoA complex.

show abstract

IDENTIFICATION AND MODE OF ACTION OF ANTIBACTERIAL COMPONENTS FROM <I>MELASTOMA MALABATHRICUM</I> LINN LEAVES

Cited by 24 publications

References 17 publications

Antibacterial Action of Jineol Isolated from Scolopendra subspinipes mutilans against Selected Foodborne Pathogens

Antibacterial Action of Jineol Isolated from Scolopendra subspinipes mutilans against Selected Foodborne Pathogens

Transcriptomic Response inPseudomonas aeruginosatowards Treatment with a Kaempferol Isolated fromMelastoma malabathricumLinn Leaves

Metabolic profiling, ADME pharmacokinetics, molecular docking studies and antibacterial potential of Phyllantus muellerianus leaves

Contact Info

Product

Resources

About