Transcriptomic Response inPseudomonas aeruginosatowards Treatment with a Kaempferol Isolated fromMelastoma malabathricumLinn Leaves

Abstract

Pseudomonas aeruginosa is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients. Kaempferol-3-O-(2′,6′-di-O-trans-p-coumaroyl)-β-D glucopyranoside (KF) is an antipseudomonal compound isolated from the leaves of the native medicinal plant Melastoma malabathricum. Herein, an RNA-seq transcriptomic approach was employed to study the effect of KF treatment on P. aeruginosa and to elucidate the molecular mechanisms underlying the response to KF at two time points (6 h and 24 h incubation). Quantitative real-time PCR (qRT-PCR) was performed for four genes (uvrD, sodM, fumC1, and rpsL) to assess the reliability of the RNA-seq results. The RNA-seq transcriptomic analysis revealed that KF increases the expression of genes involved in the electron transport chain (NADH-I), resulting in the induction of ATP synthesis. Furthermore, KF also increased the expression of genes associated with ATP-binding cassette transporters, flagella, type III secretion system proteins, and DNA replication and repair, which may further influence nutrient uptake, motility, and growth. The results also revealed that KF decreased the expression of a broad range of virulence factors associated with LPS biosynthesis, iron homeostasis, cytotoxic pigment pyocyanin production, and motility and adhesion that are representative of an acute P. aeruginosa infection profile. In addition, P. aeruginosa pathways for amino acid synthesis and membrane lipid composition were modified to adapt to KF treatment. Overall, the present research provides a detailed view of P. aeruginosa adaptation and behaviour in response to KF and highlights the possible therapeutic approach of using plants to combat P. aeruginosa infections.

Analysis of Antiquorum-Sensing and Antibiofilm Activity by Pomelo Peel Extract (Citrus maxima) on Multidrug-Resistance Pseudomonas aeruginosa

“…Kaempferol could decrease the expression of genes involved in biofilm formation in P. aeruginosa . The type IV and flagellin type B (fliC) genes’ downregulation in response to kaempferol observed in RNA-seq was paralleled by the decreased expression of genes which are involved in biofilm formation in P. aeruginosa [ 33 ]. Rutin, which is also found in Citrus maxima , showed a concentration-dependent reduction of biofilm formation.…”

Suppressing Alpha-Hemolysin as Potential Target to Screen of Flavonoids to Combat Bacterial Coinfection

“…Moreover, when counting the number of lung colony in the coinfection mice model, it was found that both kaempferol treatment and prevention group could reduce the number of P. aeruginosa in lung tissue, indicating that kaempferol could effectively inhibit the growth of P. aeruginosa in vivo and in vitro. Mourouge et al found that Kaempferol-3o-(2 ,6 -di-o-trans-p-coumaryl)-β-d-glucopyranoside (KF) isolated from Melastomataceae, which can inhibit P. aeruginosa by inhibiting the expression of virulence factors, affects its nutrient absorption, movement, and growth mechanism when they studied the use of plants to treat acute P. aeruginosa infections, indicating that kaempferol has great potential in the inhibition of P. aeruginosa [38].…”