Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source

Abstract

Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Pseudomonas mendocina (ALS1131), and Pseudomonas putida (ALS1267). In all cases, growth under oxygenated conditions on furfural was accompanied by the transient accumulation of 2-furoic acid (furoate) with no furfuryl alcohol observed. ALS1267 and ALS1279 were also able to metabolize 5-(hydroxymethyl)furfural. The five isolates and their phylogenetic near neighbors were compared for furfural dehydrogenase activity and tolerance to furfural and furoate in defined and complex media. P. putida ALS1267 was the most tolerant to furans and tolerated 17 mM furfural or 195 mM furoate before its growth rate was reduced by 50 % in a defined medium. This strain also had the greatest specific growth rate on furfural (0.6/h at 27-30 °C) and showed the highest specific activity of furfural dehydrogenase (170 mIU/mg) of any furfural-utilizing strain that has been characterized to date.

Progressive deterioration of the upper respiratory tract and the gut microbiomes in children during the early infection stages of COVID-19

“…The pathway “Biofilm formation- Pseudomonas aeruginosa ” was significantly enriched in both COVID-TN-II and COVID-TN-III. Furfural can be used as carbon sources of Pseudomonas ( Lee et al, 2016 ). Furfural degradation pathway was enriched in COVID-TN-III.…”

Widespread distribution of hmf genes in Proteobacteria reveals key enzymes for 5-hydroxymethylfurfural conversion

“…1 ). We also include the genomes of three additional Pseudomonas species (ALS1131, ALS1267 and ALS1279) with verified furans-degrading abilities [43] , [44] collecting a total of 101 strains. Nevertheless, two bacterial species harboring HmfA, representing Inquilinus (GCA_002195995.1) and Acidisphaera (GCA_000964365.1) genus of the α-Proteobacteria lineage (order Rhodospirillales ) were subsequently discarded due to low quality genome assembly.…”

Bioupgrading of the aqueous phase of pyrolysis oil from lignocellulosic biomass: a platform for renewable chemicals and fuels from the whole fraction of biomass

“…The first strategy is to isolate and select some microorganisms that can grow on the pyrolytic aqueous phase and tolerate toxic components. Lee et al ( 2016 ) isolated some bacteria that can utilize furfural from wastewater treatment plants. In another study, Lee et al ( 2010 ) found that Corynebacterium glutamicum ATCC 13032 could decompose phenol of 0.8 g/L and the presence of phenol in the culture medium enhanced the production of glutamate and proline.…”