Isolation and Characterization of Antimicrobial Compounds in Plant Extracts against Multidrug-Resistant Acinetobacter baumannii

Miyasaki, Yoko; Rabenstein, John; Rhea, Joshua; Crouch, Marie-Laure; Mocek, Ursula; Kittell, Patricia Emmett; Morgan, Margie; Nichols, W. Stephen; Benschoten, M. M. Van; Hardy, William; Liu, George Y.

doi:10.1371/journal.pone.0061594

Cited by 98 publications

(76 citation statements)

References 22 publications

(32 reference statements)

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“…The predominant feature of the inhibition curve shown by corilagin is that it is biphasic in nature. This study identified that the compound norwogonin was the highly effective compound against A. baumanii (Miyasaki et al, 2013). The 50% aqueous ethanolic extract of Acalypha wilkesiana and Acalypha hispida leaves showed good antimicrobial effect against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Candida pseudotropicalis (Adesina et al, 2000).…”

Section: Antibacterial Activitymentioning

confidence: 95%

Scope of Hydrolysable Tannins as Possible Antimicrobial Agent

2016

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Hydrolysable tannins (HTs) are secondary metabolites from plants, which are roughly classified into gallotannins and ellagitannins having gallic acid and ellagic acid residues respectively attached to the hydroxyl group of glucose by ester linkage. The presence of hexahydroxydiphenoyl and nonahydroxyterphenoyl moieties is considered to render antimicrobial property to HTs. HTs also show considerable synergy with antibiotics. Nevertheless, they have low pharmacokinetic property. The present review presents the scope of HTs as future antimicrobial agent. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Antibacterial Activitymentioning

confidence: 95%

Scope of Hydrolysable Tannins as Possible Antimicrobial Agent

2016

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“…41,45,46 Ellagitannins have also been reported to function via several antibiotic mechanisms including interaction with cytoplasmic oxidoreductases and by disrupting bacterial cell walls. 41,45 Interestingly, the benzopyran tannin chebulagic acid (Figure 8h) has been reported to inhibit a multi-resistant strain of Acinetobacter baumannii, 47 a species taxonomically related to A. baylyi, examined in our study.…”

Section: 20mentioning

confidence: 64%

Tannin components and inhibitory activity of Kakadu plum leaf extracts against microbial triggers of autoimmune inflammatory diseases

Courtney¹,

Sirdaarta²,

Matthews³

et al. 2014

Phcog J

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Introduction: Autoimmune inflammatory diseases can be triggered by specific bacteria in susceptible individuals. Terminalia ferdinandiana (Kakadu plum) has documented therapeutic properties as a general antiseptic agent. However, the high ascorbic acid levels in Kakadu plum fruit may interfere with this activity. Methods: T. ferdinandiana leaf solvent extracts were investigated by disc diffusion assay against a panel of bacteria known to trigger autoimmune inflammatory diseases.Their MIC values were determined to quantify and compare their efficacies.Toxicity was determined using the Artemia franciscana nauplii bioassay. Non-targeted HPLC separation of crude extracts coupled to high resolution time-of-flight (TOF) mass spectroscopy with screening against 3 compound databases was used for the identification and characterisation of individual components in crude plant extracts. Results: Methanolic, aqueous and ethyl acetate T. Ferdinandiana leaf extracts displayed potent antibacterial activity in the disc diffusion assay against the bacterial triggers of rheumatoid arthritis, ankylosing spondylitis and multiple sclerosis. The ethyl acetate extract had the most potent inhibitory activity, with MIC values less than 120 µg/ml against P. mirabilis and A. baylyi (both reference and clinical strains). The ethyl acetate extract had similar potency against K. pneumonia (both reference and clinical strains), but had higher MIC values (2733 µg/ml) against P. aeruginosa. The methanolic extract was also a potent inhibitor of bacterial growth, with MIC values generally < 1000 µg/ml. In comparison, the water, chloroform and hexane leaf extracts were all substantially less potent antibacterial agents, with MICs values generally well over 1000 µg/ml. All T. ferdinandiana leaf extracts were either nontoxic or of low toxicity in the Artemia fransiscana bioassay.Non-biased phytochemical analysis of the ethyl acetate extract revealed the presence of high levels of tannins (exifone (4-galloylpyrogallol), ellagic acid dehydrate, trimethylellagic acid, chebulic acid, corilagin, punicalin, castalagin and chebulagic acid). Conclusion: The low toxicity of the T. ferdinandiana leaf extracts and their potent inhibitory bioactivity against the bacterial triggers of autoimmune inflammatory disorders indicates their potential as medicinal agents in the treatment and prevention of these diseases.

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“…The time-kill kinetic index of isolated compound was determined as described by Miyasaki et al [26] with slight modifications. The objective of this test is to know the duration of bactericidal or fungicidal effect of isolated molecule.…”

Section: Methodsmentioning

confidence: 99%

Acthaside: a new chromone derivative from Acacia ataxacantha and its biological activities

Amoussa¹,

Bourjot²,

Lagnika³

et al. 2016

BMC Complement Altern Med

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Background Acacia ataxacantha (Fabaceae), used in traditional medicine grows in the South-West of Bénin. Ethyl acetate extract of the barks of this species was previously reported to display various bioactivities, including antibacterial, antifungal and antioxidant activities. In the present study, we investigate the antimicrobial and antioxidant activities of compound isolated from ethyl acetate extract of Acacia ataxacantha.MethodsPurification, isolation and structural identification of isolated compound were done using various chromatographic and spectroscopic methods. Antimicrobial activity was investigated using a two-fold serial microdilution method. The inhibitory potency of isolated compound was evaluated by kinetic experiments. The antioxidant activity was also determined using 2, 2-diphenyl-1-picrylhydrazyl.ResultsThe isolated compound was identified as 7-hydroxy-2-methyl-6-[β-galactopyranosyl-propyl]-4H-chromen-4-one. As far as we know, this compound, named “acthaside”, reported for the first time, was active against all tested microorganisms with minimal inhibitory concentration ranging from 25 to 50 μg/ml. At 50 μl/ml, no growth was observed in almost all tested microbial after 24 h of exposure. The isolated compound had significant antioxidant activity with an IC50 value of 3.61 ± 0.12 μg/ml compared to quercetin (IC50 1.04 ± 0.01 μg/ml).ConclusionThe present work demonstrates that the new chromen derivative isolated from A. ataxacantha may help treat bacterial and yeast infections. However, further studies are required to clarify the mechanism of action of this compound.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1489-y) contains supplementary material, which is available to authorized users.

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Isolation and Characterization of Antimicrobial Compounds in Plant Extracts against Multidrug-Resistant Acinetobacter baumannii

Cited by 98 publications

References 22 publications

Scope of Hydrolysable Tannins as Possible Antimicrobial Agent

Scope of Hydrolysable Tannins as Possible Antimicrobial Agent

Tannin components and inhibitory activity of Kakadu plum leaf extracts against microbial triggers of autoimmune inflammatory diseases

Acthaside: a new chromone derivative from Acacia ataxacantha and its biological activities

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