Isolation and Characterization of Antibacterial Compound from a Mangrove-Endophytic Fungus, Penicillium chrysogenum MTCC 5108

Abstract

Microorganisms, especially endophytic fungi that reside in the tissue of living mangrove plants, seem to play a major role in meeting the general demand for new biologically active substances. During the course of screening for biologically active secondary metabolites from marine microorganisms, an antibiotic compound containing an indole and a diketopiperazine moiety was isolated from the culture medium of Penicillium chrysogenum, (MTCC 5108), an endophytic fungus on the mangrove plant Porteresia coarctata (Roxb.). The cell free culture medium of P. chrysogenum showed significant activity against Vibrio cholerae, (MCM B-322), a pathogen causing cholera in humans. Bioassay guided chemical characterization of the crude extract led to the isolation of a secondary metabolite possessing a molecular formula C 19 H 21 O 2 N 3 . Its antibacterial activity was comparable with standard antibiotic, streptomycin. This compound (1) was found to be (3,1 0 -didehydro-3[2 00 (3 000 ,3 000 -dimethyl-prop-2-enyl)-3 00 -indolylmethylene]-6-methyl pipera-zine-2,5-dione) on the basis of mass spectrometry, infrared spectroscopy and one and two-dimensional nuclear magnetic resonance analysis.

Antimicrobial Drug Discovery: Evident Shifting from Terrestrial to Marine Micro-organisms

“…The cell free culture medium of P. chrysogenum showed significant activity against Vibrio cholerae, (MCM B-322), a pathogen causing cholera in humans. Its antibacterial activity was comparable with standard antibiotic, streptomycin (Devi et al, 2012).…”

Endophytic Fungi as a Source of Novel Metabolites

“…The crude extract of P. chrysogenum had been active against Vibrio cholerae MCM B-322, a pathogen causing cholera in humans. Then, the antibacterial activity of the pure compound against this pathogen was also tested, indicating that it is comparable to that of the standard antibiotic streptomycin [79].…”

Isolation and Identification of Antibacterial Compounds Isolated from Endophytic Fungus Emericella qaudrilineata

“…Benzyl benzoates and its derivatives (benzyl benzoate, benzyl cinnamate and meta-methyl and 3-methylbenzyl 2-nitrobenzoate) also reduce hypertension [18]. Based on described work a number of chemical characterizations of bioactive compounds have been reported from different endophytic fungi as Penicillium chrysogenum [19], volatile organic compound from Muscodar Albus [20], and javanicin napthaquinone from choridium [21].…”