Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers

Abstract

The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid-base equilibrium (pK'a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.

Novel colorimetric films based on starch/polyvinyl alcohol incorporated with roselle anthocyanins for fish freshness monitoring

“…Different anthocyanins generally demonstrate different color response to pH variation (Garber, Odendaal, & Carlson, 2013). Herein, the color response of the crude RACNs extract solution was an integrated color response of several kind of anthocyanins, mainly including delphinidin-3-sambubioside and cyanidin-3-O-sambubioside (Grajeda-Iglesias, et al, 2016). 3.2.…”

Application of green synthesized nanocrystalline CuI in the removal of aqueous Mn(VII) and Cr(VI) ions

“…Recently, many researchers have attempted to synthesize copper iodide via green and ecologically friendly routes. These attempts comprise the utilization of plant or fruit extracts containing anthocyanins [31] as reducing agents, stabilizer and capping agent [32]. The present study aims at the synthesis of CuI NP using the Hibiscus rosa-sinensis L. flower extract and its use in the removal of aqueous Cr(VI) and Mn(VII) metal ions.…”

Comparison of Standard Elution and Displacement Modes in Centrifugal Partition Chromatography for an Efficient Purification of Four Anthocyanins from Hibiscus sabdariffa L.

“…Compounds 1 and 2 were identified as delphinidin derivatives based on significant fragment masses of the aglycone at m/z 303. Compounds 3 and 4 were identified as cyanidin derivatives based on significant masses of the aglycone at m/z 287 [34][35][36].…”