Isolation and Characterization of an Immortal Neoplastic Cell Line (KS Y-1) From AIDS-Associated Kaposi's Sarcoma

Abstract

KS Y-1 cells in the in vivo mouse model can be used to study the effects of therapeutic compounds in advanced KS.

Activity of the Vascular Targeting Agent Combretastatin A-4 Disodium Phosphate in a Xenograft Model of AIDS-Associated Kaposi's Sarcoma

“…In our study we used the KSY-1 cell line as characterized by Lunardi-Iskander et al (24). This cell line was derived from mononuclear cells from pleural effusion of an AIDS patient with KS, following selective removal of lymphocytes, monocytes:macrophages and broblasts.…”

“…While there are reports of cells derived from Kaposi's sarcoma being propagated in vitro (20)(21)(22), the tumorigenic potential of these cell lines in nude mice generally has been limited (20,23). To carry out the current studies, the KSY-1 cell line, established from a patient with HIV-1 associated KS (24), which readily induces tumors in athymic nude mice was used.…”

The insulin receptor is essential for virus-induced tumorigenesis of Kaposi's sarcoma

“…KS lesions are mainly composed of interweaving bands of vascularizing spindle cells that are of endothelial origin and frequently associated with infiltrating inflammatory cells (Boshoff et al, 1995). Although most spindle cells harbor latent virus (Staskus et al, 1997), KS-derived spindle cells rapidly undergo senescence in cell culture, and the few KS-derived cell lines, such as KSY1 or KSIMM, are highly altered in their karyotypes and do not contain virus (Lunardi-Iskandar et al, 1995;Flamand et al, 1996;Albini et al, 1997). KSHV is unable to immortalize endothelial cells (ECs) in vitro.…”

Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities

“…8 KS Y-1 cells are a Kaposi's sarcoma-derived cell line. 24 The reported values of IC 50 were 6 mg/ml and >100 mg/ml for (À4)EDN and EDN, respectively. Human pancreatic type RNases are generally not cytotoxic to cultured cells, but are as potent as toxins when injected into cells directly.…”

“…Binding assay KS Y-1 cells, Kaposi's sarcoma-derived neoplastic endothelial cells, 24 were plated at 20,000 cells per well of a 48-well plate two days before treatment. Before use, the cells were rinsed and 100 ml of fresh medium added.…”