Isolation and Characterization of a Pentasaccharide from <i>Stellaria media</i>

Vanhaecke, Mieke; Ende, Wim Van den; Lescrinier, Eveline; Dyubankova, Natalia

doi:10.1021/np800274k

Cited by 24 publications

(11 citation statements)

References 36 publications

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“…Bruker Topspin 2.1 software was used to process all spectra. The two-dimensional pulse programs of 1 H- 1 H correlation spectroscopy (COSY), heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC), and nuclear Overhauser effect spectroscopy experiments were carried out as described previously (Vanhaecke et al ., 2008). …”

Section: Methodsmentioning

confidence: 99%

Sedoheptulose accumulation under CO2 enrichment in leaves of Kalanchoë pinnata: a novel mechanism to enhance C and P homeostasis?

Ceusters

Godts

Peshev

et al. 2013

Journal of Experimental Botany

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In contrast to the well-documented roles of its mono- and bisphosphate esters, the occurrence of free sedoheptulose in plant tissues remains a matter of conjecture. The present work sought to determine the origin of sedoheptulose formation in planta, as well as its physiological importance. Elevated CO2 and sucrose induction experiments were used to study sedoheptulose metabolism in the Crassulacean acid metabolism (CAM) plants Kalanchoë pinnata and Sedum spectabile. Experimental evidence suggested that sedoheptulose is produced from the oxidative pentose phosphate pathway intermediate sedoheptulose-7-phosphate, by a sedoheptulose-7-phosphate phosphatase. Carbon flux through this pathway was stimulated by increased triose-phosphate levels (elevated CO2, compromised sink availability, and sucrose incubation of source leaves) and attenuated by ADP and inorganic phosphate (Pi). The accumulation of free sedoheptulose is proposed to act as a mechanism contributing to both C and P homeostasis by serving as an alternative carbon store under elevated CO2 or a compromised sink capacity to avoid sucrose accumulation, depletion of inorganic phosphate, and suppression of photosynthesis. It remains to be established whether this acclimation-avoiding mechanism is confined to CAM plants, which might be especially vulnerable to Pi imbalances, or whether some C3 and C4 plants also dispose of the genetic capacity to induce and accelerate sedoheptulose synthesis upon CO2 elevation.

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Section: Methodsmentioning

confidence: 99%

Sedoheptulose accumulation under CO2 enrichment in leaves of Kalanchoë pinnata: a novel mechanism to enhance C and P homeostasis?

Ceusters

Godts

Peshev

et al. 2013

Journal of Experimental Botany

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“…Intriguingly, Sta does not occur within the Caryophyllaceae. Instead, Raf is elongated to the DP4 lychnose (Galα1,6Glcα1,2βFru1,1Gal) and the DP5 stellariose ([6Galα1,6&Galα1,4]Glcα1,2βFru1,1Gal) in cold-treated Stellaria media (Vanhaecke et al, 2006, 2008, 2010). …”

Section: Introductionmentioning

confidence: 99%

Multifunctional fructans and raffinose family oligosaccharides

Ende¹

2013

Front. Plant Sci.

192

116

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Fructans and raffinose family oligosaccharides (RFOs) are the two most important classes of water-soluble carbohydrates in plants. Recent progress is summarized on their metabolism (and regulation) and on their functions in plants and in food (prebiotics, antioxidants). Interest has shifted from the classic inulin-type fructans to more complex fructans. Similarly, alternative RFOs were discovered next to the classic RFOs. Considerable progress has been made in the understanding of structure–function relationships among different kinds of plant fructan metabolizing enzymes. This helps to understand their evolution from (invertase) ancestors, and the evolution and role of so-called “defective invertases.” Both fructans and RFOs can act as reserve carbohydrates, membrane stabilizers and stress tolerance mediators. Fructan metabolism can also play a role in osmoregulation (e.g., flower opening) and source–sink relationships. Here, two novel emerging roles are highlighted. First, fructans and RFOs may contribute to overall cellular reactive oxygen species (ROS) homeostasis by specific ROS scavenging processes in the vicinity of organellar membranes (e.g., vacuole, chloroplasts). Second, it is hypothesized that small fructans and RFOs act as phloem-mobile signaling compounds under stress. It is speculated that such underlying antioxidant and oligosaccharide signaling mechanisms contribute to disease prevention in plants as well as in animals and in humans.

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“…The species is medicinal and edible, rich in vitamins, minerals, flavonoids, triterpenoids, Gamma-linolenic-acid, phenols and beta carotene. Various phytoconstituents viz ; lipids [7], pentasaccharide [8], and triterpenoid [9] have been reported from this species. It has been used to treat various diseases because of many biological activities in traditional medicine, such as inflammations of the digestive, renal, respiratory and reproductive tracts.…”

Section: Introductionmentioning

confidence: 99%

Quality assessment and anti-obesity activity of Stellaria media (Linn.) Vill

Rani

Vasudeva

Sharma

2012

BMC Complement Altern Med

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BackgroundObesity is recognized as a social problem, associated with serious health risks and increased mortality. Numerous trials have been conducted to find and develop new anti-obesity drugs through herbal sources to minimize side effects associated with the present anti-obesity drugs. The present study was designed to evaluate the quality control parameters, quantitative phytochemical analysis (total phenolic, total flavonoids and total saponin content), and the anti-obesity effect of lyophilized juice (LJ) of Stellaria media (Linn.) Vill. by employing in vitro and in vivo models.MethodsIn vitro studies were performed to evaluate the inhibitory activity of LJ on pancreatic amylase and lipase. The in vivo pancreatic lipase activity was evaluated by measurement of plasma triacylglycerol levels after oral administration of lipid emulsion to swiss albino mice. Furthermore, the anti-obesity effect of LJ was assessed at two doses, 400 mg/kg and 900 mg/kg body weight in mice fed a high-fat-diet with or without LJ for 6 weeks.ResultsThe LJ inhibited pancreatic amylase and lipase activity in vitro and elevated plasma triacylglycerol level in mice. LJ suppressed the increase in body weight, retroperitoneal adipose tissue, liver weights and serum parameters viz., total cholesterol, total triglyceride, LDL-cholesterol level at the dose of 900 mg/kg body weight of the mice fed with high fat diet. The total phenolic, flavonoid and saponin contents were found to be 0.26 mg/g, 1.4 mg/g and 1.19 μg/g respectively of LJ.ConclusionThe anti-obesity effects of LJ in high-fat-diet fed mice may be partly mediated through delaying the intestinal absorption of dietary fat and carbohydrate by inhibiting digestive enzymes.

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Isolation and Characterization of a Pentasaccharide from Stellaria media

Cited by 24 publications

References 36 publications

Sedoheptulose accumulation under CO2 enrichment in leaves of Kalanchoë pinnata: a novel mechanism to enhance C and P homeostasis?

Sedoheptulose accumulation under CO2 enrichment in leaves of Kalanchoë pinnata: a novel mechanism to enhance C and P homeostasis?

Multifunctional fructans and raffinose family oligosaccharides

Quality assessment and anti-obesity activity of Stellaria media (Linn.) Vill

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