BackgroundObesity is recognized as a social problem, associated with serious health risks and increased mortality. Numerous trials have been conducted to find and develop new anti-obesity drugs through herbal sources to minimize side effects associated with the present anti-obesity drugs. The present study was designed to evaluate the quality control parameters, quantitative phytochemical analysis (total phenolic, total flavonoids and total saponin content), and the anti-obesity effect of lyophilized juice (LJ) of Stellaria media (Linn.) Vill. by employing in vitro and in vivo models.MethodsIn vitro studies were performed to evaluate the inhibitory activity of LJ on pancreatic amylase and lipase. The in vivo pancreatic lipase activity was evaluated by measurement of plasma triacylglycerol levels after oral administration of lipid emulsion to swiss albino mice. Furthermore, the anti-obesity effect of LJ was assessed at two doses, 400 mg/kg and 900 mg/kg body weight in mice fed a high-fat-diet with or without LJ for 6 weeks.ResultsThe LJ inhibited pancreatic amylase and lipase activity in vitro and elevated plasma triacylglycerol level in mice. LJ suppressed the increase in body weight, retroperitoneal adipose tissue, liver weights and serum parameters viz., total cholesterol, total triglyceride, LDL-cholesterol level at the dose of 900 mg/kg body weight of the mice fed with high fat diet. The total phenolic, flavonoid and saponin contents were found to be 0.26 mg/g, 1.4 mg/g and 1.19 μg/g respectively of LJ.ConclusionThe anti-obesity effects of LJ in high-fat-diet fed mice may be partly mediated through delaying the intestinal absorption of dietary fat and carbohydrate by inhibiting digestive enzymes.
The present study was designed to evaluate the quality control parameters, quantitative phytochemical analysis (total phenols, total flavonoids, and total saponin content), and the antiobesity effect of ethanol extract of Achyranthes aspera Linn. seed (EAA) by employing in vitro and in vivo models. In in vitro study, the inhibitory activity of EAA on pancreatic amylase and lipase was measured. The in vivo pancreatic lipase activity was evaluated by measurement of plasma triacylglycerol levels after oral administration of EAA along with lipid emulsion to Swiss albino mice. The EAA inhibited pancreatic amylase and lipase activity in vitro and elevations of plasma triacylglycerol level in mice. Furthermore, the antiobesity effect of EAA (900 mg/kg) was assessed in mice fed a high-fat diet with or without EAA for 6 weeks. EAA significantly suppressed the increase in body, retroperitoneal adipose tissue, liver weights, and serum parameters, namely; total cholesterol, total triglyceride, and LDL-cholesterol level. The anti obesity effects of EAA in high-fat-diet-treated mice may be partly mediated through delaying the intestinal absorption of dietary fat by inhibiting pancreatic amylase and lipase activity. Histopathological effects of EAA on the liver of mice were also assessed.
Leucas diffusa (lamiaceae) a branched herb collected from the Guntur region, A.P. India. Phytochemical studies of Leucas diffusa, methanolic fraction subjected to column chromatography and estimation of total flavanoid content and to evaluate antioxidant potential and anti-ulcer activity of methanolic extract of Leucas diffusa. The plant powder was subjected to continuous hot extraction in Soxhlet Apparatus & extracted successively with n-hexane, ethyl acetate and methanol solvents. The extracts prepared were tested for the type of chemical constituents present by known qualitative tests. Total flavanoid content of methanolic and ethylacetate extract of Leucas diffusa were done. In vitro Antioxidant activity of methanolic extract was evaluated using hydroxyl radical, reducing power & hydrogen peroxide scavenging abilities. Anti-ulcer activitiy of methanolic extract was evaluated through pylorus ligation method and ethanol induced ulcer models. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the tukey’s test for multiple comparisons. Our findings suggest that methanolic extract of whole plant Leucas diffusa have got profound antioxidant effect and showed significant anti-ulcer activity at higher dose. Keywords: Leucas diffusa; Antioxidant; Flavanoid; anti-ulcer activity.
Objective:To investigate the anti-obesity potential of the mucilage of the Mimosa pudica Linn. seed on mice fed on a high-fat diet. Materials and Methods: Two in vivo, that is, lipid emulsion and high-fat diet models were used to investigate the antiobesity effect in male Swiss albino mice. In the high-fat diet model, the mice were fed a high-fat diet, containing 60% fat, with or without mucilage, for six weeks. The time course of body weight, food intake, organ and fat pad weight (liver and parametrial fat), and serum parameters (triglyceride, total cholesterol, etc.) were measured. In the other model, lipid emulsion with or without mucilage was administered orally, and the plasma triacylglycerol level was measured at 0 to 5 hours. Results: No significant reduction in body weight, organ or fat pad weight (liver and parametrial fat) or serum parameters (triglyceride, total cholesterol) after treatment with mucilage in high-fat diet fed mice was observed. Furthermore the mucilage did not inhibit the elevation of the plasma triacylglycerol level at five hours after the oral administration of lipid emulsion. Conclusion: The present study indicated that the mucilage of the Mimosa pudica seed did not reveal significant anti-obesity action in high-fat diet fed mice.How to cite this article: Rani N, Sharma SK, Vasudeva N. Lack of antiobesity effect of Mimosa pudica seed mucilage on mice fed on a high-fat diet. J Pharm Negative Results 2012;3:38-40.
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