Isolation and characterization of aSphingomonassp. strain F-7 degrading fenvalerate and its use in bioremediation of contaminated soil

Abstract

A fenvalerate-degrading bacterial strain F-7 was isolated from long-term contaminated sludge. Based on morphological, physiological and biochemical characterization, and phylogenetic analysis of 16S rRNA gene sequence, strain F-7 was identified as Sphingomonas sp. The bacterium could utilize fenvalerate as the sole source of carbon. An amount measuring 100 mg L(-1) fenvalerate was completely degraded within 72 h and 3-phenoxybenzoic acid (3-PBA) was detected as a major metabolite. The result indicates that S. sp. F-7 might metabolize fenvalerate by hydrolysis of carboxylester linkage. It was capable of degrading permethrin, fenpropathrin, beta-cypermethrin, cyhalothrin, deltamethrin, bifenthrin and 3-PBA. Further studies demonstrated that the strain was multi-resistant to heavy metals and antibiotics. In addition, degradative enzymes involved were confirmed as intracellular distributed and constitutively expressed. Furthermore, application of the strain was found to accelerate the removal of fenvalerate in soil. This is the first report of fenvalerate degrading strain isolated from S. sp. These results might help with future research in better understanding of pyrethroid biodegradation and highlight S. sp. F-7 might have potential for practical application in bioremediation of fenvalerate-contaminated sites.

Isolation, identification, and fenvalerate-degrading potential of Bacillus licheniformis CY-012

“…The extracts were analyzed at an injection temperature of 250 C and EI source temperature of 280 C. The extract volume was 1 mL, and the sample was injected into the column with a temperature program of 90 C (2 min), 6 C min ¡1 to 150 C (1 min), 10 C min ¡1 to 180 C (4 min), and 20 C min ¡1 to 260 C (10 min). Metabolites were identified and quantified based on the retention times and peak areas of the pure standards [16].…”

“…However, little information is available concerning the biodegradation of fenvalerate [9,15] and its degradation pathway. Yu et al [16] reported that Sphingomonas sp. F-7 was capable of completely degrading 100 mg L ¡1 fenvalerate within 72 h and 3-phenoxybenzoic acid (3-PBA) was detected as a major metabolite.…”

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

“…At the end of experiment, no metabolite was detected by GC/MS indicating that the metabolite compounds could be further transformed and metabolized. Similar biodegradation pathways have been reported for other pyrethroids such as cypermethrin [14,28], cyfluthrin [9,23], and fenvalerate [15,33]. It could be inferred that ester hydrolysis by carboxylesterases may be the major biodegradation pathway of pyrethroids in microorganism.…”

Biodegradation of Organophosphate and Pyrethroid Pesticides by Microorganims

“…Whereas, 44.4 mg kg −1 of fenvalerate was removed from fresh soil inoculated with strain F-7. It is noteworthy that fenvalerate degradation was faster in nonsterilized soils than in sterilized soils indicating a contribution of indigenous flora to fenvalerate removal (Yu et al 2013).…”