Isolation and characterization of a germ cell marker in teleost fish Colossoma macropomum

Vasconcelos, Ana Carina Nogueira; Streit, Danilo Pedro; Octavera, Anna; Miwa, Misako; Kabeya, Naoki; Garcia, Raycon Roberto Freitas; Rotili, Daniel Antonio; Yoshizaki, Goro

doi:10.1016/j.gene.2018.10.027

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…Both of these genes are considered gonad-specific transcripts (e.g., Fig. 1); gsdf, again being expressed in Sertoli cells and associated with germ cell proliferation (Sawatari et al, 2007;Zhang et al, 2016) and vasa being a universal germ cell marker (Hartung et al, 2014;Vasconcelos et al, 2019). These data together suggest that gsdf may play a role in spermatogonial proliferation in sablefish, later observed via histology and reflected by increases in testis vasa mRNA.…”

Section: Discussionmentioning

confidence: 86%

Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination

Hayman

Fairgrieve

Luckenbach

2021

Gene

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Section: Discussionmentioning

confidence: 86%

Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination

Hayman

Fairgrieve

Luckenbach

2021

Gene

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“…In teleosts, vasa is a useful marker for exploring gonad development and early gametogenesis. It has been identified as a reproductive marker in Asian seabass ( Lates calcarifer ), tambaqui ( Colossoma macropomum ) and rare minnow ( Gobiocypris rarus ) [ 61 , 65 , 68 ]. Additionally, the chimera mRNA of vasa -3′UTR can trace PGCs in zebrafish, rainbow trout ( Oncorhynchus mykiss ), Nibe croaker ( Nibea mitsukurii ), tongue sole ( Cynoglossus semilaevis ) among others [ 69 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (Plectropomus leopardus)

Wang

Ding

et al. 2022

Genes

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Vasa (Ddx4, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, vasa homolog termed Plvasa was isolated and identified Plvasa as a putative germ cell marker in the leopard coral grouper, (Plectropomus leopardus). Results indicated that Plvasa contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that Plvasa was homologous with other teleosts. Additionally, the expression of Plvasa was significantly higher in gonads than in other tissues in adult individuals (p < 0.05). Further, the distribution of Plvasa revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the Plvasa gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.

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“…Oct4, the octamer-binding transcription factor 4 encoded by the gene POU5F1 [40,41], has been detected in Type A undifferentiated spermatogonia of Japanese flounder Paralichthys olivaceus [42], medaka Oryzias latipes [43][44][45], roho labeo [35], Nile tilapia [22], and dogfish Scyliorhinus canicula [46], as well as in primordial germ cells (PGCs) and pluripotent embryo stem cells of Japanese flounder [42] and medaka [43][44][45]. Vasa is a highly conserved member of the DEAD box protein family of putative RNA helicases [47], and it is expressed in PGCs of zebrafish Danio rerio [48][49][50][51][52], rainbow trout Oncorhynchus mykiss [53,54], Nile tilapia [55], and sturgeons [56], as well as in undifferentiated Type A spermatogonia of zebrafish [57,58], rainbow trout [59,60], Nile tilapia [22,61], medaka [62], roho labeo [35], Japanese amberjack Seriola quinqueradiata [63], tambaqui Colossoma macropomum [64], and salmonids [65]. The glycosyl phosphatidylinositol-linked ligand-binding subunit GFRA1, a component of the receptor complex binding the glial-cell-line derived neurotrophic factor (GDNF) [30,66,67] has been found in Type A undifferentiated spermatogonia of Nile tilapia [22], rainbow trout [6...…”

Section: Discussionmentioning

confidence: 99%

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation

Zupa

Martino

Marzano

et al. 2020

Animals

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The meagre, Argyrosomus regius, is a valued fish species of which aquaculture production might be supported by the development of a stem germ cell xenotransplantation technology. Meagre males were sampled at a fish farm in the Ionian Sea (Italy) at the beginning and end of the reproductive season. Small and large Type A undifferentiated spermatogonia were histologically identified in the germinal epithelium. Among the tested stemness markers, anti-oct4 and anti-vasa antibodies labeled cells likely corresponding to the small single Type A spermatogonia; no labeling was obtained with anti-GFRA1 and anti-Nanos2 antibodies. Two types of single A spermatogonia were purified via density gradient centrifugation of enzymatically digested testes. Testes from fish in active spermatogenesis resulted in a more efficient spermatogonial stem cell (SSC) yield. After cell seeding, meagre SSCs showed active proliferation from Day 7 to Day 21 and were cultured up to Day 41. After cryopreservation in dimethyl-sulfoxide-based medium, cell viability was 28.5%. In conclusion, these results indicated that meagre SSCs could be isolated, characterized, cultured in vitro, successfully cryopreserved, and used after thawing. This is a first step towards the development of a xenotransplantation technology that might facilitate the reproduction of this valuable species in captivity.

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Isolation and characterization of a germ cell marker in teleost fish Colossoma macropomum

Cited by 11 publications

References 53 publications

Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination

Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination

Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper (Plectropomus leopardus)

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation

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