Isolation and characterization of a California encephalitis serogroup orthobunyavirus from Finnish mosquitoes

Putkuri, Niina; Kurkela, Satu; Levanov, Lev; Huhtamo, Eili; Vaheri, Antti; Sironen, Tarja; Vapalahti, Olli

doi:10.1016/j.meegid.2014.01.023

Cited by 23 publications

(39 citation statements)

References 25 publications

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“…(Text table) Four emerging arboviruses in North America in the Orthobunyavirus genus of the Bunyaviridae family The California serogroup viruses circulate widely throughout the world. They include the Inkoo virus in Europe; the Tahyna viruses in Europe, Asia and Africa; and the La Crosse, snowshoe hare and Jamestown Canyon viruses in North America (4,5). Several California serogroup viruses are associated with mild flu-like diseases and severe central nervous system infections (4,6,7,8).…”

Section: Introductionmentioning

confidence: 99%

Emerging mosquito-borne bunyaviruses in Canada

Drebot¹

2015

CCDR

103

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Section: Introductionmentioning

confidence: 99%

Emerging mosquito-borne bunyaviruses in Canada

Drebot¹

2015

CCDR

103

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“…This is consistent with the view that TAHV is widespread in Europe, 30 Russia, 31 Africa, [32][33][34][35] and Asia (southern Siberia and the Far East, Turkey, Armenia, Azerbaijan, Tadjikistan, Uzbekistan, and China), 30,[36][37][38] whereas INKV and CHATV may be restricted to northern Europe and Asia. 2,30,39 In the endemic region of Europe, approximately 5% of the general population are seropositive for TAHV, 40 and very high frequency (60-80%) of neutralizing antibodies have been reported in elderly persons in the endemic foci. 30 In this study, we detected eight TAHV seropositive individuals (2% of the study population) in southern Iraq, suggesting that the virus also circulates in the Middle East region, even though the human exposure to this virus may be lower than in the central Europe.…”

Section: Discussionmentioning

confidence: 99%

“…The virus strains WNV Eg101, USUV, SINV EgAr339, CHIKV, INKV KN3641, 1 TAHV Bardos strain 92, BATV Calovo strain (no. 184), CHATV Möhkö strain (M07-1) 2 were used in IFA and NTs. SATV was kindly provided by Richard Elliott (MRC-University of Glasgow).…”

Section: Methodsmentioning

confidence: 99%

The Presence and Seroprevalence of Arthropod-Borne Viruses in Nasiriyah Governorate, Southern Iraq: A Cross-Sectional Study

Barakat¹,

Smura²,

Kuivanen³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. The knowledge on the presence and seroprevalence of arboviruses in Iraq is fragmental. To assess the exposure of the population to arbovirus infections in southern Iraq, we conducted a serological screening of the most common arbovirus groups using immunofluorescence, hemagglutination inhibition and neutralization tests. Serum samples of 399 adult volunteers were collected in Nasiriyah, Iraq. Antibodies were detected against West Nile virus (WNV) (11.6%), sandfly-borne Sicilian virus serocomplex (18.2%), sandfly-borne Naples virus serocomplex (7.8%), Sindbis virus (1.5%), chikungunya virus (0.5%), and Tahyna virus (2.0%). The results suggest that WNV and sandfly-borne phlebovirus infections are common in southern Iraq, and these viruses should be considered as potential causative agents in patients with febrile disease and/or neurological manifestations.

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“…It is assumed that most infected persons are asymptomatic or present a mild febrile illness, which is self-limiting in most cases. However, in severe cases, it may cause a neuroinvasive disease that manifest in a form of encephalitis [5, 13]. The sero-prevalence of INKV is high among human populations in endemic areas of Finland (51%) and Sweden (41%) [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Mosquito-borne Inkoo virus in northern Sweden - isolation and whole genome sequencing

Lwande

Bucht

Ahlm

et al. 2017

Virol J

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BackgroundInkoo virus (INKV) is a less known mosquito-borne virus belonging to Bunyaviridae, genus Orthobunyavirus, California serogroup. Studies indicate that INKV infection is mainly asymptomatic, but can cause mild encephalitis in humans. In northern Europe, the sero-prevalence against INKV is high, 41% in Sweden and 51% in Finland. Previously, INKV RNA has been detected in adult Aedes (Ae.) communis, Ae. hexodontus and Ae. punctor mosquitoes and Ae. communis larvae, but there are still gaps of knowledge regarding mosquito vectors and genetic diversity. Therefore, we aimed to determine the occurrence of INKV in its mosquito vector and characterize the isolates.MethodsAbout 125,000 mosquitoes were collected during a mosquito-borne virus surveillance in northern Sweden during the summer period of 2015. Of these, 10,000 mosquitoes were processed for virus isolation and detection using cell culture and RT-PCR. Virus isolates were further characterized by whole genome sequencing. Genetic typing of mosquito species was conducted by cytochrome oxidase subunit I (COI) gene amplification and sequencing (genetic barcoding).ResultsSeveral Ae. communis mosquitoes were found positive for INKV RNA and two isolates were obtained. The first complete sequences of the small (S), medium (M), and large (L) segments of INKV in Sweden were obtained. Phylogenetic analysis showed that the INKV genome was most closely related to other INKV isolates from Sweden and Finland. Of the three INKV genome segments, the INKV M segment had the highest frequency of non-synonymous mutations. The overall G/C-content of INKV genes was low for the N/NSs genes (43.8–45.5%), polyprotein (Gn/Gc/NSm) gene (35.6%) and the RNA polymerase gene (33.8%) This may be due to the fact that INKV in most instances utilized A or T in the third codon position.ConclusionsINKV is frequently circulating in northern Sweden and Ae. communis is the key vector. The high mutation rate of the INKV M segment may have consequences on virulenceElectronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0725-5) contains supplementary material, which is available to authorized users.

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Isolation and characterization of a California encephalitis serogroup orthobunyavirus from Finnish mosquitoes

Cited by 23 publications

References 25 publications

Emerging mosquito-borne bunyaviruses in Canada

Emerging mosquito-borne bunyaviruses in Canada

The Presence and Seroprevalence of Arthropod-Borne Viruses in Nasiriyah Governorate, Southern Iraq: A Cross-Sectional Study

Mosquito-borne Inkoo virus in northern Sweden - isolation and whole genome sequencing

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