Isolation and Characterization of a 1,3-Dichloro-2-Propanol-Degrading Bacterium

Abstract

Arthrobacter sp. strain PY1, a bacterium having the ability to degrade 1,3-dichlotopropanol (1,3-DCP), was isolated from a soil sample of a chemical plant. Strain PY1 degraded 1000 mg/l (7.75 mM) of 1,3-DCP completely within 7 days, and the ability was elevated by acclimatization up to 4000 mg/l/week. Addition of nutrients such as peptone, glucose or glycerol showed no or slight effect on the degrading activity. These results suggest that strain PY1 is a useful organism in a biological control system for 1,3-DCP pollution. The ability to degrade 1,3-DCP was induced by addition of 1,3-DCP to the culture of strain PY1. A 1,3-DCP-degrading enzyme (Deh-PY1) was purified from the cytoplasmic fraction of strain PY1 by fractionation with ammonium sulfate, hydrophobic chromatography and anion exchange chromatography. Purified Deh-PY1 is a tetramer of a homogeneous subunit having a molecular weight of 20 kDa. Analysis of the N-terminal amino acid sequence of Deh-PY1 showed that the 31 residues were quite similar to those of known 1,3-DCP-dehalogenases of other organisms, Arthrobacter sp. strain AD2 and Corynebacterium sp. strain N-1074, although some differences in composition or enzymatic characteristics were observed. The Km value and Vmax of Deh-PY1 were 2.67 mM and 7.81 µmol/min/mg, respectively, and the optimum reaction temperature and pH were 40-50°C and 9.5-10.5.

Biodegradation of oil-based paint by Bacillus species monocultures isolated from the paint warehouses

“…Mineral salt medium (MSM) was modified from Yonetani et al (2004) O, 15; and agar, 12 (in case of MSM agar only). All the salt solutions were separately prepared and autoclaved at 121°C, 15 lb pressure for 15 min, and dissolved one by one into the main MSM medium (approximately 980 ml of distilled water).…”

Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

“…In order to enrich oil-based paint-degrading bacteria, a predetermined amount (3%) of oil-based paint stock solution was added to a 250 ml conical flask to obtain the final concentration of (300 ppm/ml) and the flask was filled with 100 ml sterilized mineral salt medium (MSM) of determined composition prepared with slight modification from Yonetani, et al [20], and adjusted to pH 7.0±2 using 6 N HCl and 1 M NaOH solutions. ) was added into the medium for MSM agar only.…”

Enzymatic approaches to the preparation of chiral epichlorohydrin

“…[17][18][19][20][21] Halohydrin dehalogenase, halohydrin epoxidase or hydrogen-halide lyase are alternative names for the haloalcohol dehalogenase. 22 Haloalcohol dehalogenase can be found in several organisms, such as Flavobacterium sp., 23 Agrobacterium radiobacter, 24 Arthrobacter sp., 25,26 Pseudomonas sp., 27 Corynebacterium sp., 28 Alcaligenes sp., 29 Agrobacterium tumefaciens, 30 Agromyces mediolanus, 31 and so on. Most haloalcohol dehalogenases genes were cloned and sequenced.…”