Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

Abstract

The present study sought to explore the bioremediation of oil-based paint from aqueous media using indigenous bacterial isolate Brevibacillus parabrevis strain NAP3 and its toxicity assessment against prokaryotic and eukaryotic biological models. The bacterial isolate was initially screened for bioremediation potential in mineral salts medium containing oil-based paint (conc. 300 ppm, w/v) under shake flask settings. Moreover, the percentage removal of oil-based paint from aqueous media was investigated using a spectrophotometer at 285 nm under two different experimental conditions, i.e., with and without glucose. Evidently, the bacterial isolate displayed maximum oil-based paint removal of 83% in flasks containing glucose as an additional carbon source after 14 days of the treatment, whereas without glucose supplementation it reached up to 78%. The Fourier transform infrared (FTIR) spectra showed noticeable biodegradative changes of the oil-based paint relevant to peaks in the wave number frequency range of 800-600 cm , and 3,200 to 3,400 cm -1 (corresponding to C-H, C-O-N, C-O-C, and CH 2 aliphatic stretch and terminal CH 3 bonds, and N-H bonds, respectively, in treated samples as compared to controls; non-inoculated samples). After treatment, the cellfree supernatants containing oil-based paints displayed reduced cytotoxicity against brine shrimp larvae, phytotoxicity against wild reddish seeds, and antimicrobial activity against selected pathogenic bacteria and fungi, which indicated the possible use of B. parabrevis as potential oil-based paint-degrading bacterium in order to restore paint-polluted environments.

Estimating Total Phenolics in Taraxacum officinale (L.) Extracts

“…According to Noor et al [5] the knowledge of medicinal plants and their methods of use are confined to aged persons above 50-60 years old. Some of the plants have been tested for their antimicrobial activity and were found to be positive [6][7][8][9].…”

Investigating the Antibacterial Activity of POMA Nanocomposites

“…Although the method of NP penetration into bacteria is not known absolutely, studies have proposed that the treatment of bacterial culture with NPs causes changes in the membrane morphology of membrane, causing a considerable rise in its permeability, which in turn disturbs suitable transport through the plasma membrane. The bacterial cells have become unable to accurately regulate the transport through the plasma membrane, leading to cell death [31][32][33][34][35][36][37][38][39][40]. Fig.…”

Isolation and Identification of Resistant Microorganisms from Automotive Paint Sludge

“…The present study showed that each salt medium provided growth conditions for some of the microorganisms found in paint sludge. Micrococcus yunnanensis was isolated from paint sludge in MSM, while in the same medium, Phulpoto et al (27) isolated Brevibacillus parabrevis strains from oil-based paint sludge and Ashwini et al (28) isolated Pseudomonas, Staphylococcus, and Lactobacillus stains. In the direct and serial culture of paint sludge on the NA medium, bacteria, including Rothia amarae, Gor-donia terrae, and Brevibacillus agri were isolated.…”