Isolation and characterization of a pigeonpea cyclophilin (CcCYP) gene, and its over‐expression in Arabidopsis confers multiple abiotic stress tolerance

Plant Water Relations: Absorption, Transport and Control Mechanisms

“…Several HSPs -classified according to their molecular weight -are induced in conditions of water and saline stress, such as HSP70 (the DnaK family), the chaperones GroEL and HSP60, HSP90 and HSP100 and the small HSP (sHSP) (Alamillo et al, 1995;Campalans et al, 2001, Wang et al, 2004. Within these proteins, there is the cyclophilin, which is a chaperone protein with systemic properties and which is highly induced during water stress, conferring multiple tolerances to abiotic stress (Gottschalk et al, 2008;Sekhar et al, 2010). During conditions of stress, the recycling of macromolecules which lose their function to maintain cellular homeostasis is essential.…”

Isolation and in silico characterization of cDNA encoding cyclophilin from etiolated Vigna mungo seedlings

“…The cDNA sequence encoding V. mungo CyP was submitted to the Genbank with the accession number (FN668732). The length of ORF (519 bp) for V. mungo CyP cDNA sequence was identical to the ORFs of pigeon pea (Sekhar et al, 2010), sugarcane (Que et al, 2011) and rice (Kumari et al, 2009). The nucleotide composition revealed that the V. mungo CyP gene was GC rich as it contained 21.39% A, 30.44% C, 29.87% G and 18.30% T residues.…”

“…Transcriptional analysis showed they are mostly 18-23 kDa proteins expressed throughout the plant, and different isoforms are present in all parts of the cell including cytosol, nucleus, mitochondria, secretory pathway, and chloroplast (Romano et al, 2004). The abundance and diversity of CyP isoforms suggests that plant CyPs are involved in a wide variety of cellular processes, including protein folding, mRNA processing, protein trafficking and maturation, signal transduction, and abiotic and biotic stress responses (Marivet et al, 1992;Chou and Gasser, 1997;Godoy et al, 2000;Sekhar et al, 2010;Que et al, 2011).…”

“…Subsequently, CyP genes have been isolated from Vicia faba (Kinoshita and Shimazaki, 1999), Glycine max (Kan et al, 2002), Arabidopsis thaliana (Romano et al, 2004), maize (Marivet SHORT COMMUNICATION et al, 1995), rice (Kumari et al, 2009), Cajanus cajan (Sekhar et al, 2010) and sugarcane (Que et al, 2011). While the A. thaliana genome contains the largest number (29) of CyP genes (Romano et al, 2004), the number of plant CyPs available in databases is small compared to that of other organisms.…”

The Nature of Rainfall at a Typical Semi-Arid Tropical Ecotope in Southern Africa and Options for Sustainable Crop Production

“…Chickpea is also tolerant to drought, producing higher yields in winter than in summer (Saxena et al, 1993;Katerji, et al, 2001;Kumar et al, 2001;Sabaghpour et al, 2006). On the other hand, tolerance to drought in both cowpea (Elhers and Hall 1997;Singh et al, 1999;Singh and Matsui, 2002) and pigeonpea (Gwata and Siambi, 2009;Kumar et al 2011;Sekhar et al 2010) have been reported. Apart from its ability to develop deep roots, cowpea can have reduced leaf size with thick cuticles that reduce water loss (Graham and Vance 2003).…”