Isolation and characterization of a lytic bacteriophage φKp-lyy15 of Klebsiella pneumoniae

Lu, Yujuan; Shi, Huiping; Zhang, Zhe; Han, Fang; Liu, Jinghua; Sun, Yanbo

doi:10.1007/s12250-014-3523-x

Cited by 10 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No viable LPSE1 phage was detected upon heating at 80°C for 60 min. The pH and thermal tolerance of LPSE1 was better than previously reported phages having 4–10 pH and 60°C ( Bao et al, 2011 ), 4–11 pH and 70°C ( Bao et al, 2015 ) and 4–12 pH and 60°C reported by ( Lu et al, 2015 ). Phage application with better range can give a broader window of application in food stuff with lower or higher pH and with or without thermal treatment.…”

Section: Resultscontrasting

confidence: 57%

“…These characteristics show that LPSE1 belongs to Siphoviridae family of bacteriophages. Other members of family Siphoviridae were reported to impart a 50 nm-78.8 nm wide non-capsulated head group and a non-contractile tail of 100 nm-167.7 nm long and 4 nm-9 nm wide ( Carey-Smith et al, 2006 ; O’Flynn et al, 2006 ; Pao et al, 2006 ; Kocharunchitt et al, 2009 ; Bao et al, 2011 ; Lu et al, 2015 ). The members of the Siphoviridae tend to be present in water and are somatic phages with can infect host bacteria at any time and can also attach to host even it is dead ( Grabow, 2001 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, Characterization, and Application of Bacteriophage LPSE1 Against Salmonella enterica in Ready to Eat (RTE) Foods

et al. 2018

View full text Add to dashboard Cite

Salmonella infection is an important foodborne consumer health concern that can be mitigated during food processing. Bacteriophage therapy imparts many advantages over conventional chemical preservatives including pathogen specificity, natural derivation, potency, and providing a high degree of safety. The objective of this study aimed to isolate and characterize a phage that effectively control Salmonella food contamination. Out of 35 isolated phages, LPSE1 demonstrated a broad Salmonella host range, robust lytic ability, extensive pH tolerance, and prolonged thermal stability. The capacity for phage LPSE1 to control Salmonella Enteritidis-ATCC13076 in milk, sausage, and lettuce was established. Incubation of LPSE1 at 28°C in milk reduced recoverable Salmonella by approximately 1.44 log10 CFU/mL and 2.37 log10 CFU/mL at MOI of 1 and 100, respectively, as relative to the phage-excluded control. Upon administration of LPSE1 at an MOI of 1 in sausage, Salmonella count decreased 0.52 log10 at 28°C. At MOI of 100, the count decreased 0.49 log10 at 4°C. Incubation of LPSE1 on lettuce reduced recoverable Salmonella by 2.02 log10, 1.71 log10, and 1.45 log10 CFU/mL at an MOI of 1, 10, and 100, respectively, as relative to the negative control. Taken together, these findings establish LPSE1 as an effective weapon against human pathogenic Salmonella in various ready to eat foods.

show abstract

Section: Resultscontrasting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Isolation, Characterization, and Application of Bacteriophage LPSE1 Against Salmonella enterica in Ready to Eat (RTE) Foods

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Interestingly, in the temperature range from −20 and 70 • C, it was observed that ZCKP8 was relatively thermally stable. ZCKP8 maximal activity was detected at 50 • C, with a moderate decrease of 2 log 10 PFU/mL at 70 • C. K. pneumoniae phages, including wKp-lyy15, phage Z, PKP126, vB_Klp_1, vB_Klp_3, vB_Klp_4, vB_Klp_5, and vB_Klp_6, were previously mentioned to be active at 70 • C [29][30][31].…”

Section: Discussionmentioning

confidence: 84%

“…ZCKP8 maximal activity was detected at 50 °C, with a moderate decrease of 2 log 10 PFU/mL at 70 °C. K. pneumoniae phages, including wKp-lyy15, phage Z, PKP126, vB_Klp_1, vB_Klp_3, vB_Klp_4, vB_Klp_5, and vB_Klp_6, were previously mentioned to be active at 70 °C [ 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Topically Applied Bacteriophage to Control Multi-Drug Resistant Klebsiella pneumoniae Infected Wound in a Rat Model

et al. 2021

View full text Add to dashboard Cite

(Background): Multi-drug-resistant Klebsiella pneumoniae (MDR-KP) has steadily grown beyond antibiotic control. Wound infection kills many patients each year, due to the entry of multi-drug resistant (MDR) bacterial pathogens into the skin gaps. However, a bacteriophage (phage) is considered to be a potential antibiotic alternative for treating bacterial infections. This research aims at isolating and characterizing a specific phage and evaluate its topical activity against MDR-KP isolated from infected wounds. (Methods): A lytic phage ZCKP8 was isolated by using a clinical isolate KP/15 as a host strain then characterized. Additionally, phage was assessed for its in vitro host range, temperature, ultraviolet (UV), and pH sensitivity. The therapeutic efficiency of phage suspension and a phage-impeded gel vehicle were assessed in vivo against a K. pneumoniae infected wound on a rat model. (Result): The phage produced a clear plaque and was classified as Siphoviridae. The phage inhibited KP/15 growth in vitro in a dose-dependent pattern and it was found to resist high temperature (˂70 °C) and was primarily active at pH 5; moreover, it showed UV stability for 45 min. Phage-treated K. pneumoniae inoculated wounds showed the highest healing efficiency by lowering the infection. The quality of the regenerated skin was evidenced via histological examination compared to the untreated control group. (Conclusions): This research represents the evidence of effective phage therapy against MDR-KP.

show abstract

“…In this study, a lytic bacteriophage Ec-ZZ2 infecting Enterococcus faecium isolates was isolated from a sewage sample obtained from Jilin University First-Affiliated Hospital. The bacteriophage was purified by the agar double-layer method (8, 9) and stored at 4°C. It was classified as a member of the family Siphoviridae based on morphological analysis using electron microscopy.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium

Shi

Zhao

et al. 2016

Genome Announc

Self Cite

View full text Add to dashboard Cite

show abstract

Isolation and characterization of a lytic bacteriophage φKp-lyy15 of Klebsiella pneumoniae

Cited by 10 publications

References 15 publications

Isolation, Characterization, and Application of Bacteriophage LPSE1 Against Salmonella enterica in Ready to Eat (RTE) Foods

Isolation, Characterization, and Application of Bacteriophage LPSE1 Against Salmonella enterica in Ready to Eat (RTE) Foods

Topically Applied Bacteriophage to Control Multi-Drug Resistant Klebsiella pneumoniae Infected Wound in a Rat Model

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium

Contact Info

Product

Resources

About