Isolation and Characterization of a Major Allergen from Oriental Mustard Seeds, &lt;i&gt;Bra j&lt;/i&gt;I

Peña, Manuel A. González De La; Menéndez‐Arias, Luis; Monsalve, Rafaél I.; Rodríguez, Roberto Sánchez

doi:10.1159/000235505

Cited by 69 publications

(40 citation statements)

References 16 publications

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“…Most of them are known to cause different types of allergic reactions, based on the route of contact [15][16][17]25]. The seeds and oil o f B. napus and Sina- pis alba have also been reported as important allergens from other countries.…”

Section: Discussionmentioning

confidence: 99%

Immunobiochemical Characterization of Brassica campestris Pollen Allergen

Singh

Verma²,

Rai³

et al. 1995

Int Arch Allergy Immunol

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Brαssica campestris (BC), Eng. Mustard, is an important source of pollen allergen, responsible for type I hypersensitivity disorders. In the present study, BC pollen extract was characterized by TLIEF, SDS-PAGE and immunoprinting. The extract separated into 50 silver stained bands of pi 3-9 on isoelectric focusing whereas it resolved into 14 Coomassie blue stained protein bands of 14–100 kD on SDS-PAGE. Immunoblot analysis with individual patient sera detected four allergenic proteins of 90, 67, 60 and 14 kD. BC separated into 8 peaks (Bras 1–8) on DEAE Sephadex A-50 column. Bras 2 was found to be most potent by IgE specific ELISA, hence further fractionated on Sephadex G-200. A protein of 90 kD (Bras 2a) isolated by gel filtration was found to be most allergenic protein by ELISA inhibition. The findings shall be applicable in standardization of future batches of BC pollen extract to be used for allergy diagnosis and immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Immunobiochemical Characterization of Brassica campestris Pollen Allergen

Singh

Verma²,

Rai³

et al. 1995

Int Arch Allergy Immunol

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“…10,11 More recently, an 11S globulin storage protein of 51 kd has been isolated and identified as a novel major allergen of mustard seeds. 12 The low levels, or even absence, of both groups (2S and 11S) of seed storage proteins in pollens and other plant organs, such as leaves and fruits, lead to the suspicion that these allergen families are probably not responsible for the cross-reactivity among cabbage, mustard, and other plant foods and pollens.…”

mentioning

confidence: 99%

Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens

Palacín¹,

Cumplido²,

Figueroa³

et al. 2006

Journal of Allergy and Clinical Immunology

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“…Bra j 1 is a 2S seed storage albumin similar to Sin a 1 in structure and amino acid composition (Gonzalez de la Peña et al, 1991).…”

Section: Identified Allergensmentioning

confidence: 99%

“…Also six monoclonal antibodies and a rabbit polyclonal serum specific for Sin a 1 recognised the 2S fraction of Brassica juncea, suggesting that Bra j 1 and Sin a 1 may share a homologous epitope and that subjects allergic to one type of mustard may also react to other types (Gonzalez de la Peña et al, 1991;Monsalve et al, 1993;Palomares et al, 2007).…”

Section: Cross-reactivitiesmentioning

confidence: 99%

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2014

EFSA Journal

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Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

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Isolation and Characterization of a Major Allergen from Oriental Mustard Seeds, <i>Bra j</i>I

Cited by 69 publications

References 16 publications

Immunobiochemical Characterization of <i>Brassica campestri</i><i>s</i> Pollen Allergen

Immunobiochemical Characterization of <i>Brassica campestri</i><i>s</i> Pollen Allergen

Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens

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