Isolation and characterization of a lectin with antifungal activity from Egyptian Pisum sativum seeds

Pea lectin inhibits growth of Ehrlich ascites carcinoma cells by inducing apoptosis and G2/M cell cycle arrest in vivo in mice

“…Using this method 20 mg of Pea lectin was purified from 3200 mg of crude proteins whereas 16 mg lectin was purified from 4200 mg of crude protein as described by Sitohy et al (2007). The molecular weight of ␣-subunit and ␤-subunit of Pea lectin was almost the same as described by Sitohy et al (2007). The minimum concentration of Pea lectin required for hemmaglutination activity of rat red blood cells was 8 g/ml.…”

“…Pea lectin highly agglutinated human, rabbit and rat erythrocytes but weekly agglutinated chicken erythrocytes. It was also reported that Pea lectin inhibited the growth of Aspergillus flavus, Trichoderma viride and Fusarium oxysporum (Sitohy et al 2007). The lectin also inhibited the migration of cardiac mesenchymal cells (Sumida et al 1997).…”

“…They are found in all kinds of organisms, including animals, plants, fungi, bacteria and viruses. Plant lectins represent a unique group of proteins with potent biological activities, such as agglutination, toxicity, anti-proliferation of cancer cells as well as having anti-fungal and anti-bacterial activities (Kabir et al 2011a(Kabir et al ,b, 2012Liu et al 2010;Sitohy et al 2007). They can specifically recognize and bind to various sugar structures, and thus trigger several important cellular processes (Sharon and Lis 1989;Sharon 2007).…”

Hemagglutinating activity and conformation of a lactose-binding lectin from mushroom Agrocybe cylindracea

“…Mn 2+ and Mg 2+ also have the capability to restore the activity of ACL, but with a lower extent (75.2%) ( Table 1). These results suggested that ACL require metal ions for its hemagglutinating activity, or the metal ions are tightly bound to the lectin molecule [30]. Conformation changes induced by metal ions were also examined by monitoring its fluorescence emission spectrum of ACL.…”

Isolation and Antifungal Activity of Plant Lectins against some Plant Pathogenic Fungi

“…The lectin isolated from Ophiopogonjaponicus rhizomes was an antifungal agent against the phytopathogens Gibberella saubinetii and R. solani but not on Penicillium italicum (Tian et al, 2007). The lectin of Pisum sativum seeds inhibited the growth of F. oxysporum and Trichoderma viride (Sitohy et al, 2007).…”