Isolation and characterization of a bacteriophage F20 virulent to Enterobacter aerogenes

Mishra, Charitra Kumar; Choi, Tae Hee; Kang, Sun Chul

doi:10.1099/vir.0.043562-0

Cited by 27 publications

(24 citation statements)

References 22 publications

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“…The stability of the isolated TSE bacteriophages was tested at three different temperatures; the bacteriophages remained stable at 4°C and -20°C for a month, while none survived at 37°C. The best storage temperature for the TSE phages was -20°C, as reported by previous authors [14].…”

Section: Discussionsupporting

confidence: 56%

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Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

2016

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The emergence of antibiotic resistant bacterial pathogens is becoming a major challenge for patient care. The utilization of alternative therapies for infectious diseases other than antibiotics is an urgent need of today medical practice. The utilization of lytic bacteriophages and their gene products as therapeutic agents against antibiotic resistant bacteria is one of the convincing alternative approaches. Here we present the isolation and characterization of three lytic bacteriophages TSE1-3 against

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Section: Discussionsupporting

confidence: 56%

“…This moderate burst size favors their use in the phage therapy and coincide with other phages against Enterobacter species [19]. The physiological stage of the host cell will also have effects on the burst size of phages and this factor should explored in future research [20].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

2016

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“…These results suggest that free bacteriophages might not be stable in gastric condition. In general, bacteriophages are stable between pH 5.0 and 8.0 (Chow & Rouf, 1983;Mishra et al, 2012;Pouillot et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Application of chitosan–alginate microspheres for the sustained release of bacteriophage in simulated gastrointestinal conditions

Kim

Ahn

2015

Int J of Food Sci Tech

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Summary This study was designed to evaluate the acid stability, release property and antimicrobial efficacy of Escherichia coli O157:H7 bacteriophages encapsulated in chitosan–alginate microspheres under the simulated gastrointestinal conditions. The bacteriophages belonging to Myoviridae family were stable at the pH above 4 in trypticase soy broth. The chitosan–alginate microspheres exhibited protective effect on the viability of bacteriophages in the simulated gastric conditions at pH 2.0 and pH 2.5, showing 4.8 and 5.6 log PFU mL‐1, respectively, after 1 h of incubation at 37 °C. The release per cent of bacteriophages from microspheres gradually increased up to 65% in the simulated intestinal condition (pH 7.5) at 37 °C for 6 h. The lytic efficacy of chitosan‐ and alginate‐encapsulated bacteriophages against E. coli O157:H7 was significantly maintained in the simulated intestinal conditions to 10 h of incubation (1.3 log reduction). The results suggest that the chitosan–alginate microspheres can be used as a reliable delivery system for bacteriophages.

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“…The bacteriophage was allowed to adsorb for 15 min at 37°C [9]. The mixture was then centrifuged at 12,000 × g for 30 s to remove unadsorbed phage particles, and the resultant pellet was re-suspended in 5 ml of LB medium.…”

Section: Resultsmentioning

confidence: 99%

Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates

Peng

Huang

et al. 2014

BMC Microbiol

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BackgroundWith the use of broad-spectrum antibiotics, immunosuppressive drugs, and glucocorticoids, multidrug-resistant Acinetobacter baumannii (MDR-AB) has become a major nosocomial pathogen species. The recent renaissance of bacteriophage therapy may provide new treatment strategies for combatting drug-resistant bacterial infections. In this study, we isolated a lytic bacteriophage vB_AbaM-IME-AB2 has a short latent period and a small burst size, which clear its host’s suspension quickly, was selected for characterization and a complete genomic comparative study.ResultsThe isolated bacteriophage vB_AbaM-IME-AB2 has an icosahedral head and displays morphology resembling Myoviridae family. Gel separation assays showed that the phage particle contains at least nine protein bands with molecular weights ranging 15–100 kDa. vB_AbaM-IME-AB2 could adsorb its host cells in 9 min with an adsorption rate more than 99% and showed a short latent period (20 min) and a small burst size (62 pfu/cell). It could form clear plaques in the double-layer assay and clear its host’s suspension in just 4 hours. Whole genome of vB_AbaM-IME-AB2 was sequenced and annotated and the results showed that its genome is a double-stranded DNA molecule consisting of 43,665 nucleotides. The genome has a G + C content of 37.5% and 82 putative coding sequences (CDSs). We compared the characteristics and complete genome sequence of all known Acinetobacter baumannii bacteriophages. There are only three that have been sequenced Acinetobacter baumannii phages AB1, AP22, and phiAC-1, which have a relatively high similarity and own a coverage of 65%, 50%, 8% respectively when compared with our phage vB_AbaM-IME-AB2. A nucleotide alignment of the four Acinetobacter baumannii phages showed that some CDSs are similar, with no significant rearrangements observed. Yet some sections of these strains of phage are nonhomologous.ConclusionvB_AbaM-IME-AB2 was a novel and unique A. baumannii bacteriophage. These findings suggest a common ancestry and microbial diversity and evolution. A clear understanding of its characteristics and genes is conducive to the treatment of multidrug-resistant A. baumannii in the future.

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Isolation and characterization of a bacteriophage F20 virulent to Enterobacter aerogenes

Cited by 27 publications

References 22 publications

Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

Application of chitosan–alginate microspheres for the sustained release of bacteriophage in simulated gastrointestinal conditions

Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates

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