Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities

Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

“…With the advancement in different molecular biotechnologies, a heap of molecular‐based prophylactic and/or therapeutic agents has been reported. These include the technology of RNA interference (Itsathitphaisarn, Thitamadee, Weerachatyanukul, & Sritunyalucksana, ; Jitrakorn, Chucherd, & Saksmerprome, ; Reshi, Wu, Wang, & Hong, ), recombinant DNA technology (Kumar, Karunasagar, & Karunasagar, ; Sahoo et al., ) and technology of aptamers that is also known as the synthetic antibody (Aoki & Hirono, ; Hwang et al., ; Li et al., ; Li, Zhou, et al., ; Zhou et al., ). Scanty reports of other approaches, for instances the assessment of genetic variation/inheritance in selective breeding of disease‐resistant strains (Luan et al., ) and the use of natural products of plant extracts (Farook et al., ; Khanna, Kannabiran, Babu, & Hameed, ), are available.…”

“…To date, the application of aptamers technology in infectious disease diagnosis of aquatic animal is mostly limited to viral infections. Several aptamers have been reported for viral detections (Aoki & Hirono, ; Li et al., ; Li, Zhou, et al., , Zhou et al., , ) and the detection of virus‐infected cells (Li, Wei, et al., ; Li et al., , ), and aptamers that manifesting antiviral property (Hwang et al., ; Li et al., ; Li, Zhou, et al., ; Zhou et al., ).…”

Comparative Pathology of Ranaviruses and Diagnostic Techniques

“…Allender et al ( 2012 b) reported the possibility of using guanine analogue antiviral drugs (acyclovir and valacyclovir) to treat chelonians for infection with iridoviruses and herpesviruses. Recently, Li et al ( 2014a ) reported antiviral activity of DNA aptamers when treating SGIV. Heat treatment is effective at inactivating many pathogens of ectothermic vertebrates such as Batrachochytrium dendrobatidis (Woodhams et al 2003 ).…”

Development of novel aptamer‐based enzyme‐linked apta‐sorbent assay (ELASA) for rapid detection of mariculture pathogen Vibrio alginolyticus

“…At present, aptamers have made great progress in the ichthyopathology field. The aptamers against some severe aquatic viruses, such as Singapore grouper iridovirus (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ), red‐spotted grouper nervous necrosis virus (Zhou et al, ), Trachinotus ovatus NNV (Yu, Liu, Wei, et al, ), spring viraemia of carp virus (Yu et al, ), Hirame rhabdovirus virus (Hwang et al, ), viral haemorrhagic septicaemia virus (Punnarak et al, ) and soft‐shelled turtle iridovirus (Li, Wei, et al, ; Li, Zhou, et al, ), have been successfully generated. There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ).…”

“…There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ). Aptamers have been widely applied in different important research areas, including drug‐specific delivery (Loo et al, ; Zhao et al, ), pathogen diagnosis (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ; Teng et al, ; Yu, Liu, Wei, et al, ; Zhou et al, ), toxin analysis (Huang et al, ; Yan et al, ) and disease pathogenesis research (Bunka & Stockley, ). The excellent characteristics of easy synthesis, low immunogenicity, innocuity and high specificity and stability make aptamers ideal alternatives to antibodies (Toh et al, ).…”