Isolation and Characterization of a Strain of<i>Bacillus megaterium</i>That Degrades Poly(vinyl alcohol)

Mori, Tatsuma; Sakimoto, Michio; Kagi, Takashi; Sakai, Taku

doi:10.1271/bbb.60.330

Cited by 82 publications

(45 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phylogenetic and physiological analyses reveal that strains iso- (48)(49)(50)(51)(52)(53). In light of the diversity of Bacillus strains able to grow under scCO 2 , we hypothesize that the ability to grow under high pCO 2 is a widespread feature of Bacillus spp.…”

Section: Discussionmentioning

confidence: 94%

Microbial Growth under Supercritical CO ₂

Peet

Freedman

Hernandez

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Growth of microorganisms in environments containing CO 2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. Thus, supercritical CO 2 (scCO 2 ) is generally regarded as a sterilizing agent. We report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (GCS) that are capable of growth in pressurized bioreactors containing scCO 2 . Analysis of 16S rRNA genes from scCO 2 enrichment cultures revealed microbial assemblages of varied complexity, including representatives of the genus Bacillus. Propagation of enrichment cultures under scCO 2 headspace led to isolation of six strains corresponding to Bacillus cereus, Bacillus subterraneus, Bacillus amyloliquefaciens, Bacillus safensis, and Bacillus megaterium. Isolates are spore-forming, facultative anaerobes and capable of germination and growth under an scCO 2 headspace. In addition to these isolates, several Bacillus type strains grew under scCO 2 , suggesting that this may be a shared feature of spore-forming Bacillus spp. Our results provide direct evidence of microbial activity at the interface between scCO 2 and an aqueous phase. Since microbial activity can influence the key mechanisms for permanent storage of sequestered CO 2 (i.e., structural, residual, solubility, and mineral trapping), our work suggests that during GCS microorganisms may grow and catalyze biological reactions that influence the fate and transport of CO 2 in the deep subsurface.

show abstract

Section: Discussionmentioning

confidence: 94%

Microbial Growth under Supercritical CO ₂

Peet

Freedman

Hernandez

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…There were different species of bacteria and fungi according to the type of buried membrane and the concentration of the polymer. The buried chromated pure PVA membrane, the dominant species were Bacillus spp [25,45,46,47,48,49], Pseudomonas spp [27,31,50,47,48], Aspergillus spp [51] and Penicillium spp [27,47]. In addition, for the buried GA, the major species were Bacillus spp [6,12,45], Penicillium spp, Aspergillus spp, Rhizorpous spp, [6,33,34].…”

Section: Biodegradation Bacteria and Fungimentioning

confidence: 99%

“…This results agree with Coleman and Paran [52] who concluded that Gram-negative chromium reducing-bacteria tend to be more sensitive to chromium ion, while Gram positive bacteria are significantly tolerant to chromate ion toxicity even at relatively high concentration of the ion [25,52]. Moreover, the microbial communities of the bioplastic blends, namely (chromated GA/PVA=1:1), (chromated GA/PVA=1:0.75), (chromated GA/PVA=0.5) and (GA/PVA=1:0.25) contained Bacillus spp [25,45,46,49,53], Pseudomonas spp [27,31,47,48,50], Aspergillus spp, Rhizorpous spp, and Penicillium spp. [33].…”

Section: Biodegradation Bacteria and Fungimentioning

confidence: 99%

Effect of Potassium Dichromate on Properties and Biodegradation of Gum Arabic Based Bioplastic Membranes

Hindi¹,

Albureikan²,

Alghamdi³

et al. 2017

NNR

View full text Add to dashboard Cite

Gum Arabic (GA) collected from Acacia senegal trees was used with polyvinyl alcohol (PVA) to prepare of a series of biodegradable membranes doped and non-doped with potassium dichromate (K 2 Cr 2 O 7 ). Adding the K 2 Cr 2 O 7 to the GA/PVA blends slightly decreased their crystallinity index (CI) by about 2 %. Increasing the PVA concentration in the chromated GA/PVA blends was responsible for increasing the CI. Adding the K 2 Cr 2 O 7 to the pure GA solution modified its differential thermal behavior whereby the exothermic reactions occurred between 321°C and 433°C were disappeared. The K 2 Cr 2 O 7 increased the heat change drastically for all the bioplastic blends with the highest increase for the pure GA. Adding K 2 Cr 2 O 7 to the pure PVA increased the nanometric particle size (NPS) significantly. Increasing the PVA concentration in a blend had a greater effect than did the K 2 Cr 2 O 7 on the NPS. The buried bioplastic membranes in the control soil had different count and species of microbial communities. The numbers of bacteria and fungi in the initial soil sample were lower than those for chromated GA membranes and were greater than those for the chromated PVA. All bacterial and fungi species had growth ability and are expected to be detoxification tools of chromium ion-doped blends of GA and PVA leading to a green environment.

show abstract

“…Polyvinyl alcohol (PVA) with its carbon-carbon backbone, being water soluble can be biodegraded under both aerobic and anaerobic conditions (Suzuki et al 1973;Watanabe et al 1976;Sakazawa et al 1981;Shimao et al 1983;Mori et al 1996;Matsumura et al 1993) and therefore finds application in the manufacture of carriers used in delivery system for chemicals such as fertilizer, pesticides (Premraj and Doble 2005), in the textile, papercoating and chemical industries (Qian et al 2004) and also as the biodegradable backbone in sugar and fatty acidbranched polymers (Tokiwa et al 2000). The annual world production of PVA is about 0.65 million tons/year, and a large amount of the used and discarded PVA also poses a significant waste problem (Tokiwa et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…They include Pseudomonas O-3 (Suzuki et al 1973), Pseudomonas sp. (Shimao et al 1984(Shimao et al , 1986(Shimao et al , 1996Watanabe et al 1975Watanabe et al , 1976Sakai et al 1981Sakai et al , 1985a, Alcaligenes faecalis (Matsumura et al 1993) and Bacillus megaterium (Mori et al 1996). Among fungi, Penicillium sp.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Raghul

Bhat

Chandrasekaran

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Marine bacteria, Vibrio alginolyticus and Vibrio parahemolyticus isolated from sediments were evaluated for their ability as a consortia, to degrade polyvinyl alcohol-low linear density polyethylene (PVA-LLDPE)-blended plastic films in shake flask conditions at 120 rpm at 37°C over 15 weeks. Results indicated that relatively 20 % decrease in tensile strength of the film could be achieved with 25 and 30 % blend of PVA in the PVA-LLDPE plastic film compared to other ratios. Micrographs obtained with scanning electron microscope showed visible cracks and grooves on the surface of the PVA-LLDPE blend film after 15 weeks of incubation with bacterial consortium. The decrease in tensile strength of the PVAblended plastic films after treatment and the results of the scanning electron microscopic analysis evidence that the consortium could cause degradation of PVA-LLDPE plastic blends compared to suitable controls. This is the first report on polyvinyl alcohol degrading Vibrio sp. from marine sediments and its application in microbial degradation of polyvinyl alcohol-low linear density polyethylene plastic blends. The study indicated potential of marine benthic vibrios that have novel enzymes and unique characteristics for application in bioremediation and solid waste management particularly in handling synthetic polymers such as PVA-blended plastic films.

show abstract

Isolation and Characterization of a Strain ofBacillus megateriumThat Degrades Poly(vinyl alcohol)

Cited by 82 publications

References 3 publications

Microbial Growth under Supercritical CO ₂

Microbial Growth under Supercritical CO ₂

Effect of Potassium Dichromate on Properties and Biodegradation of Gum Arabic Based Bioplastic Membranes

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Contact Info

Product

Resources

About

Isolation and Characterization of a Strain ofBacillus megateriumThat Degrades Poly(vinyl alcohol)

Cited by 82 publications

References 3 publications

Microbial Growth under Supercritical CO 2

Microbial Growth under Supercritical CO 2

Effect of Potassium Dichromate on Properties and Biodegradation of Gum Arabic Based Bioplastic Membranes

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Contact Info

Product

Resources

About

Microbial Growth under Supercritical CO ₂

Microbial Growth under Supercritical CO ₂