Isolation and characterization of a rat skin parvalbumin-like calcium-binding protein

Rinaldi, Murielle L.; Haiech, Jacques; Pavlovitch, J. H.; Rizk, M.; Ferraz, Conchita; Derancourt, Jean; Demaille, Jacques

doi:10.1021/bi00262a044

Cited by 48 publications

(16 citation statements)

References 31 publications

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“…A significant increase of xz was observed whenever the ratio of Kd2 and K,, was higher than 2; thus, the binding sites are equivalent within this factor. rat parvalbumin in the presence of fluo-3 could be fitted assuming two equivalent, non-interacting sites, in agreement with other measurements (Rinaldi et al, 1982;Henzl and Birnbaum, 1988;Pauls et al, 1993). Systematic deviations of the theoretical curve from the experimental data are observed when asymmetric sites are assumed (Fig.…”

Section: Ca2+ and Mg2+ Binding To Parvalbuminsupporting

confidence: 87%

“…Parvalbumins contain two Ca2+/Mgz+ binding sites of the EF-hand type (Declercq et al, 1988) which exhibit high affinity for Caz+ and moderate affinity for Mg". Measurements of Ca2+ and Mg2+ binding revealed that some parvalbumins contain functionally equivalent sites binding Ca2+ and Mg2+ in a competitive fashion (Haiech et al, 1979;Rinaldi et al, 1982;Henzl and Birnbaum, 1988) whereas others exhibit two high-affinity Ca2+/Mg2' sites with different properties or one Ca2+/Mg2+ site of high-affinity and a Ca" site of lower affinity which is more specific for Ca2+ (Permyakov et al, 1987;Henzl and Birnbaum, 1988;Eberspach et al, 1988). It is generally believed that the high-affinity Ca2+/ Mg2+ sites constitute an important intracellular Ca2+ buffer whereas the Ca2+ sites may confer regulatory functions similar to those of calmodulin and troponin C (Henzl and Birnbaum, 1988).…”

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confidence: 99%

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Calcium and magnesium binding to rat parvalbumin

Eberhard

Erné

1994

European Journal of Biochemistry

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Ca2+ and Mg2+ binding to rat parvalbumin was measured by means of the fluorescent Ca2+ indicator fluo‐3 using a method developed earlier [Eberhard, M. & Erne, P. (1991) Eur. J. Biochem. 202, 1333–1338]. We demonstrate that rat parvalbumin contains two equivalent Ca2+/Mg2+ binding sites and that Ca2+ and Mg2+ compete for the same sites. Dissociation constants (Kd) for Ca2+ and Mg2+ in Hepes buffer containing 150 mM K+ at 35°C and pH 7.2 are 11.0 ± 1.8 nM and 41 ± 8 μM, respectively. At an ionic strength below 0.2 M, Kd values of Ca2+ binding to rat parvalbumin are approximately proportional to the ion concentration. Kd values of Ca2+ binding were found to be about fourfold larger in the presence of Na+ as compared with K+, indicating that Na+ distinctly influences Ca2+ binding to rat parvalbumin. Both Ca2+ and Mg2+ binding to parvalbumin are exothermic whereas Ca2+ and Mg2+ binding to fluo‐3 are endothermic entropy‐driven processes.

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Section: Ca2+ and Mg2+ Binding To Parvalbuminsupporting

confidence: 87%

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confidence: 99%

Calcium and magnesium binding to rat parvalbumin

Eberhard

Erné

1994

European Journal of Biochemistry

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“…Rabbit 3 was inoculated with SCaBP prepared as described by Rinaldi et al [25]. The method of preparing the antiserum is described by Laouari et al 1201.…”

Section: Materials and Methods Antiseramentioning

confidence: 99%

Molecular cloning of mouse epidermal cystatin A and detection of regulated expression in differentiation and tumorigenesis

Hawley‐Nelson

Roop

Cheng

et al. 1988

Molecular Carcinogenesis

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A gamma gt 11 cDNA expression library representing mouse epidermis mRNA was screened with polyclonal rabbit antiserum directed against 10-13 kDa epidermal antigens that had previously been shown to be regulated during epidermal differentiation. A cDNA clone was detected and isolated and its identity as the coding sequence for one of the antigens was confirmed by translation of hybrid-selected mRNA from mouse epidermis. The cDNA sequence predicted a peptide homologous to the reported sequence of rat epidermis cystatin A, a thiol proteinase inhibitor. This identification was confirmed by cross-reactivity of the gamma clone fusion protein with authentic antiserum to rat epidermis cystatin A. Southern gel analysis showed that mouse DNA may contain several closely related genes homologous to the cystatin probe. An mRNA of about 0.6 kb from epidermis and cultured mouse epidermal cells hybridized with the cystatin probe on northern analysis. The abundance of the message was high in cultured basal cells and was selectively diminished by inducing terminal differentiation in culture with an elevated Ca2+ concentration in the medium. Cystatin message was abundant in chemically induced mouse skin papillomas but reduced in carcinomas. In epidermis, mRNA was localized to the less differentiated basal and lower spinous layers by in situ hybridization. Regulation of expression of cystatin A in epidermis and tumors suggests that it may be important in the control of normal keratinocyte proliferation and differentiation and in malignant conversion.

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“…In recent years intracellular calcium-binding proteins and calcium-dependent proteins have been examined in animal epidermis. Rat skin calcium binding protein has been speculated to involve the control of the basal cell proliferation from the immunohistochemical observation (4,20). The level of calmodulin, which is highly conserved throughout animal and plant species, increased in the in vivo hyperproliferative state (15), psoriasis, and in the in vitro low calcium-induced hyperproliferative state (8).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it requires cautiousness to discuss this sequence of calcium-induced differentiation of keratinocytes in vitro with relation to the intracellular event that calcium and calciumrelated agents regulate. There have been several studies which focussed on calciumbinding proteins, such as calmodulin (8,15) and on the other calcium-related agents (4,14,20). Ca++-ATPase activity, an energy dependent calcium extrusion pump, is well accepted to reflect intracellular calcium level.…”

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confidence: 99%