Calinodulin (CaM) has been implicated as a multifunctional regulator of Ca2 in the cytoplasm of eels. We have recently introduced biologically active colloidal gold-labeled CaM as a marker for identifying potential CaM binding sites (unoccupied by endogenous CaM at the time offixation) by dcctron microscopy and have stained frozen thin sections of rat cardiac muscle with this conjugate. In the presence of Ca2 , gold partides indicating CaM binding sites were found localized on the sarcoplasmic reticulum, mitochondna, and gap 1 Supported in part by a grant from the)apanese Government No.

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