Isolation and characterization of a novel polysaccharide from the mucus of the loach, Misgurnus anguillicaudatus

Qin, Chuanguang; Huang, Kaixun; Xu, Huibi

doi:10.1016/s0144-8617(01)00335-6

Cited by 63 publications

(32 citation statements)

References 17 publications

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“…Ethanol (3200 ml, 95% v/v) was slowly added to the condensed solution and left 12 h at 4°C. The resulting precipitate was collected, then dissolved with 200 ml of water and subjected to Sevag method to remove free protein [10]. The deproteinization solution was re-precipitated in 95% ethanol of four times volume.…”

Section: Sample Processingmentioning

confidence: 99%

Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

Xiaoming

et al. 2009

Plant Foods Hum Nutr

153

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The antioxidative activities of water extract (WE) and crude hot-water soluble polysaccharide (PS) from Ficus carica L. fruit were investigated using various assays in vitro, including scavenging abilities on DPPH, superoxide and hydroxyl radicals and reducing power. The immunity activities of PS were evaluated using the carbon clearance test and serum hemolysin analysis in mice. In addition, total phenolics and flavonoids contents were also determined. Both WE and PS have notable scavenging activities on DPPH with the EC(50) values of 0.72 and 0.61 mg/ml, respectively. The PS showed higher scavenging activity than WE on superoxide radical (EC(50), 0.95 mg/ml) and hydroxyl anion radical (scavenging rate 43.4% at concentration of 4 mg/ml). The PS (500 mg/kg) also has a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice. The results indicate that both WE and PS might be applicable in healthy medicine and food industry.

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Section: Sample Processingmentioning

confidence: 99%

Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

Xiaoming

et al. 2009

Plant Foods Hum Nutr

153

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“…After centrifuging to remove debris fragments, the solution was concentrated in a rotary evaporator. Protein was removed with the Sevag method [12]. Then, the solution was precipitated with three volumes of 95% ethanol for 24 h at 4°C.…”

Section: Isolation For Polysaccharidementioning

confidence: 99%

Characterization and antitumor activity of pollen polysaccharide

Yang

Guo

Zhang³

et al. 2007

International Immunopharmacology

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“…Our results indicated that extracting temperature has significant influence on the yield of the polysaccharides. In most of procedures, polysaccharide was usually extracted at 80℃ (Qin et al, 2002;Xie et al, 2007). The experiments suggested that there was significant difference for yield of the polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes

Zhao

Liu

et al. 2013

FSTR

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Orthogonal experiment was used to optimize the extraction conditions of Flammulina velutipes mycelia polysaccharides (FvP). Four independent variables (ratio of water to raw material, initial pH value, extracting temperature, and extracting time) were taken into consideration. A yield of FvP of 2.19% was obtained under an optimized condition (ratio of water to material of 50:1, initial pH value of 6.0, extracting temperature of 85℃, and extracting time of 6 h). Subsequently, antioxidative properties of FvP-2 (crude polysaccharides) and FvP-3 (deproteinized polysaccharides) prepared from F. velutipes mycelia were evaluated by monitoring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical scavenging activity, together with antitumor activity against the human hepatocellular carcinoma BEL-7402 cells. DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. The inhibition rate of BEL-7402 cells was up to 45% at 640 μg/mL of FvP-2. These results suggested that FvP possesses potent antioxidant and antitumor properties. The polysaccharide may be useful as a functional food additive and an antioxidant and antitumor agent. IntroductionMushrooms have been valued as edible and medicinal resources, and antitumor substances have been identified in many mushroom species. Polysaccharides, as one of the most important components isolated from mushrooms, have been correlated with multiple pharmacological activities, such as antioxidant, immunomodulatory, reducing blood lipid and antitumor activities. Flammulina velutipes, one of the most popular edible mushrooms, has being under a large-scale artificial cultivation and increasingly consumed in Japan and China owing to its high nutritional values and attractive taste (Smiderle et al., 2008). It has attracted considerable attention in the fields of its biological activities (Fu et al., 2002;Shi et al., 2012, Wasser, 2002. It was reported that the polysaccharides from the fruiting bodies of F. velutipes have been proved to be beneficial in immunomodulatory, antioxidant, antitumor and biological activity on hepatocytes (Leung et al., 1997;Ohkuma et al., 1983;Pang et al., 2007;. Several polysaccharides have been isolated from F. velutipes, and their pharmacological efficacies were investigated . For example, an antitumor polysaccharide with the structure of 1,3-glucan was isolated from cytoplasms of the fruiting bodies of F. velutipes (Ikekawa et al., 1982). An alkaline-soluble polysaccharide prepared from the cell wall of F. velutipes was reported to have immunomodulatory and antitumor activities against sarcoma SC180 (Leung et al., 1997). However, few studies on the bioactive of F. velutipes mycelia polysaccharides (FvP) were investigated. Furthermore, there are few investigations focusing on the effect of FvP on the proliferation of human hepatocellular cancer cells .Various methods are applied to increase the extraction efficiency of polysaccharides from mushrooms, including selection of proper sol...

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Isolation and characterization of a novel polysaccharide from the mucus of the loach, Misgurnus anguillicaudatus

Cited by 63 publications

References 17 publications

Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

Characterization and antitumor activity of pollen polysaccharide

In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes

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