Isolation and Characterization of a Major Antigenic Component of Malassezia globosa to IgE Antibodies in Sera of Patients with Atopic Dermatitis

Koyama, Tomoki; Kanbe, Toshio; Ishiguro, Akira; Kikuchi, Akihiko; Tomita, Yasushi

doi:10.1111/j.1348-0421.2000.tb02508.x

Cited by 20 publications

(9 citation statements)

References 18 publications

(17 reference statements)

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“…This hypothesis has been supported by the report that AD patients have positive reactions to Malassezia yeasts in patch tests [4]. In addition, specific Malassezia IgE antibodies were found in AD patients but not in healthy individuals [5,6,7,8,9,10]. …”

Section: Introductionsupporting

confidence: 67%

“…Moreover, the number of M. globosa on the face of AD females was clearly greater than that on healthy females analyzed by same method [27]. M. globosa is a typical antigen in AD [7]. On the other hand, the numbers of Malassezia species including M. globosa in AD males, and on the upper trunk of AD females, were not greater than those on healthy subjects [27].…”

Section: Discussionmentioning

confidence: 99%

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Cutaneous Malassezia Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

et al. 2010

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Background:Malassezia is a particularly important factor in the occurrence of atopic dermatitis (AD).Aim: The aim of this study was to quantitatively clarify the Malassezia species isolated from AD patients by gender, body part and analytical method in detail. Methods: The subjects were 20 AD males and 47 AD females. Samples were collected from lesion and nonlesion areas on the face and upper trunk of AD patients. Malassezia DNA was analyzed using a real-time PCR system. Results: The cutaneous Malassezia microbiota in AD patients differed by gender, body part and analytical method. Conclusions: The present results indicate the possibility that the influence of Malassezia antigens is different according to gender and body part.

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Section: Introductionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Cutaneous Malassezia Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

et al. 2010

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“…An antigen recently characterized from M. globosa with a molecular mass of 46 kDa was found to be a major antigen, reacting with 69% of sera from patients with AD (230). The antigen reacted with concanavalin A in lectin blots, indicating that it was a glycoprotein.…”

Section: Analysis Of the Antigens Present In Malasseziamentioning

confidence: 94%

Immunology of Diseases Associated withMalasseziaSpecies

2002

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SUMMARY Malassezia species are members of the human cutaneous commensal flora, in addition to causing a wide range of cutaneous and systemic diseases in suitably predisposed individuals. Studies examining cellular and humoral immune responses specific to Malassezia species in patients with Malassezia-associated diseases and healthy controls have generally been unable to define significant differences in their immune response. The use of varied antigenic preparations and strains from different Malassezia classifications may partly be responsible for this, although these problems can now be overcome by using techniques based on recent work defining some important antigens and also a new taxonomy for the genus. The finding that the genus Malassezia is immunomodulatory is important in understanding its ability to cause disease. Stimulation of the reticuloendothelial system and activation of the complement cascade contrasts with its ability to suppress cytokine release and downregulate phagocytic uptake and killing. The lipid-rich layer around the yeast appears to be pivotal in this alteration of phenotype. Defining the nonspecific immune response to Malassezia species and the way in which the organisms modulate it may well be the key to understanding how Malassezia species can exist as both commensals and pathogens.

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“…The major antigen for IgE antibodies in AD patients is a glycoprotein (Malg46b) from M. globosa (14,15). This paper describes differences between the IGS 1 genotypes of M. globosa colonizing the skin surface of both populations.…”

Section: Discussionmentioning

confidence: 99%

Sequence Diversity of the Intergenic Spacer Region of the rRNA Gene of Malassezia globosa Colonizing the Skin of Patients with Atopic Dermatitis and Healthy Individuals

Sugita¹,

Kodama

Saito

et al. 2003

J Clin Microbiol

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The lipophilic yeast Malassezia globosa is one of the major constituents of the mycoflora of the skin of patients with atopic dermatitis (AD). We compared the genotypes of M. globosa colonizing the skin surface of 32 AD patients and 20 healthy individuals for polymorphism of the intergenic spacer (IGS) 1 region of the rRNA gene. Sequence analysis demonstrated that M. globosa was divided into four major groups, which corresponded to the sources of the samples, on the phylogenetic tree. Of the four groups, two were from AD patients and one was from healthy subjects. The remaining group included samples from both AD patients and healthy subjects. In addition, the IGS 1 region of M. globosa contained short sequence repeats: (CT) n , and (GT) n . The number of sequence repeats also differed between the IGS 1 of M. globosa from AD patients and that from healthy subjects. These findings suggest that a specific genotype of M. globosa may play a significant role in AD, although M. globosa commonly colonizes both AD patients and healthy subjects.Malassezia species are lipophilic yeasts that are part of the normal human cutaneous commensal flora; they are isolated from sebaceous gland-rich areas of the skin, particularly on the chest, back, and head. They are also associated with several cutaneous diseases, including atopic dermatitis (AD), folliculitis, pityriasis versicolor, and seborrheic dermatitis (1, 7). In a taxonomic revision in 1996, the genus Malassezia was classified into seven different species: M. furfur, M. globosa, M. restricta, M. obtusa, M. pachydermatis, M. slooffiae, and M. sympodialis (9). Recently, we described an eighth species, M. dermatis, which was isolated from Japanese patients with AD (27). Since the taxonomic revision of the genus Malassezia, several studies have examined the distribution of the newly defined species of Malassezia on healthy human skin and lesions of skin diseases (2, 10, 21). However, culture media or sampling techniques often affect analyses of the Malassezia microflora. In a previous study, we used a nonculture method as an alternative to fungal culture to analyze the distribution of cutaneous Malassezia species (25). M. globosa and M. restricta were detected in approximately 90% of AD patients, and M. furfur and M. sympodialis were detected in approximately 40% of the subjects. In healthy subjects, M. globosa, M. restricta, and M. sympodialis were detected in approximately 40 to 60% of the subjects; M. furfur was found in only 4% of the subjects; and no other Malassezia species were detected. Therefore, these four species are common inhabitants of the skin of both AD patients and healthy individuals. In addition, while anti-Malassezia immunoglobulin E (IgE) antibody was detected in more than 90% of AD patients, no antibody was found in healthy subjects. Based on these results, M. globosa and M. restricta are thought to play a significant mycological role in AD. M. globosa is also part of the major microflora on the skin of healthy individuals. We used the intergenic space...

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Isolation and Characterization of a Major Antigenic Component of Malassezia globosa to IgE Antibodies in Sera of Patients with Atopic Dermatitis

Cited by 20 publications

References 18 publications

Cutaneous <i>Malassezia</i> Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

Cutaneous <i>Malassezia</i> Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

Immunology of Diseases Associated withMalasseziaSpecies

Sequence Diversity of the Intergenic Spacer Region of the rRNA Gene of Malassezia globosa Colonizing the Skin of Patients with Atopic Dermatitis and Healthy Individuals

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