Isolation and Characterization of a Mutant of Pseudomonas aureofaciens ATCC 15926 with an Increased Capacity for Synthesis of Pyrrolnitrin

Salcher, Olga; Lingens, Franz

doi:10.1099/00221287-118-2-509

Cited by 19 publications

(12 citation statements)

References 17 publications

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“…There is considerable evidence that pseudomonads produce a number of antimicrobial compounds (Howell & Stipanovie, 1980;Salcher & Lingens, 1980). In 1956 phenazine-1-carboxylic aeid, produced by a pseudomonad, was tested against a number of plant pathogens and shown to be toxic at concentrations of 75 A^g/ml (Haynes et al, 1956).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Brisbane

Rovira

1988

Plant Pathology

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The take-all fungus Gaei^niannomyces gratniuis var. tritici reduced the weight of wheat plants grown in tubes containing sterilized sand and plant nutrient solution. Fiuorescent pseudomonads, when added to the tubes, inhibited the take-ali fungus and increased piant weight. Iron (as FeNaEDTA) had no effect on the inhibition ofthe fungus by the pseudomonads. Some ofthe pseudomonads produced a compound in the wheat rhizosphere with a UV absorption peak at 365 nm, but the inhibition of G. graminis by pseudomonads was not proportionai to the UV absorbance of rhizosphere extracts. A yeiiow crystaiiine compound, absorbing at 365 nm, was extracted from broth cuitures and shown to be toxic to G. graminis under acid conditions. This compound is considered to be phenazine-i-carboxylic acid.

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Section: Discussionmentioning

confidence: 99%

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Brisbane

Rovira

1988

Plant Pathology

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“…The studies on anthranilic acid metabolism are part of our investigations on tryptophan metabolism in Pseudomonas pyrrocinia. Thus, O. Salcher and F. Lingens [13] reported that a mutant of Pseudomonas aureofaciens with a 30-fold increased rate of pyrrolnitrin production than the wild-type showed no anthranilate 2,3-dioxygenase activity and produced N-acetylaminophenol. Irregularities in tryptophan metabolism influence antibiotic production.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Formation of N-acetylaminophenol from anthranilic acid by Pseudomonas pyrrocinia ATCC15958

Lübbe¹

1982

FEMS Microbiology Letters

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“…Culture. The mutant strain ACN, which produces about 30-fold increased amounts of the antifungal antibiotic pyrrolnitrin, was obtained from the parent strain Pseudomonas aureofaciens ATCC 15926 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (14). Bacteria from which brornoperoxidase was isolated were grown for 4 days at 30°C with aeration (0.3 [vol/vol] per min) and stirring (150 rpm) in a 100-liter fermentor which was inoculated with 5 x 1-liter cultures from the late-exponential-growth phase.…”

Section: Methodsmentioning

confidence: 99%

“…SDS-polyacrylamide gels (C) were prepared by the method described by Weber and Osborn (20). The protein standards used were lysozyme (14,400), soybean trypsin inhibitor (21,500), carbonic anhydrase (31,000), ovalbumin (45,000), bovine serum albumin (66,200), and phosphorylase b (92,500). Spectroscopy.…”

Section: Methodsmentioning

confidence: 99%

Purification of bromoperoxidase from Pseudomonas aureofaciens

Pée¹,

Lingens²

1985

J Bacteriol

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A Bromoperoxidase has been isolated and purified from Pseudomonas aureofaciens ATCC 15926 mutant strain ACN. The purified enzyme was homogeneous as determined by polyacrylamide gel electrophoresis and ultracentrifugation. This bromoperoxidase can utilize bromide ions in the presence of hydrogen peroxide and a halogen acceptor for the catalytic formation of carbon-halogen bonds. The homogeneous enzyme also has peroxidase and catalase activity. Based on the results from gel filtration and ultracentrifugation, the molecular weight of this procaryotic bromoperoxidase is 155,000 to 158,000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows a single band having the mobility of a 77,000-molecular-weight species. We thus conclude that this bromoperoxidase exists in solution as a dimeric species. The heme prosthetic group of bromoperoxidase is ferriprotoporphyrin IX. The spectral properties of the native and reduced enzyme are reported. This bromoperoxidase is the first halogenating enzyme purified from procaryotic sources.

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Isolation and Characterization of a Mutant of Pseudomonas aureofaciens ATCC 15926 with an Increased Capacity for Synthesis of Pyrrolnitrin

Cited by 19 publications

References 17 publications

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Formation of N-acetylaminophenol from anthranilic acid by Pseudomonas pyrrocinia ATCC15958

Purification of bromoperoxidase from Pseudomonas aureofaciens

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