Purification of bromoperoxidase from Pseudomonas aureofaciens

Pée, K.-H. Van; Lingens, Franz

doi:10.1128/jb.161.3.1171-1175.1985

Cited by 60 publications

(12 citation statements)

References 16 publications

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“…We attribute the BrPO activity to the diatoms rather than bacteria because (1) the number of bacterial cells per diatom cell in the nonaxenic cultures was extremely low, especially in the cultures of polar species; (2) there was no difference in halogenation rates between P. glacialis cultures in exponential and stationary phase, whereas the later phase showed an increase in bacterial numbers; (3) calculated rates of release based on bacterial cell surface area are extremely high and unrealistic (.600 mmol cm 22 h 21 ); and (4) P. glacialis cultured in the presence of antibiotics reduced the number of bacteria by 80%, compared to a culture without antibiotics, with only a 22% reduction in PR halogenation. It has been reported that the BrPO activity (units mg 21 of BrPO enzyme) from the freshwater bacterium Pseudomonas aureofaciens was at least four orders of magnitude lower than the activity of algal BrPOs (van Pee and Lingens 1985). If the bacteria in the diatom cultures were contributing to the bromination of PR, it would likely be at a very low level compared to the diatom contribution.…”

Section: Discussionmentioning

confidence: 98%

Release of reactive bromine and iodine from diatoms and its possible role in halogen transfer in polar and tropical oceans

Hill

Manley

2009

Limnology & Oceanography

101

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An in situ incubation assay measuring the bromination and iodination of phenol red was developed to detect the release of reactive bromine and iodine (primarily hypobromous acid [HOBr] and hypoiodous acid [HOI], respectively) from a putative extracellular bromoperoxidase of marine diatoms. Six of 11 species showed significant release compared to controls. Polar species were particularly active, releasing 0.6-180 fmol HOBr cell 21 h 21 (0.04-1.8 mmol HOBr [mg total chlorophyll] 21 h 21 ; at the seawater bromide concentration, 840 mmol L 21 ) and 1.9-271 fmol HOI cell 21 h 21 (0.02-2.7 mmol HOI [mg total chlorophyll] 21 h 21 , at 100 mmol L 21 iodide). Porosira glacialis consistently showed the highest rates of release. Several temperate diatoms, including Achnanthes cf longipes, known to have a bromide-sensitive peroxidase involved in stalk formation, and warmwater species also showed the ability to release reactive bromine and iodine. This release was influenced by light, temperature, bromide (and iodide) concentration, H 2 O 2 concentration, and pH. The rate of HOBr release by polar diatoms was much greater than bromoform emissions measured by others from laboratory cultures and seaice algae in the field. This indicates that most of the HOBr released may react with dissolved organic matter (DOM) to form nonvolatile bromine organics. Some fraction of diatom-produced HOBr and HOI may also form volatile Br 2 and I 2 , which could transfer to the polar troposphere. The reaction of diatom-released reactive bromine and iodine with seawater DOM may represent the major mechanism in the formation of oceanic polybromo-and polyiodo-methanes.

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Section: Discussionmentioning

confidence: 98%

Release of reactive bromine and iodine from diatoms and its possible role in halogen transfer in polar and tropical oceans

Hill

Manley

2009

Limnology & Oceanography

101

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“…More recently, it has also been shown that species of diatoms release HOBr and IOBr, which may react with extracellular DOM [Hill and Manley, 2009]. Although most widespread in eukaryotic organisms, bromoperoxidases, although generally with lower halogenating activity, have also been identified in bacteria, e.g., in Pseudomonas aureofaciens [Van Pée and Lingens, 1985], and it has been shown that cyanobacteria may produce halocarbons [Johnson et al, 2011;Karlsson et al, 2008;Schall et al, 1996]. So even if algae were the most likely producers of the halocarbons measured, it cannot be excluded that bacteria were partly responsible for the halocarbon production.…”

Section: Polar Mixed Layer In the Makarov Basin Over The Lomonov Ridmentioning

confidence: 99%

Distribution, transport, and production of volatile halocarbons in the upper waters of the ice‐covered high Arctic Ocean

Theorin

Abrahamsson

2013

Global Biogeochemical Cycles

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[1] Volatile halogenated compounds (CHBr 3 , CH 2 Br 2 , CHBr 2 Cl, and CH 2 ClI) were measured in the water column and in sea ice brine across the Arctic Ocean, from Barrow, Alaska, to Svalbard, during the Beringia 2005 expedition (August-September) with RV/IB Oden. High concentrations of brominated compounds (up to 42 pmol kg À1 of bromoform) were found under multiyear ice in the surface waters over the Makarov Basin and the Lomonosov Ridge, near the North Pole. Even higher concentrations (bromoform up to 160 pmol kg À1 ) were found in sea ice brine. We propose that the high load of riverine dissolved organic matter that is transported in the Transpolar Drift is a main factor responsible for the high concentration of brominated volatile compounds found in sea ice brine and upper waters and that cycles of freezing and thawing during the transport enhance the transfer of halocarbons to the seawater. The iodinated compound (CH 2 ClI) showed a completely different distribution with highest concentrations in water of Pacific origin in the mixed layer and upper halocline of the northern Canada Basin and over the Alpha Ridge. In the southern Canada Basin, low concentrations of halocarbons were found in upper waters. Higher concentrations in water of Pacific origin, especially on the continental shelf, indicate production in the shelf regions, likely in the Chukchi Sea and the East Siberian Sea.

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“…This could be due to the fact that these enzymes are prone to fast enzyme inactivation, along with other known issues such as no substrate specificity and non-regioselectivity. [83][84][85]…”

Section: Heme-iron-dependent Haloperoxidases From Natural Product Patmentioning

confidence: 99%

Halogenases for biosynthetic pathway engineering: Toward new routes to naturals and non-naturals

2020

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Nature's repertoire of bio-halogenase enzymes is intriguing with halogenases from various natural product biosynthetic clusters that carry out site and region-specific halogenation of diverse bioactive precursors and molecules. Currently, we have a comprehensive catalogue of cryptic and non-cryptic halogenases that act on simple to complex aliphatic and aromatic molecular scaffolds. This will open up further synthetic and biosynthetic opportunities for C-H activation, ring formation and functionalization of different molecular structures. In fact, halogenases were exploited over the years for these potential applications, to replace traditional chemical halogenation chemistries toward creating economical and environmentally benign methodologies and also for biosynthetic pathways. This review will discuss our advances in utilizing biohalogenases to generate both in vivo and in vitro biosynthetic pathways; summarizing all naturals and non-naturals that are synthesized with a direct bio-halogenase incorporation.

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Purification of bromoperoxidase from Pseudomonas aureofaciens

Cited by 60 publications

References 16 publications

Release of reactive bromine and iodine from diatoms and its possible role in halogen transfer in polar and tropical oceans

Release of reactive bromine and iodine from diatoms and its possible role in halogen transfer in polar and tropical oceans

Distribution, transport, and production of volatile halocarbons in the upper waters of the ice‐covered high Arctic Ocean

Halogenases for biosynthetic pathway engineering: Toward new routes to naturals and non-naturals

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