Isolation and characterization of a desulforubidin-containing sulfate-reducing bacterium growing with glycolate

Friedrich, Michael W.; Schink, Bernhard

doi:10.1007/bf02529961

Cited by 23 publications

(16 citation statements)

References 44 publications

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“…Clone CARN-B1 fell within the Dsr-II cluster, and it is closely related to the laterally acquired dsrAB Firmicutes group (22,56). Cluster Dsr-III includes clones CARN-B4 (2%), CARN-A4 (40%), and CARN-B3 (23%) and is closely related to the glycolate oxidizer Desulfofustis glycolicus (16), belonging to the Desulfobulbaceae family. Cluster Dsr-IV encompasses clones CARN-B5 to -B10 (73% of the total clones analyzed from the CARN-B library).…”

Section: Resultsmentioning

confidence: 99%

Nested PCR and New Primers for Analysis of Sulfate-Reducing Bacteria in Low-Cell-Biomass Environments

Giloteaux

Goñi-Urriza

Duran

2010

Appl Environ Microbiol

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New primers were designed for the amplification of dsrAB genes by nested PCR to investigate the diversity of sulfate-reducing prokaryotes (SRP) in environments with low bacterial cell density. The success of the nested PCR for the determination of SRP diversity was estimated by terminal-restriction fragment length polymorphism analysis in the Reigous, a small creek at an inactive mine (Carnoulès, France), which constitutes an extreme acidic arsenic-rich environment. Nested PCR limits were evaluated in dsrAB-rich sediments, and this technique was compared to direct PCR using either known primers (DSR1F/DSR4R) or new primers (dsr619AF/dsr1905BR). The comparison of clone libraries revealed that, even if the levels of diversity observed were not identical, nested PCR did not reduce the diversity compared to that of direct DSR1F/DSR4R PCR. Clone sequences were affiliated mainly with the Desulfobacteraceae and Desulfohalobiaceae families. Many sequences (ϳ30%) were related to a deeply branching lineage unaffiliated with any cultured SRP. Although this dsrAB cluster was found in all libraries, the new primers better amplified this lineage, providing more information on this unknown bacterial group. Thanks to these new primers in nested PCR, the SRP community from Carnoulès could be characterized. Specific SRP populations were obtained according to environmental characteristics. Desulfomicrobiaceae-related sequences were recovered in samples with low pH, low levels of dissolved oxygen, and high As content, while sequences belonging to the deeply branching group were found in a less extreme sample. Furthermore, for the first time, dsrAB sequences related to the latter group were recovered from freshwater.

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Section: Resultsmentioning

confidence: 99%

Nested PCR and New Primers for Analysis of Sulfate-Reducing Bacteria in Low-Cell-Biomass Environments

Giloteaux

Goñi-Urriza

Duran

2010

Appl Environ Microbiol

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“…A desulforubidin preparation from cell-free extracts prepared by enrichment as described in Materials and methods exhibited an absorption spectrum with maxima at 545, 398 and 280 nm. The absorption ratio at 398 nm/454 nm (4.0) and at 398 nm/280 nm (0.65) indicated that this preparation was nearly pure desulforubidin (Friedrich and Schink 1995).…”

Section: Growth Physiology and Stochiometry Of Substrate Oxidationmentioning

confidence: 94%

“…Desulforubidin was enriched chromatographically and identified by absorption spectroscopy (after Friedrich and Schink 1995). A cytoplasmic cell fraction was loaded on a 5-ml High Trap Q anion-exchange column (Pharmacia, Freiburg, Germany) equilibrated with 20 mM Tris/HCl, pH 8.0 (buffer A).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Desulfotignum phosphitoxidans sp. nov., a new marine sulfate reducer that oxidizes phosphite to phosphate

Schink

Thiemann

Laue

et al. 2002

Archives of Microbiology

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105

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A new sulfate-reducing bacterium was isolated from marine sediment with phosphite as sole electron donor and CO 2 as the only carbon source. Strain FiPS-3 grew slowly, with doubling times of 3-4 days, and oxidized phosphite, hydrogen, formate, acetate, fumarate, pyruvate, glycine, glutamate, and other substrates nearly completely, with concomitant reduction of sulfate to sulfide. Acetate was formed as a side product to a small extent. Glucose, arabinose, and proline were partly oxidized and partly fermented to acetate plus propionate. Growth with phosphite, hydrogen, or formate was autotrophic. Also, in the presence of sulfate, CO dehydrogenase was present, and added acetate did not increase growth rates or growth yields. In the absence of sulfate, phosphite oxidation was coupled to homoacetogenic acetate formation, with growth yields similar to those in the presence of sulfate. Cells were small rods, 0.6-0.8×2-4 µm in size, and gram-negative, with a G+C content of 53.9 mol%. They contained desulforubidin, but no desulfoviridin. Based on sequence analysis of the 16S rRNA gene and the sulfite reductase genes dsrAB, strain FiPS-3 was found to be closely related to Desulfotignum balticum. However, physiological properties differed in many points from those of D. balticum. These findings justify the establishment of a new species, Desulfotignum phosphitoxidans.

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“…The molar cell yield for glycolate-grown cells of M. thermoacetica was similar to molar cell yields for D. glycolicus and a syntrophic homoacetogenic coculture containing S. glycolicus [18,20]. With D. glycolicus, the oxidation of glycolate to CO P is coupled to the reduction of sulfate to sul¢de, and, in the process, energy is conserved via electron transport phosphorylation [20].…”

Section: Discussionmentioning

confidence: 80%

Glycolate as a metabolic substrate for the acetogen Moorella thermoacetica

Seifritz

1999

FEMS Microbiology Letters

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Glycolate was growth supportive for Moorella thermoacetica ATCC 39073. Growth occurred in undefined and basal culture media containing 20 mM glycolate and was proportional to the concentration of glycolate (10^40 mM). Nitrate inhibited glycolate-dependent growth in the basal medium. Acetate and cell biomass were the major end products recovered from glycolate. The molar ratio (moles of glycolate required to synthesize a mole of acetate) averaged 1.4 and was in close agreement with the theoretical ratio of 1.3 for glycolate-derived acetogenesis. Glycolate-dependent growth yielded approximately 3-fold less biomass per pair of reducing equivalents utilized than did oxalate-or glyoxylate-dependent growth. z

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Isolation and characterization of a desulforubidin-containing sulfate-reducing bacterium growing with glycolate

Cited by 23 publications

References 44 publications

Nested PCR and New Primers for Analysis of Sulfate-Reducing Bacteria in Low-Cell-Biomass Environments

Nested PCR and New Primers for Analysis of Sulfate-Reducing Bacteria in Low-Cell-Biomass Environments

Desulfotignum phosphitoxidans sp. nov., a new marine sulfate reducer that oxidizes phosphite to phosphate

Glycolate as a metabolic substrate for the acetogen Moorella thermoacetica

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