Isolation and characterization of a high molecular weight glycoprotein from human blood platelets.

Lawler, J.; Slayter, Henry S.; Coligan, John E.

doi:10.1016/s0021-9258(17)34336-3

Cited by 385 publications

(61 citation statements)

References 17 publications

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“…Midkine (MDK, encoded by MDK ) is an enhancer of angiogenesis, and MDK expression has been shown to be positively correlated with vascular density in bladder tumors [ 61 ]. Thrombospondin 1 (THBS1, encoded by THBS1 ) is considered to be a potent endogenous inhibitor of angiogenesis through antagonization of vascular endothelial growth factor (VEGF) activity [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution

Gong

Yang

et al. 2021

Aging

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Human osteoblasts are multifunctional bone cells, which play essential roles in bone formation, angiogenesis regulation, as well as maintenance of hematopoiesis. However, the categorization of primary osteoblast subtypes in vivo in humans has not yet been achieved. Here, we used single-cell RNA sequencing (scRNA-seq) to perform a systematic cellular taxonomy dissection of freshly isolated human osteoblasts from one 31-year-old male with osteoarthritis and osteopenia after hip replacement. Based on the gene expression patterns and cell lineage reconstruction, we identified three distinct cell clusters including preosteoblasts, mature osteoblasts, and an undetermined rare osteoblast subpopulation. This novel subtype was found to be the major source of the nuclear receptor subfamily 4 group A member 1 and 2 (NR4A1 and NR4A2) in primary osteoblasts, and the expression of NR4A1 was confirmed by immunofluorescence staining on mouse osteoblasts in vivo . Trajectory inference analysis suggested that the undetermined cluster, together with the preosteoblasts, are involved in the regulation of osteoblastogenesis and also give rise to mature osteoblasts. Investigation of the biological processes and signaling pathways enriched in each subpopulation revealed that in addition to bone formation, preosteoblasts and undetermined osteoblasts may also regulate both angiogenesis and hemopoiesis. Finally, we demonstrated that there are systematic differences between the transcriptional profiles of human and mouse osteoblasts, highlighting the necessity for studying bone physiological processes in humans rather than solely relying on mouse models. Our findings provide novel insights into the cellular heterogeneity and potential biological functions of human primary osteoblasts at the single-cell level.

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Section: Resultsmentioning

confidence: 99%

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution

Gong

Yang

et al. 2021

Aging

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“…In comparison to TSP1, the first discovered TSP 39 with a readily available source for purification from platelets, functions of TSP4 are poorly studied. However, recent reports stressing the importance of TSP4 in cardiovascular system, 3‐17 in cancer, 14,15,18‐26 and in nervous system 27‐30,40 have attracted a lot of attention to this matricellular protein.…”

Section: Discussionmentioning

confidence: 99%

The P387 thrombospondin‐4 variant promotes accumulation of macrophages in atherosclerotic lesions

et al. 2020

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Thrombospondin‐4 (TSP4) is a pro‐angiogenic protein that has been implicated in tissue remodeling and local vascular inflammation. TSP4 and, in particular, its SNP variant, P387 TSP4, have been associated with cardiovascular disease. Macrophages are central to initiation and resolution of inflammation and development of atherosclerotic lesions, but the effects of the P387 TSP4 on macrophages remain essentially unknown. We examined the effects of the P387 TSP4 variant on macrophages in cell culture and in vivo in a murine model of atherosclerosis. Furthermore, the levels and distributions of the two TSP4 variants were assessed in human atherosclerotic arteries. In ApoE–/–/P387‐TSP4 knock‐in mice, lesions size measured by Oil Red O did not change, but the lesions accumulated more macrophages than lesions bearing A387 TSP4. The levels of inflammatory markers were increased in lesions of ApoE–/–/P387‐TSP4 knock‐in mice compared to ApoE–/– mice. Lesions in human arteries from individuals carrying the P387 variant had higher levels of TSP4 and higher macrophage accumulation. P387 TSP4 was more active in supporting adhesion of cultured human and mouse macrophages in experiments using recombinant TSP4 variants and in cells derived from P387‐TSP4 knock‐in mice. TSP4 supports the adhesion of macrophages and their accumulation in atherosclerotic lesions without changing the size of lesions. P387 TSP4 is more active in supporting these pro‐inflammatory events in the vascular wall, which may contribute to the increased association of P387 TSP4 with cardiovascular disease.

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“…Thrombocyte reactive protein-1 (TSP-1) is a stromal cell glycoprotein first identified in activated platelets (Lawler, 1978). It can be secreted and expressed in a variety of epithelial cells (Wight et al, 1985).…”

Section: Immunoregulatory Molecules and Inflammationmentioning

confidence: 99%

Recent Developments About the Pathogenesis of Dry Eye Disease: Based on Immune Inflammatory Mechanisms

Dong

et al. 2021

Front. Pharmacol.

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Dry eye disease is a common and frequently occurring ophthalmology with complex and diverse causes, and its incidence is on the upward trend. The pathogenesis of DED is still completely clear. However, the immune response based on inflammation has been recognized as the core basis of this disease. In this review, we will systematically review the previous research on the treatment of DED in immune inflammation, analyze the latest views and research hotspots, and provide reference for the prevention and treatment of DED.

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Isolation and characterization of a high molecular weight glycoprotein from human blood platelets.

Cited by 385 publications

References 17 publications

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution

The P387 thrombospondin‐4 variant promotes accumulation of macrophages in atherosclerotic lesions

Recent Developments About the Pathogenesis of Dry Eye Disease: Based on Immune Inflammatory Mechanisms

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