Isolation and characterization of a novel deoxynivalenol-transforming strain<i>Paradevosia shaoguanensis</i>DDB001 from wheat field soil

Wang, Yang; Zhang, Honghai; Zhao, Chen; Han, Yitao; Liu, Yuchun; Zhang, Xiaolin

doi:10.1111/lam.12790

Cited by 24 publications

(13 citation statements)

References 21 publications

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“…Many studies have pointed out that several microorganisms can convert DON to 3-keto-DON or 3- epi -DON. − However, the enzymes involved in epimerization are not well understood. In 2017, an aldo-keto reductase called AKR18A1 from Sphingomonas was identified which could oxidize DON into 3-keto-DON .…”

Section: Introductionmentioning

confidence: 99%

Structure–Function Analysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol Detoxification

Yang

Yan

et al. 2022

J. Agric. Food Chem.

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The pyrroloquinoline quinone (PQQ)-dependent dehydrogenase DepA detoxifies deoxynivalenol (DON) by converting the C3–OH into a keto group. Herein, two crystal structures of DepA and its complex with PQQ were determined, together with biochemical evidence confirming the interactions of DepA with PQQ and DON and revealing a unique tyrosine residue important for substrate selection. Furthermore, four loops over the active site essential for DepA activity were identified, of which three loops were stabilized by PQQ, and the fourth loop invisible in both structures was considered important for binding DON, together constituting a lid for the active site. Preliminary engineering of the loop showed its potential for enzyme improvement. This study provides structural insights into how a PQQ-dependent dehydrogenase is equipped with the function of DON conversion and for the first time shows the necessity of a lid structure for PQQ-dependent dehydrogenase activity, laying foundation for structure-based design to enhance catalysis efficiency.

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Section: Introductionmentioning

confidence: 99%

Structure–Function Analysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol Detoxification

Yang

Yan

et al. 2022

J. Agric. Food Chem.

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“…Paradevosia shaoguanensis . The Gram-negative P. shaoguanensis strain DDB001 isolated from the wheat field soil was reported to be able to completely degrade DON (200 μg/mL) already after an 8 h incubation at 30 °C in the MSM medium [ 124 ]. The LC-MS analysis of DON transformation products revealed the formation of 3-epi-DON.…”

Section: Biotransformation Of Deoxynivalenolmentioning

confidence: 99%

Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects

Statsyuk

Popletaeva

Щербакова

2023

BioTech

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Biological degradation of mycotoxins is a promising environmentally-friendly alternative to chemical and physical detoxification methods. To date, a lot of microorganisms able to degrade them have been described; however, the number of studies determining degradation mechanisms and irreversibility of transformation, identifying resulting metabolites, and evaluating in vivo efficiency and safety of such biodegradation is significantly lower. At the same time, these data are crucial for the evaluation of the potential of the practical application of such microorganisms as mycotoxin-decontaminating agents or sources of mycotoxin-degrading enzymes. To date, there are no published reviews, which would be focused only on mycotoxin-degrading microorganisms with the proved irreversible transformation of these compounds into less toxic compounds. In this review, the existing information about microorganisms able to efficiently transform the three most common fusariotoxins (zearalenone, deoxinyvalenol, and fumonisin B1) is presented with allowance for the data on the corresponding irreversible transformation pathways, produced metabolites, and/or toxicity reduction. The recent data on the enzymes responsible for the irreversible transformation of these fusariotoxins are also presented, and the promising future trends in the studies in this area are discussed.

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“…Massive screening techniques using diverse methods have been conducted to identify DON-degrading microbes, several of which have high DON conversion ability. Over the last three decades, DON-degrading strains have been identified among genera including Eubacterium (Fuchs et al, 2002), Eggerthella (Gao et al, 2018), Desulfitobacterium (He et al, 2020), Devosia (He et al, 2016;Wang et al, 2017Wang et al, , 2019, Sphingomonas (Ito et al, 2013;He et al, 2017), Nocardioides (Ikunaga et al, 2011), Marmoricola (Ito et al, 2012, Bacillus (Li et al, 2011), Pelagibacterium (Zhang et al, 2020), and Aspergillus (He et al, 2008;Jaqueline and Eliana, 2010;Jaqueline et al, 2011;Yang et al, 2017).…”

Section: Biodegradation Of Deoxynivalenol Bymentioning

confidence: 99%

Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

Zhang

Qin

et al. 2021

Front. Microbiol.

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Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-keto-DON was a necessary intermediate product during the conversion from DON to 3-epi-DON. Furthermore, the ZHH-013 strain could also utilize 3-epi-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-epi-DON elimination will be further studied, so that new enzymes for DON degradation can be identified.

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Isolation and characterization of a novel deoxynivalenol-transforming strainParadevosia shaoguanensisDDB001 from wheat field soil

Cited by 24 publications

References 21 publications

Structure–Function Analysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol Detoxification

Structure–Function Analysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol Detoxification

Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects

Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

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