Isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica)

Abstract

We report the isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica). These markers were tested in 24 individuals each collected from three distinct populations in Ichikai-machi, Tochigi Prefecture. The number of observed alleles per locus ranged from 3 to 24 across all populations, and the values of observed and expected heterozygosities ranged from 0.130 to 1 and from 0.125 to 0.941, respectively. These microsatellite loci will be useful for investigating the intraspecific genetic variation and population structure of this species.

Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace (Tribolodon hakonensis)

“…Although Hanzawa et al (1987) identified twelve mitochondrial DNA haplotypes among three locations on Honshu Island, this information is insufficient for investigating the intraspecific genetic diversity and detailed spatial structure of the populations within rural areas, prefectures, or regions. Therefore, we isolated and characterized 21 polymorphic microsatellite loci that could be useful for conservation genetic studies, following Koizumi et al (2007a, b;2009).…”