Isolation and characterisation of peroxiredoxin 6 promoter from sheep (Ovis aries)

Abstract

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein and a unique 1-Cys Prdx of the peroxiredoxin family. The expression and regulation of Prdx6 are implicated in numerous physiological and pathological processes. Material and Methods: Eight stepwise truncated DNA fragments obtained from the 5′-flank region of the Prdx6 gene were prepared and subcloned into the pSEAP2-Enhancer vectors. To investigate the transcriptional activity of the truncated DNA fragments, the recombinant plasmids were transfected into the COS-1 cells and the transcriptional activity was measured via assaying the expression of the reporter gene of the secreted alkaline phosphatase. Results: A 3.4 kb 5′-upstream flank region of the Prdx6 gene was cloned and sequenced. The region from -108 nt to -36 nt of the 5′-flanking region of the Prdx6 gene contained basal transcriptional activity. Conclusion: This result provides the basis for further studies on the gene regulation of the Prdx6-mediated biological processes and on screening for the transacting factors that interact with cis-acting elements of the Prdx6 gene promoter.

Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain

“…Peroxiredoxins is a Se-independent peroxidases superfamily and widely distributed in all phyla [7]. Prdx6, which shows GSH peroxidase (NSGPx) activity and phospholipase A2 (PLA2) activity [8] is the only 1-Cys member of the peroxiredoxin superfamily in mammalian [9]. The antioxidant activities of Prdx6 protect against oxidative stress [9, 10] and provide cell signalling [11].…”

Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6)

“…Similarly, another important property of Prdx6 is that it does not require thioredoxin to participate in the catalytic pathway, and glutathione (GSH) can act as a physiological reducing agent for Prdx6. The primary structure of Prdx6 consists of 224 amino acid residues, and its molecular weight ranges from 26 kDa to 29 kDa depending on the species (12).…”