Isolation and characterisation of new polymorphic microsatellite markers for the striped sea bream (Lithognathus mormyrus)

Sala-Bozano, Maria; Tsalavouta, Matina; Mariani, Stefano

doi:10.1007/s10592-008-9770-3

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Diluted PCR product (1 μL) was added to 9 μL of Hi‐Di Formamide and 0.2 μL 600LIZ® size standard. The sizing of PCR products (alleles) was performed on an ABI 3130xl genetic analyser using a protocol for multiplexed microsatellites adapted from Sala‐Bozano, Tsalavouta & Mariani (). Scoring was performed on GENEMAPPER 4.0 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Multiple paternity in the starry smooth-hound sharkMustelus asterias(Carcharhiniformes: Triakidae)

Farrell

O’Sullivan

Sacchi

et al. 2013

Biol J Linn Soc Lond

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Multiple paternity was investigated in the starry smooth-hound shark, Mustelus asterias Cloquet, 1821. Analysis of 12 pregnant females and their embryos, at four microsatellite loci, showed that at least 58% carried multiplysired litters. Paternal skew was observed, with one male siring most of the embryos within a litter, although no patterns of association were detected between the identity of the father and the size or the uterine position of the embryos. Sperm storage was observed in the oviducal glands of all twelve females. Results shed light on the complexity of reproductive strategies and selective processes in M. asterias.

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Section: Methodsmentioning

confidence: 99%

Multiple paternity in the starry smooth-hound sharkMustelus asterias(Carcharhiniformes: Triakidae)

Farrell

O’Sullivan

Sacchi

et al. 2013

Biol J Linn Soc Lond

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“…All individuals were polymerase chain reaction (PCR) amplified and genotyped at nine polymorphic microsatellite loci: Lm68, Lm72, Lm19, Lm86, Lm12, Ad05, Ad66, SaL15 and Sal19 (Brown et al ., 2005; Franch et al ., 2006). Samples were processed in two multiplex reactions (see Sala‐Bozano, Tsalavouta & Mariani, 2008 for details). Genotyping of individuals was performed by allele sizing on an ABI 3130 xl Genetic Analyser (Applied Biosystems) using forward primers labelled with NED, PET, FAM and VIC dyes and an internal size standard labelled with LIZ 600 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Impact of a mouth parasite in a marine fish differs between geographical areas

Sala-Bozano

Oosterhout

Mariani

2012

Biological Journal of the Linnean Society

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Considerable variation exists in parasite virulence and host tolerance which may have a genetic and/or environmental basis. In this article, we study the effects of a striking, mouth-dwelling, blood-feeding isopod parasite (Ceratothoa italica) on the life history and physiological condition of two Mediterranean populations of the coastal fish, Lithognathus mormyrus. The growth and hepatosomatic index (HSI) of fish in a heavily human-exploited population were severely impacted by this parasite, whereas C. italica showed negligible virulence in fish close to a marine protected area. In particular, for HSI, the parasite load explained 34.4% of the variation in HSI in the exploited population, whereas there was no significant relationship (0.3%) between parasite load and HSI for fish in the marine protected area. Both host and parasite populations were not differentiated for neutral genetic variation and were likely to exchange migrants. We discuss the role of local genetic adaptation and phenotypic plasticity, and how deteriorated environmental conditions with significant fishing pressure can exacerbate the effects of parasitism. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 105, 842–852

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“…2009). Samples were processed in two multiplex reactions following the protocol in Sala‐Bozano et al. (2009).…”

Section: Methodsmentioning

confidence: 99%

Contrasting signals from multiple markers illuminate population connectivity in a marine fish

2009

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Recent advances in molecular biology and bioinformatics have helped to unveil striking and previously unrecognized patterns of geographic genetic structure in marine populations. Largely driven by the pressing needs of fisheries management and conservation, studies on marine fish populations have played a pivotal role in testing the efficiency of a range of approaches to explore connectivity and dispersal at sea. Here, we employed nuclear and mitochondrial DNA markers and parasitic infestations to examine the nature and patterns of population structure in a warm-temperate coastal marine teleost across major putative biogeographic barriers in the Mediterranean Sea and Eastern Atlantic Ocean. We detected deep genetic divergence between mitochondrial lineages, likely caused by dramatic climatic and geological transformations before and during the Pleistocene. Such long-diverged lineages later came into secondary contact and can now be found in sympatry. More importantly, microsatellite data revealed that these lineages, after millions of years of independent evolution, now interbreed extensively. By combining genetic and parasite data, we were able to identify at least five independent demographic units. While the different genetic and parasite-based methods produce notably contrasting signals and may complicate the reconstruction of connectivity dynamics, we show that by tailoring the correct interpretation to each of the descriptors used, it is possible to achieve a deeper understanding of the micro-evolutionary process and, consequently, resolve population structure.

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Isolation and characterisation of new polymorphic microsatellite markers for the striped sea bream (Lithognathus mormyrus)

Cited by 5 publications

References 15 publications

Multiple paternity in the starry smooth-hound sharkMustelus asterias(Carcharhiniformes: Triakidae)

Multiple paternity in the starry smooth-hound sharkMustelus asterias(Carcharhiniformes: Triakidae)

Impact of a mouth parasite in a marine fish differs between geographical areas

Contrasting signals from multiple markers illuminate population connectivity in a marine fish

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