Isolation and characterisation of dog uropathogenic Escherichia coli strains and their fimbriae

Garcia, Elisa; Bergmans, Hans; Bosch, J. F. van den; Ørskov, Ida; Zeijst, Bernard A.M. van der; Gaastra, Wim

doi:10.1007/bf00419202

Cited by 40 publications

(47 citation statements)

References 34 publications

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“…However, the limited sample size precludes firm conclusions. It must be remembered that for some time after its discovery, papG allele III was presumed to be specific to dogs (3,6,40,53), whereas it is now known to be common also among human isolates from diverse clinical syndromes (1,14,16,21,26,42). Additional investigation is needed to define the epidemiological associations and host range of papG allele IЈ.…”

Section: Discussionmentioning

confidence: 99%

Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG

et al. 2001

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P fimbriae of extraintestinal pathogenic Escherichia coli mediate digalactoside-specific adherence via the tip adhesin molecule PapG, which occurs in three known variants (I to III), which are encoded by the corresponding three alleles of papG. In the present study, newly discovered variants of papG allele I and the respective wild-type source strains were characterized. One of the new papG allele I variants conferred a unique agglutination phenotype that combined the phenotypes associated with papG alleles I, II, and III. Comparative hydrophilicity analysis of predicted PapG peptides revealed regions that might explain the observed phenotypic similarities and differences between the PapG variants. The new papG allele I variants occurred either as the sole papG allele or together with both papG alleles II and III, rather than with only papG allele III, as in archetypal strains J96 and CP9. They also occurred in the absence of the usual F13 papA allele. One of the new papG allele I variants occurred in a serogroup O6 strain that, according to random amplified polymorphic DNA analysis, was phylogenetically distant from the "J96-like" clonal group of E. coli O4:H5, which includes all previously identified examples of papG allele I. Cluster analysis of nucleotide and predicted peptide sequences suggested that papG allele I represents the earliest evolutionary branch from a common papG ancestor. These results demonstrate unexpected diversity within papG allele I and, together with previous findings, suggest that the J96-like clonal group of E. coli O4:H5 may represent the original source of papG within the species.

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Section: Discussionmentioning

confidence: 99%

Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG

et al. 2001

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“…P fimbriae have been grouped into at least 11 serotypes, designated F7, to Fl6, based on differences in the major antigenic structural PapA-related proteins (Hacker, 1992). P fimbriae have been associated with E. coli strains causing UTI in humans (Johnson, 1991) and dogs (Garcia et a/., 1988), and causing septicaemia in pigs (Harel e t a/,, 1993). They are also expressed by certain E. coli strains implicated in colisepticaemia and airsacculitis of poultry (Dozois et a/., 1992;van den Bosch e t al., 1993;Pourbakhsh & Fairbrother, 1994).…”

Section: Introductionmentioning

confidence: 99%

P-fimbriae-producing septicaemic Escherichia coli from poultry possess fel-related gene clusters whereas pap-hybridizing P-fimbriae-negative strains have partial or divergent P fimbrial gene clusters

Dozois

Harel²,

Fairbrother³

1996

Microbiology

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CP5000, Sa i nt-H yaci nt he, Quebec, Canada J2S 7C6The organization of P f imbrial gene clusters of 13 papC-hybridizing Escherichia coli strains isolated from poultry with colisepticaemia, five P-fimbriaeexpressing (P-positive) and eight P-fimbriae-non-expressing (P-negative), were examined by PCR and by Southern blot hybridization using primers or gene probes specific to the 1 , B, A, C or G genes. The absence of P fimbrial expression was associated with lack of PCR amplification of one or more of these genes, most commonly the I gene. Restriction endonuclease EcoRI, BamHl or Pstl digests of genomic DNA from all strains hybridized with each of the gene probes and demonstrated polymorphisms between P-positive and P-negative strains. Pstl digests of DNA from 12 of the 13 strains, when hybridized with the A gene probe, demonstrated a 01 kb fragment specific to the felA gene which encodes the major structural protein of F11 fimbriae. Hence, only the Ppositive strains contained complete copies of fel-related gene clusters. In contrast, most of the pap-hybridizing P-negative strains contained partial or divergent P fimbrial gene clusters, which explains the lack of P fimbrial expression by these strains.

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“…Several cross-sectional surveys have demonstrated similarities among clinical or fecal E. coli isolates from humans, dogs, and cats with respect to genomic background and virulence-associated accessory traits (virulence factors [VFs]), particularly within serogroups O6 and O4, suggesting possible zoonotic (whether animal-to-human or human-to-animal) transmission (3,5,6,9,11,13,15,16,20,26,(30)(31)(32)(33). Consistent with this possibility, in two longitudinal surveillance studies involving the E. coli flora of human household members and their canine or feline pets, pets were found to be intermittently colonized with the same virulent-appearing E. coli clones that colonized multiple humans and caused acute cystitis in the women (8,22).However, these studies examined only modest numbers of isolates and accessory traits and/or relied on somewhat imprecise phylogenetic methods such as multilocus enzyme electrophoresis, outer membrane protein profiling, or random amplified polymorphic DNA analysis (3,5,6,8,9,11,13,15,16,20,22,26,(30)(31)(32)(33). Accordingly, they leave uncertainty as to the extent of commonality among human-and pet-derived E. coli isolates.…”

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confidence: 99%

Virulence Genotypes and Phylogenetic Background ofEscherichia coliSerogroup O6 Isolates from Humans, Dogs, and Cats

Johnson¹,

Johnston²,

Clabots³

et al. 2008

J Clin Microbiol

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Molecular evidence is limited for the hypothesis that humans, dogs, and cats can become colonized and infected with similar virulent Escherichia coli strains. To further assess this possibility, archived E. coli O6 isolates (n ‫؍‬ 130) from humans (n ‫؍‬ 55), dogs (n ‫؍‬ 59), and cats (n ‫؍‬ 16), representing the three main H (flagellar) types within serogroup O6 (H1, H7, and H31), were analyzed, along with selected reference strains. Isolates underwent PCR-based phylotyping, multilocus sequence typing, PCR-based detection of 55 virulenceassociated genes, and XbaI pulsed-field gel electrophoresis (PFGE) profiling. Three major sequence types (STs), which corresponded closely with H types, accounted for 99% of the 130 O6 isolates. Each ST included human, dog, and cat isolates; two included reference pyelonephritis isolates CFT073 (O6:K2:H1) and 536 (O6:K15:H31). Virulence genotypes overlapped considerably among host species, despite statistically significant differences between human and pet isolates. Several human and dog isolates from ST127 (O6:H31) exhibited identical virulence genotypes and highly similar PFGE profiles, consistent with cross-species exchange of specific E. coli clones. In conclusion, the close similarity in the genomic backbone and virulence genotype between certain human-and animal-source E. coli isolates within serogroup O6 supports the hypothesis of zoonotic potential.Escherichia coli is a major cause of urinary tract infections (UTI) and other extraintestinal infections in humans, dogs, and cats (1,19,25). Several cross-sectional surveys have demonstrated similarities among clinical or fecal E. coli isolates from humans, dogs, and cats with respect to genomic background and virulence-associated accessory traits (virulence factors [VFs]), particularly within serogroups O6 and O4, suggesting possible zoonotic (whether animal-to-human or human-to-animal) transmission (3,5,6,9,11,13,15,16,20,26,(30)(31)(32)(33). Consistent with this possibility, in two longitudinal surveillance studies involving the E. coli flora of human household members and their canine or feline pets, pets were found to be intermittently colonized with the same virulent-appearing E. coli clones that colonized multiple humans and caused acute cystitis in the women (8,22).However, these studies examined only modest numbers of isolates and accessory traits and/or relied on somewhat imprecise phylogenetic methods such as multilocus enzyme electrophoresis, outer membrane protein profiling, or random amplified polymorphic DNA analysis (3,5,6,8,9,11,13,15,16,20,22,26,(30)(31)(32)(33). Accordingly, they leave uncertainty as to the extent of commonality among human-and pet-derived E. coli isolates.To examine more rigorously the question of human-pet commonality with respect to extraintestinal pathogenic E. coli (ExPEC) clones, we used contemporary molecular methods to characterize a large collection of archival E. coli isolates of serotypes O6:H1, O6:H7, and O6:H31 from humans, dogs, and cats, obtained from the E. coli Reference Ce...

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Isolation and characterisation of dog uropathogenic Escherichia coli strains and their fimbriae

Cited by 40 publications

References 34 publications

Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG

Novel Molecular Variants of Allele I of the Escherichia coli P Fimbrial Adhesin Gene papG

P-fimbriae-producing septicaemic Escherichia coli from poultry possess fel-related gene clusters whereas pap-hybridizing P-fimbriae-negative strains have partial or divergent P fimbrial gene clusters

Virulence Genotypes and Phylogenetic Background ofEscherichia coliSerogroup O6 Isolates from Humans, Dogs, and Cats

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