P-fimbriae-producing septicaemic Escherichia coli from poultry possess fel-related gene clusters whereas pap-hybridizing P-fimbriae-negative strains have partial or divergent P fimbrial gene clusters

Dozois, Charles M.; Harel, Josée; Fairbrother, John M.

doi:10.1099/13500872-142-10-2759

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…The presence of different toxins has also been reported but with different prevalences (Blanco et al, 1997;Parreira & Gyles, 2002;Reingold et al, 1999). Gene inactivation studies confirmed a role in the pathogenicity of APEC for the F1 (type 1) and P fimbrial adhesins (Dozois et al, 1992(Dozois et al, , 1996La Ragione et al, 2000a, b;Marc et al, 1998;Pourbakhsh et al, 1997), for the K1-antigen mediating resistance to phagocytosis (Mellata et al, 2003a), for the aerobactin high-affinity iron transport system (Lafont et al, 1987) and for the temperature-sensitive haemagglutinin Tsh (Dozois et al, 2000;Provence & Curtiss, 1994). Recently, Parreira & Gyles (2003) showed that a vacuolating toxin, Vat, was involved in the virulence of an APEC isolate.…”

Section: Introductionmentioning

confidence: 83%

“…The presence of several putative virulence genes has been positively linked to the pathogenicity of APEC strains. Indeed, several adhesins and the tsh gene are more frequently found in pathogenic isolates (Dozois et al, 1992(Dozois et al, , 1996Stordeur et al, 2002). The presence of different toxins has also been reported but with different prevalences (Blanco et al, 1997;Parreira & Gyles, 2002;Reingold et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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ibeA, a virulence factor of avian pathogenic Escherichia coli

et al. 2005

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The presence of ibeA, a gene encoding a known virulence factor of Escherichia coli strains responsible for neonatal meningitis in humans, was investigated in the genome of 213 avian pathogenic E. coli (APEC) strains and 55 non-pathogenic E. coli strains of avian origin. Fifty-three strains were found to be ibeA + , all of which belonged to the APEC group. The ibeA gene is therefore positively linked to the pathogenicity of strains (P<0?0001). Analysis of the serogroup of strains revealed a positive association of ibeA with serogroups O18, O88 and O2. On the contrary, only 1/59 O78 strains are ibeA + , indicating a negative association of ibeA with this serogroup (P<0?0001). The role of ibeA in the virulence of the APEC strain BEN 2908 was investigated by constructing an ibeA mutant. Challenge assays on 3-week-old chickens showed a reduced virulence for the ibeA mutant. Furthermore, the APEC strain BEN 2908 was able to invade brain microvascular epithelial cells, this invasion being significantly reduced upon inactivation of ibeA. Altogether, these results suggest a role of ibeA in the pathogenicity of some APEC strains and confirm the close relationship between APEC and other human extraintestinal pathogenic E. coli isolates.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

ibeA, a virulence factor of avian pathogenic Escherichia coli

et al. 2005

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“…Among the 11 PapA variants tested, only the F11 papA allele was detected in all 18 strains. In addition to the F165 fimbria, which was detected in the strains tested in this study, which were isolated from pigs and calves with septicemia, interestingly, F11 is the predominant serotype of P fimbriae expressed by E. coli strains implicated in avian colisepticemia (16). This suggests that F11 fimbriae are frequently expressed by pathogenic E. coli strains isolated from animals with septicemia.…”

Section: Discussionmentioning

confidence: 81%

Presence and Characterization of Extraintestinal Pathogenic Escherichia coli Virulence Genes in F165-Positive E. coli Strains Isolated from Diseased Calves and Pigs

Dezfulian¹,

Batisson²,

Fairbrother³

et al. 2003

J Clin Microbiol

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The virulence genotype profile and presence of a pathogenicity island(s) (PAI) were studied in 18 strains of F165-positive Escherichia coli originally isolated from diseased calves or piglets. On the basis of their adhesion phenotypes and genotypes, these extraintestinal pathogenic strains were classified into three groups. The F165 fimbrial complex consists of at least two serologically and genetically distinct fimbriae: F165 1 and F165 2 . F165 1 is encoded by the foo operon (pap-like), and F165 2 is encoded by fot (sfa related). Strains in group 1 were foo and fot positive, strains in group 2 were foo and afa positive, and strains in group 3 were foo positive only. The strains were tested for the presence of virulence genes found mainly in extraintestinal pathogenic E. coli (ExPEC) strains. Although all the strains were positive for the papA variant encoding F11 fimbriae incD, traT, and papC, the prevalence of virulence genes commonly found in PAIs associated with ExPEC strains was highly variable, with strains of group 2 harboring most of the virulence genes tested. papG allele III was detected in all strains in group 1 and in one strain in group 3. All other strains were negative for the known alleles encoding PapG adhesins. The association of virulence genes with tRNA genes was characterized in these strains by using pulsed-field gel electrophoresis and DNA hybridization. The insertion site of the foo operon was found at the pheU tRNA locus in 16 of the 18 strains and at the selC tRNA locus in the other 2 strains. Furthermore, 8 of the 18 strains harbored a high-pathogenicity island which was inserted in either the asnT or the asnV/U tRNA locus. These results suggest the presence of one or more PAIs in septicemic strains from animals and the association of the foo operon with at least one of these islands. F165-positive strains share certain virulence traits with ExPEC, and most of them are pathogenic in piglets, as tested in experimental infections.Escherichia coli is a frequent cause of intestinal and extraintestinal diseases in humans and animals. Typical extraintestinal infections include urinary tract infections, newborn meningitis, polyserositis, and septicemia. All these groups of pathogenic E. coli strains have been called extraintestinal pathogenic E. coli (ExPEC). The recognized virulence factors of ExPEC include diverse adhesins (e.g., P fimbriae, S/F1C fimbriae, F165 fimbriae, Afa/Dr adhesins, and type 1 fimbriae), toxins (e.g., hemolysin, cytotoxic necrotizing factor, and cytolethal distending toxin), surface antigens (e.g., group II and group III capsules and lipopolysaccharide), invasins (e.g., an invasin responsible for invasion of brain endothelium [IbeA, also called Ibe10]), iron uptake systems (e.g., the aerobactin system), and secretion systems (e.g., type III secretion systems). These virulence factors facilitate colonization and invasion of the host, avoidance or disruption of host defense mechanisms, injury to host tissues, and/or stimulation of a noxious host inflammatory response (...

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“…Previous efforts to exploit DNA sequence polymorphisms for typing of papA variants by restriction endonuclease analysis (19) or with a battery of oligonucleotide probes (13) were handicapped by the unavailability of sequence data for the full range of papA alleles. Full-length sequencing of papA from wild-type strains, although highly informative and requiring no prior knowledge of existing papA sequences (8), is impractical for large-scale screening.…”

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confidence: 99%

Analysis of the F Antigen-Specific papA Alleles of Extraintestinal Pathogenic Escherichia coli Using a Novel Multiplex PCR-Based Assay

et al. 2000

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Polymorphisms in PapA, the major structural subunit and antigenic determinant of P fimbriae of extraintestinal pathogenic Escherichia coli, are of considerable epidemiological, phylogenetic, and immunotherapeutic importance. However, to date, no method other than DNA sequencing has been generally available for their detection. In the present study, we developed and rigorously validated a novel PCR-based assay for the 11 recognized variants of papA and then used the new assay to assess the prevalence, phylogenetic distribution, and bacteriological associations of the papA alleles among 75 E. coli isolates from patients with urosepsis. In comparison with conventional F serotyping, the assay was extremely sensitive and specific, evidence that papA sequences are highly conserved within each of the traditionally recognized F serotypes despite the diversity observed among F types. In certain strains, the assay detected serologically occult copies of papA, of which some were shown to represent false-negative serological results and others were shown to represent the presence of nonfunctional pap fragments. Among the urosepsis isolates, the assay revealed considerable segregation of papA alleles according to O:K:H serotype, consistent with vertical transmission within clones, but with exceptions which strongly suggested horizontal transfer of papA alleles between lineages. Sequencing of papA from two strains that were papA positive by probe and PCR but F negative in the new PCR assay led to the discovery of two novel papA variants, one of which was actually more prevalent among the urosepsis isolates than were several of the known papA alleles. These findings provide novel insights into the papA alleles of extraintestinal pathogenic E. coli and indicate that the F PCR assay represents a versatile new molecular tool for epidemiological and phylogenetic investigations which should make rapid, specific detection of papA alleles available to any laboratory with PCR capability.

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P-fimbriae-producing septicaemic Escherichia coli from poultry possess fel-related gene clusters whereas pap-hybridizing P-fimbriae-negative strains have partial or divergent P fimbrial gene clusters

Cited by 13 publications

References 29 publications

ibeA, a virulence factor of avian pathogenic Escherichia coli

ibeA, a virulence factor of avian pathogenic Escherichia coli

Presence and Characterization of Extraintestinal Pathogenic Escherichia coli Virulence Genes in F165-Positive E. coli Strains Isolated from Diseased Calves and Pigs

Analysis of the F Antigen-Specific papA Alleles of Extraintestinal Pathogenic Escherichia coli Using a Novel Multiplex PCR-Based Assay

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