Isolation and Biological Activity of β-Sitosterol and Stigmasterol from the Roots of Indigofera heterantha

Abstract

In the ongoing phytochemical studies the roots of Medicinal plant known as Indigofera heterantha were investigated phytochemicaly in order to explore its medicinal potential. The ethyl acetate fraction was subjected to column chromatography which resulted in the isolation and purification of two new source compounds known as β-sitosterol (1) and Stigmasterol (2). The structures of these compounds were established by using Mass Spectrometry. The structures of these compounds were further authenticated by comparison with the literature data. The isolated pure compounds were screened for antidiabetic and membrane stabilizing activities. The antidiabetic activity of β-sitosterol (1) was measured on the basis of the glucose uptake in yeast cells. The results obtained revealed that β-sitosterol (1) exhibited significantly antidiabetic activity at all glucose concentrations with minimum increase 66.48% at 10µg/ml and maximum increase 74.46% at 80μg/ml glucose concentration. The Stigmasterol (2) showed good membrane stabilizing activity in HRBC, the minimum stabilization 62.74% at 10μg/ml and maximum stabilization 65.19% at 40μg/ml. These results showed that this plant is very important from medical point of view and it needs further phytochemical investigation to explore its hidden medicinal potential.

Oleanane and Stigmasterol-Type Triterpenoid Derivatives from the Stem Bark of Albizia gummifera and Their Antibacterial Activities

“…It is worth to highlight that stigmasterol has been reported to inhibit several proinflammatory and matrix degradation mediators typically involved in human osteoarthritis-(OA-) induced cartilage degradation, at least in part through the inhibition of the NF-κB pathway [21]. It also showed good membrane stabilizing activity in HRBC with minimum stabilization 62.74% at 10 μg/mL and maximum stabilization 65.19% at 40 μg/mL [22].…”

Phytochemical, antimicrobial and cytotoxicity screening of ethanol extract of <i>Acacia ehrenbergiana</i> Hayne grown in Jazan Region of Saudi Arabia

“…Using GC-MS, 56.09 % phytoconstituents from ethanol extract were identified. Components such as dasycarpidan-1-methanol acetate (ester), β-sitosterol, 1-monolinoleoylglycerol, squalene, octacosanol, α-amyrin and lupeol have been previously reported to have antibacterial, antifungal, anti-inflammatory, anti-pyretic, antidiabetic and anticancer activities [20][21][22].…”

Natural Compounds, Pharmacological Activities, and Conservation of Eria (Orchidaceae)

“…A biologically active β-sitosterol has been isolated from E. marginata [11]. Sitosterol is recognised to possess a wide range of biological activities including antibacterial, anticancer, antidiabetic, and antiinflammatory activities [54][55][56][57].…”