Isolation and anti-inflammatory activity of colchicinoids from<i>Gloriosa superba</i>seeds

Joshi, Charu; Ekambaram, Sanmuga Priya; Mathela, C. S.

doi:10.3109/13880200903081770

Cited by 31 publications

(12 citation statements)

References 29 publications

(25 reference statements)

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“…The present study thus confirmed that G. superba could be used as potent antiinflammatory agent. The results are found to be in correlation with the previous studies which were done in carrageenan induced animal models (Abhishek et al, 2011;Jomy et al, 2009;Joshi et al, 2010).…”

Section: Discussionsupporting

confidence: 91%

Anti inflammatory properties of the root tubers of Gloriosa superba and its conservation through micropropagation

Nikhila¹,

Sangeetha²,

Swapna³

2015

J. Med. Plants Res.

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Gloriosa superba L. (Liliaceae) is an important medicinal herb of Asia and Africa. The plant is used to cure ulcers, piles, cancer, gout, scrofula and act as abortifacient, anthelmintic, antipyretic and antiinflammatory drug. The main aim of the proposed work is to evaluate the anti-inflammatory activity of G. superba and also conserve the same plant through the in vitro propagation. The anti-inflammatory activity was evaluated by cycloxygenase inhibition assay and 5-lipoxygenase inhibition assay. In the cycloxygenase inhibition assay, the percentage inhibition were found to be 3.38%, 26.27%, 43.22% for sample concentration 100µg/ml, 500µg/ml, 1000µg/ml respectively and in the lipoxygenase inhibition assay percentage inhibition were found to be 49.23%, 76.92%, 84.61% for sample concentration 100µg/ml, 500µg/ml, 1000µg/ml respectively. In lipoxygenase inhibition assay the methanol extract of G. superba tuber showed close percentage inhibition with that of the standard aspirin. The present study thus confirmed that methanol extract of root tubers of G. superba possessed good anti-inflammatory activity. At present, the plant is on the way of extinction due to its misuse, over exploitation and unscientific collection. Micro propagation is an important method to conserve this highly antiinflammatory medicinal plant. In vitro studies of G. superba includes induction of callusing and organogenesis, using various explants. The results indicated that medium supplemented (MS) with auxin (NAA) (0.15 mg/l) and Benzylamino purine (BAP) (0.25 mg/l) induced callusing, with 2,4-D (0.5 mg/l) and Kinetin (0.25 mg/l) induced somatic embryogenesis, NAA (0. 5mg/l) and BAP (0.25 mg/l) promoted the formation of the maximum number of shooting and with NAA (0.25 mg/l) and BAP (0.15 mg/l) rooting was induced. Micro propagation will be helpful for the conservation and maximum utilization of this plant with high antiinflamatory potential along with the identification and isolation of useful bioactive molecules.

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Section: Discussionsupporting

confidence: 91%

Anti inflammatory properties of the root tubers of Gloriosa superba and its conservation through micropropagation

Nikhila¹,

Sangeetha²,

Swapna³

2015

J. Med. Plants Res.

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“…The tubers contain colchicines, benzoic and salicylic acid, sterols and resinous substances -colchicines, 1,2-didemethyl colchicines, 2,3-didemethylcolchicine, N-deacetylcolchicines, colchicoside, gloriosine, tannins and superbine (Capraro and Brossi, 1984). Joshi et al (2010) reported isolation of colchicine, 2-demethylcolchicine, 3-demethylcolchicine and N-formyl-N-deacetylcolchicine from the GS seeds. The active principles of leaves are superbine, colchicine, gloriosine, gloriosol, phytosterols and stigmasterol (Kayode and Kayode, 2008).…”

Section: Gloriosa Superba (Family: Liliaceae)mentioning

confidence: 99%

Potential antifertility agents from plants: A comprehensive review

Kumar

Prakash

2012

Journal of Ethnopharmacology

116

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“…Instead, nonaqueous solutions have been applied to separate, e.g. forskolin using MeOH-MeCN [19], colchicinoids using EtOH [20] and limonoids using CHCl 3 -MeOH [14]. In comparison with aqueous solution, non-aqueous solution has some advantages, e.g.…”

Section: Selection Of the Solvent System And Elution Programmentioning

confidence: 99%

Single-Step Preparative Extraction of Artemisinin from Artemisia annua by Charcoal Column Chromatography

Luo

Kong

2011

Chromatographia

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Activated charcoal column chromatography was successfully applied for preparative extraction of artemisinin with high-purity from Artemisia annua L. in one single run for the first time in this study. The adsorption kinetics study showed that powdery activated charcoal had high adsorption speed for artemisinin. The experimental adsorption data fitted well using the Langmuir adsorption model. The optimal parameters for the extraction of artemisinin were obtained using a column packed with activated charcoal: the loading amount, 1 g extract/ 5 g activated charcoal; the elution mode, CH 2 Cl 2 -MeOH (2:5, v/v) for 5 BV (bed volume) after the removal of some impurities with CH 2 Cl 2 -MeOH (1:10, v/v). With this optimized condition, artemisinin was finally obtained with purity at 95.2% and the recovery at 72.3%. The developed technique would provide a feasible large-scale method for the extraction of artemisinin in pharmaceutical industry.

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Isolation and anti-inflammatory activity of colchicinoids fromGloriosa superbaseeds

Cited by 31 publications

References 29 publications

Anti inflammatory properties of the root tubers of Gloriosa superba and its conservation through micropropagation

Anti inflammatory properties of the root tubers of Gloriosa superba and its conservation through micropropagation

Potential antifertility agents from plants: A comprehensive review

Single-Step Preparative Extraction of Artemisinin from Artemisia annua by Charcoal Column Chromatography

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