Anti inflammatory properties of the root tubers of Gloriosa superba and its conservation through micropropagation

Abstract

Gloriosa superba L. (Liliaceae) is an important medicinal herb of Asia and Africa. The plant is used to cure ulcers, piles, cancer, gout, scrofula and act as abortifacient, anthelmintic, antipyretic and antiinflammatory drug. The main aim of the proposed work is to evaluate the anti-inflammatory activity of G. superba and also conserve the same plant through the in vitro propagation. The anti-inflammatory activity was evaluated by cycloxygenase inhibition assay and 5-lipoxygenase inhibition assay. In the cycloxygenase inhibition assay, the percentage inhibition were found to be 3.38%, 26.27%, 43.22% for sample concentration 100µg/ml, 500µg/ml, 1000µg/ml respectively and in the lipoxygenase inhibition assay percentage inhibition were found to be 49.23%, 76.92%, 84.61% for sample concentration 100µg/ml, 500µg/ml, 1000µg/ml respectively. In lipoxygenase inhibition assay the methanol extract of G. superba tuber showed close percentage inhibition with that of the standard aspirin. The present study thus confirmed that methanol extract of root tubers of G. superba possessed good anti-inflammatory activity. At present, the plant is on the way of extinction due to its misuse, over exploitation and unscientific collection. Micro propagation is an important method to conserve this highly antiinflammatory medicinal plant. In vitro studies of G. superba includes induction of callusing and organogenesis, using various explants. The results indicated that medium supplemented (MS) with auxin (NAA) (0.15 mg/l) and Benzylamino purine (BAP) (0.25 mg/l) induced callusing, with 2,4-D (0.5 mg/l) and Kinetin (0.25 mg/l) induced somatic embryogenesis, NAA (0. 5mg/l) and BAP (0.25 mg/l) promoted the formation of the maximum number of shooting and with NAA (0.25 mg/l) and BAP (0.15 mg/l) rooting was induced. Micro propagation will be helpful for the conservation and maximum utilization of this plant with high antiinflamatory potential along with the identification and isolation of useful bioactive molecules.

Antioxidant and Lipoxygenase Inhibitory Activities of Essential Oils from Endemic Plants of Côte d’Ivoire: Zanthoxylum mezoneurispinosum Ake Assi and Zanthoxylum psammophilum Ake Assi

“…The reaction was then initiated by addition of 35 µL of a substrate solution (linoleic acid 250 µM) and the absorbance was measured at 234 nm. Quercetin (Sigma) was used as a standard inhibitor at the same concentration as the essential oils [54,55,56]. The inhibition percentage of lipoxygenase activity was calculated as follows:Inhibition percentage (%) = 100 × (OD blank − OD sample )/OD blank where OD blank is the Optical Density (OD) of the reaction media without the essential oil, and OD sample is the OD of the reaction media with the essential oil minus the OD value of the diluted essential oil (to compensate for absorbance due to the essential oils themselves).…”

Analyzing the Bioactive Properties and Volatile Profiles Characteristics of Opuntia dillenii (Ker Gawl.) Haw: Exploring its Potential for Pharmacological Applications

“…The evaluation of the anti-inflammatory attributes in the extracts adhered to the protocol elucidated by Nikhila et al [42] In this methodology, a reaction concoction was formulated, encompassing 200 μL of the extract at diverse concentrations (100, 75, 50, and 25 μg/mL in methanol), 35 μL of lipoxygenase (Sigma, Darmstadt, Germany) at a concentration of 0.1 mg/mL, and 600 μL of a 0.2 M borate buffer solution with a pH of 9.0. Subsequently, this amalgamation was subjected to an incubation period of 15 minutes at a temperature of 25 °C.…”

Seasonal Effect on the Chemical Composition, Insecticidal Properties and Other Biological Activities of Zanthoxylum leprieurii Guill. & Perr. Essential Oils

“…The anti-inflammatory activities of Z. leprieurii essential oils were determined by the method previously described by Nikhila [30]. In brief, the reaction mixture containing essential oils in various concentrations (100, 75, 50 and 25 µg/mL of methanol) (in triplicate for each concentration), lipoxygenase (Sigma, Darmstadt, Germany) and 35 µL (0.1 mg/mL) of a 0.2 M borate buffer solution (pH = 9.0) was incubated for 15 min at 25 • C. The reaction was then initiated by the addition of 35 µL of a substrate solution (linoleic acid 250 µM), and the absorbance was measured at 234 nm.…”