Isolating RNA from clinical samples with catrimox-14 and lithium chloride

Macfarlane, Donald E.; Dahle, Christopher E.

doi:10.1002/(sici)1098-2825(1997)11:3<132::aid-jcla3>3.0.co;2-c

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…These kits, which are relatively expensive, can be very useful for isolating RNA enriched for specific sizes, since the kits vary greatly in the chemistry and/or mechanics used to lyse cells, denature and remove proteins and to actually isolate the RNA. The use of detergents to promote cell lysis led to the discovery of a cationic detergent (Catrimox-14®, Iowa Biotechnology Corp., Coralville, IA, USA) that both aided cell lysis and captured RNA and DNA by precipitation ( 5 , 6 ). This method had the major advantage of not using phenol and provided good yields of high-quality RNA ( 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

RNA snap ™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

Stead

Agrawal

Bowden

et al. 2012

Nucleic Acids Research

170

153

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RNAsnap™ is a simple and novel method that recovers all intracellular RNA quantitatively (>99%), faster (<15 min) and less expensively (∼3 cents/sample) than any of the currently available RNA isolation methods. In fact, none of the bacterial RNA isolation methods, including the commercial kits, are effective in recovering all species of intracellular RNAs (76–5700 nt) with equal efficiency, which can lead to biased results in genome-wide studies involving microarray or RNAseq analysis. The RNAsnap™ procedure yields ∼60 µg of RNA from 108 Escherichia coli cells that can be used directly for northern analysis without any further purification. Based on a comparative analysis of specific transcripts ranging in size from 76 to 5700 nt, the RNAsnap™ method provided the most accurate measure of the relative amounts of the various intracellular RNAs. Furthermore, the RNAsnap™ RNA was successfully used in enzymatic reactions such as RNA ligation, reverse transcription, primer extension and reverse transcriptase–polymerase chain reaction, following sodium acetate/ethanol precipitation. The RNAsnap™ method can be used to isolate RNA from a wide range of Gram-negative and Gram-positive bacteria as well as yeast.

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Section: Introductionmentioning

confidence: 99%

RNA snap ™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

Stead

Agrawal

Bowden

et al. 2012

Nucleic Acids Research

170

153

View full text Add to dashboard Cite

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] There has been considerable interest in characterizing the nature of the fundamental interactions between cationic agents and DNA, and several general approaches have been designed to unravel such interactions. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] One of the important driving forces in all of these systems is the long-range electrostatic interaction between molecules of opposite charge.…”

Section: Introductionmentioning

confidence: 99%

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Dasgupta¹,

Das²,

Dias³

et al. 2007

J. Phys. Chem. B

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The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the more hydrophilic surfactants. This was discussed in terms of surfactant packing. Observations are interpreted to reflect that the surfactants with more substituents have a larger headgroup and therefore form smaller micellar aggregates; these higher curvature aggregates lead to a less efficient, "patch-like" coverage of DNA. The more hydrophilic surfactants also presented a significantly lower cytotoxicity, which is important for biotechnological applications.

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“…[6][7][8][9] Cationic surfactant induced DNA and RNA precipitation has also been employed for plasmid DNA purification and RNA extraction. [10][11][12][13][14][15][16] However, the molecular mechanism of the interaction between plasmid DNA and cationic surfactants is not well understood. For example, it is not clear whether the interaction is driven by adsorption of cationic surfactant monomers to the phosphate groups of DNA through static charge-charge attraction or by the interaction of multiply charged surfactant aggregates with DNA phosphates.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism and Thermodynamics Study of Plasmid DNA and Cationic Surfactants Interactions

Zhu¹,

Evans²

2006

Langmuir

114

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The molecular mechanism and thermodynamics of the interactions between plasmid DNA and cationic surfactants were investigated by isothermal titration calorimetry (ITC), dynamic light scattering, surface tension measurements, and UV spectroscopy. The cationic surfactants studied include benzyldimethyldodecylammonium chloride, benzyldimethyltetradecylammonium chloride, cetylpyridinium chloride, and cetyltrimethylammonium chloride. The results indicate a critical aggregation concentration (cac) of a surfactant: above the cac the surfactant forms aggregates with plasmid DNA; below the cac, however, there is no detectable interaction between DNA and surfactant. Surfactants with longer hydrocarbon chains have smaller cac, indicating that hydrophobic interaction plays a key role in DNA-surfactant complexation. Moreover, an increase in ionic strength (I) increases the cac but decreases the critical micellization concentration (cmc). These opposite effects lead to a critical ionic strength (I(c)) at which cac = cmc; when I < I(c), cac < cmc; when I > I(c), DNA does not form complexes with surfactant micelles. In the interaction DNA exhibits a pseudophase property as the cac is a constant over a wide range of DNA concentrations. ITC data showed that the reaction is solely driven by entropy because both deltaH(o) (approximately 2-6 kJ mol(-1)) and deltaS(o) (approximately 70-110 J K(-1) mol(-1)) have positive values. In the complex, the molar ratio of DNA phosphate to surfactant is in the range of 0.63-1.05. The reaction forms sub-micrometer-sized primary particles; those aggregate at high surfactant concentrations. Taken together, the results led to an inference that there is no interaction between surfactant monomers and DNA molecules and demonstrated that DNA-cationic surfactant interactions are mediated by the hydrophobic interactions of surfactant molecules and counterion binding of DNA phosphates to the cationic surfactant aggregates.

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Isolating RNA from clinical samples with catrimox-14 and lithium chloride

Cited by 9 publications

References 14 publications

RNA snap ™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

RNA snap ™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Molecular Mechanism and Thermodynamics Study of Plasmid DNA and Cationic Surfactants Interactions

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