Isolated Vegetal Cortex from Xenopus Oocytes Selectively Retains Localized mRNAs

Elinson, Richard P.; King, Mary Lou; Forristall, Caryl

doi:10.1006/dbio.1993.1329

Cited by 64 publications

(41 citation statements)

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“…Mowry, unpublished results). Asymmetric distributions of mitochondria have been observed during oogenesis and early embryonic development in several organisms (Mignotte et al, 1987;Bement and Capco, 1990;Elinson et al, 1993;Calarco, 1995;Yost et al, 1995;Bavister and Squirrell, 2000;Motta et al, 2000). Despite the general uniformity of mitochondria, their size, structure, and/or number may be modified to meet changing requirements (reviewed in Bereiter-Hahn and Voth, 1994;Lloreta-Trull and Serrano, 1998).…”

Section: Discussionmentioning

confidence: 99%

Apparent mitochondrial asymmetry in Xenopus eggs

Володина

Denegre

Mowry

2003

Developmental Dynamics

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Section: Discussionmentioning

confidence: 99%

Apparent mitochondrial asymmetry in Xenopus eggs

Володина

Denegre

Mowry

2003

Developmental Dynamics

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“…Also, Kinesin II has been proposed to be involved in localization, since inhibiting Kinesin II activity in oocytes blocked vegetal localization (Betley et al 2004). Moreover, data suggest that localized mRNA is anchored at the vegetal pole by a mechanism that involves the cytokeratin filament network (Pondel and King 1988;Elinson et al 1993;Alarcon and Elinson 2001;Bubunenko and King 2001), and some surprising recent data suggest that vegetally localized mRNAs may actually play a structural role in the network (Kloc et al 2005).…”

Section: Introductionmentioning

confidence: 99%

40LoVe interacts with Vg1RBP/Vera and hnRNP I in binding the Vg1-Localization Element

Czaplinski¹,

Mattaj²

2005

RNA

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Localizing mRNAs within the cytoplasm gives cells the ability to spatially restrict protein production, a powerful means to regulate gene expression. Localized mRNA is often visible in microscopically observable particles or granules, and the association of mRNA localization with these structures is an indication that particles or granules may be essential to the localization process. Understanding how such structures form will therefore be important for understanding the function of localization RNPs (L-RNPs). We previously identified a novel component of an L-RNP from the Vg1 mRNA from Xenopus oocytes called 40LoVe. 40LoVe interaction with the Vg1-localization element (Vg1LE) was previously shown to be dependent on the VM1 and E2 sequence motifs within the Vg1LE that cross-link to hnRNP I and Vg1RBP/Vera, respectively. We report interaction of these motif-binding proteins with 40LoVe and identify a 40LoVe-Xenopus hnRNP D/AUF1 interaction. We further demonstrate that titration of VM1 and E2 motif binding activity in vivo surprisingly suggests that the motif binding proteins have differing roles during Vg1LE-dependent mRNA localization.

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“…Localized RNAs co-fractionate and co-immunoprecipitate with cytoskeletal elements that serve in both RNA transport and anchoring (Alarcon and Elinson, 2001;Boccaccio et al, 1999;Elinson et al, 1993;Forristall et al, 1995;Jankovics et al, 2002;Klymkowsky et al, 1991;Marikawa et al, 1997;Pondel and King, 1988;Shan et al, 2003;Stebbings, 2001;Yisraeli et al, 1990;Zhao et al, 1991). Interestingly, a noncoding RNA, Xlsirts (Kloc and Etkin, 1994) and VegT mRNA Zhang and King, 1996), when destroyed with antisense oligonucleotides (ODNs), caused release of specific RNAs from the vegetal cortex.…”

Section: Introductionmentioning

confidence: 99%