Isolasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Pirdot (Saurauia vulcani. Kurth)

Abstract

Pirdot plant(Saurauia vulcani. Kurth)is a plant that grows naturally in a tropical climate. During this decoction pirdot leaves plants are used by the people to treat diabetes disease. The purpose of this study was to isolate triterpenoid compound from ethyl acetate extract of pirdot leaves. The sample extraction was performed by total of maceration method using ethyl acetate as a solvent. The separation and purification of the compound was carried out by column chromatography method followed by stain pattern analysis with thin layer chromatography (TLC). Structure characterization of pure compound was determined using UV, IR, 1H-NMR, 13C-NMR, DEPT 135º, HMQC, HMBC, 1H-1H COSY and MS spectroscopies including compared with various literatures. Based on the analysis result, the pure isolate obtained was identified as a pentacyclic triterpenoid compound, 3-hydroxy acid, 12(13)-en,28-oleanolate acid with the molecular formula C30H48O3. The triterpenoid compound of pentacyclic oleanolic acid is the first reported from the pirdot plant (Saurauia vulcani. Kurth).

Triterpenoid compound from metanol extract of mangrove leaves (Sonneratia alba) and anti-cholesterol activity test

“…The IR spectrum (KBr) of isolated showed characteristic absorption frequencies at 3590 and 1236 cm -1 . This spectrum indicated of the O-H and C-O bond vibrations; the absorption observed at 897cm -1 was due to an unsaturated out of plane C-H vibration (Souza et al 2011;Situmeang et al 2018). The C=C vibrations was shown around 1687cm -1 as weakly intense band; stretching and bending vibrations due to methyl groups were represented by the bands at 2935cm -1 and 1462cm -1 and the signal at 1385cm -1 was due to methylenic vibration .…”

Potensi Nanoemulsi Ekstrak Daun Pirdot (Saurauia vulkani) dalam Meningkatkan Aktivasi Enzim Alpha-Amilase sebagai Alternatif Terapi Diabetes Mellitus

“…Penelitian Lumban, (2016) ekstrak etanol daun pirdot yang diekstraksi dengan cara maserasi memiliki aktivitas antidiabetes dan diperoleh bahwa daun pirdot mengandung bebeberapa metabolit sekunder seperti flavonoid, glikosida, steroid/triterpenoid, saponin dan tannin. Tumbuhan pirdot memiliki berbagai aktivitas di antaranya sebagai antibakteri, antitumor, antiinflamasi, hipoglikemik, antidiabetes, antihiperlipidemia dan antikoagulan [11]; [15]; [16]. Penelitian Simanjuntak et al, (2019) mengungkapkan aktivitas antidiabetes dari ekstrak etanol daun tumbuhan pirdot terhadap mencit yang diinduksi aloksan.…”

Senyawa Triterpenoid dari Ekstrak N-heksana Daun Kelor (Moringa oleifera Lamk.) dan Uji Aktivitas Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli

“…Spektrum IR isolat pada Gambar 2 (KBr) menunjukkan serapan gugus O-H (3431,0 cm -1 ) diikuti dengan serapan pada bilangan gelombang 1061,2 cm -1 yang merupakan regangan ulur dari gugus C-OH. Pada bilangan gelombang 2977,17 cm -1 terdapat regangan ulur gugus C-H alifatik diikuti dengan serapan pada 1458,70 cm -1 yang merupakan tekukan C-H pada 1370,33 cm -1 yang merupakan regang geminal dimetil (Martins dkk., 2013;Situmeang , 2018). Spekrum 1 H-NMR (Gambar 3) menunjukkan resonansi untuk proton olefinik pada pergeseran δ 4,68 dan 4,56 ppm (2H).…”