Isolasi Protein Dan Produksi Konsentrat Protein Daun (Kpd) Sebagai Suplemen Pakan Ternak

Yatno, Yatno; Suparjo, Suparjo; Murni, Rasmi

doi:10.24843/pastura.2018.v07.i02.p07

Cited by 2 publications

(3 citation statements)

References 2 publications

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“…Similar results were reported in previous studies that Leucaena was extracted twice using NaOH at pH 9 and 10, then continued with acetate buffer to produce LPC Leucaena products containing CP 54.82% and 52.60%, which was higher than extraction using NaOH at pH 8 of 46.98% [15]. This increase was very significant based on the protein content of lamtoro leaves of 28.2% [15]. The difference in the results obtained from this study compared to previous studies is caused by differences in extraction method, including pH and type of solution used, which will produce LPC with different CP content.…”

Section: Production and Amino Acid Composition Of Lpc I Zollingerianasupporting

confidence: 89%

“…The crude protein content of LPC I zollingeriana increased by 82.56% (from 29.35 to 53.58%). Similar results were reported in previous studies that Leucaena was extracted twice using NaOH at pH 9 and 10, then continued with acetate buffer to produce LPC Leucaena products containing CP 54.82% and 52.60%, which was higher than extraction using NaOH at pH 8 of 46.98% [15]. This increase was very significant based on the protein content of lamtoro leaves of 28.2% [15].…”

Section: Production and Amino Acid Composition Of Lpc I Zollingerianasupporting

confidence: 88%

“…LPC is produced through an extraction process to reduce non-protein components so that the product has a higher protein content than the original feed ingredient. LPC containing high CP resulting from the extraction of Leucena sp leaves using NaOH at pH 9 and 10 was 54.82 and 52.60%, respectively, and extraction using NaOH at pH 8 had a protein content of 46.98% [15]. Meanwhile, Leucaena sp leaves contain 22.03% protein [16].…”

Section: Introductionmentioning

confidence: 99%

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Leaf protein concentrate of Indigofera zollingeriana as a source of protein and branched-chain amino acids for ruminants

Puastuti,

Tresia,

Herliatika

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Indigofera zollingeriana (I zollingeriana) leaves contain high-level protein and amino acids. Thus, it can be used as a leaf protein concentrate (LPC) source. This study aimed to obtain LPC from I. zollingeriana as feed supplement for ruminants. LPC was prepared by extracting I. zollingeriana leaves using distilled water and 0.1 N NaOH with a ratio of 1:3. The extraction was done by adjusting the pH of the liquid extract at pH 2, 3, 3.5, 4, 4.5, 5, 5.5, 6, by adding 0.1N HCl. Each pH determination was repeated three times. The pH and precipitate values were regressed to determine the isoelectric pH, which indicated the highest amount of LPC obtained from the extraction. LPC was produced based on its isoelectric pH, and its composition was analyzed. The results showed that the isoelectric pH of I. zollingeriana leaf extraction using distilled water was higher at pH 4.9 compared to 0.1N NaOH extraction, which had a pH of 4.3. Additionally, the total precipitate obtained from the distilled water extraction (0.2044 g/ml) was greater than that obtained from the NaOH extraction (0.1870 g/ml). I. zollingeriana LPC extracted with distilled water produced LPC’s yield of about 3.64% of fresh I. zollingeriana leaf and produced 53.58% of CP. I. zollingeriana LPC had complete amino acids with an increase in the branched-chain amino acids (BCAAs) valine, leucine, and isoleucine by an average of 91.5%. It can be concluded that Indigofera zollingeriana LPC contains higher protein content and BCAAs.

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Section: Production and Amino Acid Composition Of Lpc I Zollingerianasupporting

confidence: 89%

Section: Production and Amino Acid Composition Of Lpc I Zollingerianasupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Leaf protein concentrate of Indigofera zollingeriana as a source of protein and branched-chain amino acids for ruminants

Puastuti,

Tresia,

Herliatika

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Enhancing feed fermentation in rice straw basal diets using leaf protein concentrate as a novel ruminant supplement derived from Indigofera zollingeriana

Puastuti,

Yulistiani,

Haryati

et al. 2024

Vet World

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Background and Aim: Rice straw, a widely available agricultural byproduct globally, has significant potential as a basal diet for livestock. The major challenge lies in obtaining high-protein foliage that can be easily extracted using natural water rather than chemical solvents. This study aimed to assess the ability of distilled water to extract protein concentrate from Indigofera leaves (Indigofera zollingeriana Miq.) and to evaluate its effectiveness in enhancing rumen feed fermentation and digestibility in low-quality rice straw basal diets. Materials and Methods: The study was conducted in two experimental series. Experiment 1 was designed to explore the ability of distilled water to extract protein concentrate from fresh and dry Indigofera leaves by comparing it with the 0.1 N NaOH standard solvent. Experiment 2 focused on the in vitro digestibility of protein concentrates extracted from fresh Indigofera leaves based on optimal findings from experiment 1. Five treatments consisting of 0.5% and 1.0% protein concentrate and two extractants (distilled water and 0.1 N NaOH) were used to extract protein from Indigofera leaves. These extracts were then added to rice straw-based diets. Rice straw without supplements was used as a control. The treatments were arranged using a randomized complete design with five replicates. Results: The results of experiment 1 showed that distilled water was superior to 0.1 N NaOH for extracting protein concentrate from fresh Indigofera leaves, as revealed by higher dry matter, protein yield, total amino acids (AA), and total essential AA (EAA) production. For in vitro experiment 2, supplementation with distilled water-extracted protein concentrates successfully increased rumen fermentation and digestibility in rice straw basal diets, as indicated by higher gas production, total volatile fatty acid, and microbial protein levels compared with 0.1 N NaOH. Conclusion: Findings from this study confirm that Indigofera leaf protein concentrate offers a new alternative for enhancing rumen feed fermentation and the digestibility of low-quality rice straw diets. This study implies that it is an easy, cost-effective, and environmentally friendly approach, particularly beneficial for smallholders, to extract protein concentrate from fresh Indigofera leaves using distilled water and use it to enhance the quality of rice straw for ruminant feed. The limitation of this study is that the Indigofera supplement was established using in vitro digestibility under controlled laboratory conditions, which does not reflect real rumen conditions. Therefore, further studies using in vivo digestibility in ruminant animals are required to confirm the ability of the protein extracted from indigofera to enhance rumen feed fermentation in low-protein basal diets. Keywords: distilled water solvent, Indigofera, leaf protein concentrate, rice straw, rumen fermentation.

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Isolasi Protein Dan Produksi Konsentrat Protein Daun (Kpd) Sebagai Suplemen Pakan Ternak

Cited by 2 publications

References 2 publications

Leaf protein concentrate of Indigofera zollingeriana as a source of protein and branched-chain amino acids for ruminants

Leaf protein concentrate of Indigofera zollingeriana as a source of protein and branched-chain amino acids for ruminants

Enhancing feed fermentation in rice straw basal diets using leaf protein concentrate as a novel ruminant supplement derived from Indigofera zollingeriana

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