Isolasi, Identifikasi, Dan Uji Antibakteri Kitosan Cangkang Bekicot (Achatina fulica) Terhadap Staphylococcus aureus dari Penderita Ulkus Diabetikum

Abstract

AbstrakSalah satu bakteri penyebab ulkus diabetikum adalah Staphylococcus aureus. Pengunaan antibiotik yang tidak tepat menyebabkan munculnya S. aureus yang resisten terhadap antibiotik atau bertahan hidup karena adanya perubahan genetic. Bahan alam yang memiliki sifat sebagai antibakteri adalah kitosan cangkang bekicot.Penelitian ini bertujuan untuk melakukan isolasi dan uji antibakteri kitosan cangkang bekicot terhadap Staphylococcus aureus dari penderita ulkus diabetikum dan juga dilakukan uji sensitifitas S. aureus terhadap kitosan cangkang bekicot. Pada penelitian inidilakukan secara true eksperimental. S. aureus diisolasi dari penderita ulkus diabetikum dan diidentifikasi berdasarkan sifat biakan, pewarnaan Gram, uji biokimiawi dan uji gula-gula. Isolat selanjutnya diuji sensitifitasnya terhadap kitosan cangkang bekicot. Hasil penelitian menunjukkan bahwa isolasi bakteri yang tumbuh dan dapat memfermentasi plat mannitol salt agar, sel berbentuk bulat bergerombol, bersifat Gram +, memfermentasi maltosa dan laktosa, mengkoagulasi plasma kelinci dan bereaksi positif terhadap uji clumping faktor dan Voges Proskouer. Pada penelitian ini, hasil uji terhadap kitosan cangkang bekicot pada kosentrasi 300-700 ppm adalah sensitif. Penelitian ini dapat disimpulkan bahwa penderita ulkus diabetikum yang diidentifikasi adalah Staphylococcus aureus. Isolat S. aureus sebagian besar sensitif terhadap kitosan cangkang bekicot (Achatina fulica).Kata kunci:Staphylococcus aureus, Ulkus Diabetikum, Kitosan Cangkang Bekicot (Achatina fulica). AbstractOne of the bacteria that causes diabetic ulcers is Staphylococcus aureus. Inappropriate use of antibiotics results in the emergence of antibiotic resistant S. aureus or survival due to genetic changes. The natural material that has antibacterial properties is gastropod shell chitosan (Achatina fulica). This study aims to isolate and antibacterial test of gastropod shell chitosan against Staphylococcus aureus from diabetic ulcer. In this study, it was carried out correctly experimental S. aureuswas isolated from diabetic ulcer patients and identified based on culture properties, Gram staining, biochemical tests and confectionery tests. The isolates were then tested for sensitivity to snail shell chitosan. The results showed that the isolation of bacteria that grew and could ferment the mannitol salt agar plate, clustered round cells, Gram +, ferment maltose and lactose, coagulase rabbit plasma and react positively to the clumping factor test and Voges Proskouer. In this study, the test results on gastropod shell chitosan (Achatina fulica) at a concentration of 300-700 ppm were sensitive. This study concluded that patients with diabetic ulcers identified were Staphylococcus aureus. S. aureus isolates are mostly sensitive to gastropod shell chitosan (Achatina fulica). PENDAHULUANPenderita diabetes mellitus, hiperglikemia dapat meningkatkan risiko komplikasi mikrovaskular dan makrovaskular, sehingga dapat meningkatkan risiko komplikasi yang lebih jauh, seperti retinopati, penyakit kardiov...

Snail Shell Waste Threat to Sustainability and Circular Economy: Novel Application in Food Industries

“…Chitin compounds are converted into chitosan through a chemical process that in demineralization, deproteinization, decolorization, and deacetylation (Figure 3) [27,36] The results of the chitosan test obtained from these shell species against potentially dangerous bacterial strains showed that an inhibitory power of 34.33 mm against Staphylococcus aureus was observed at an optimal concentration of 500 ppm [38]. Additional test results demonstrating a sensitive response were obtained from patients with diabetic ulcers caused by the Staphylococcus aureus bacteria at concentrations between 300 and 700 ppm [39]. Achatina fulica species-derived snail shell chitosan was tested for its positive antibacterial activity on cotton fabric by observing how different soaking times affected Staphylococcus aureus growth activity.…”

EFFECTS OF NANO CHITOSAN MOUTH SPRAY ON THE EPITHELIAL THICKNESS IN THE TRAUMATIC ULCUS HEALING PROCESS (In vivo)

“…3 Pemanfaatan sumber biomaterial bahan alam juga lebih dapat diterima oleh tubuh dibandingkan dengan bahan sintesis. 7 Bahan alami juga mempunyai keunggulan dibandingkan dengan bahan kimia karena ketersediaan bahan alami yang banyak. Aktivasi makrofag akan merangsang makrofag melepaskan IL-6 dan TNF-α yang dapat membunuh bakteri dan debris.…”