Isolasi dan Uji Aktivitas Antioksidan Ekstrak Metanol Dari Buah Senduduk Bulu (Clidemia hirta (L.) D. Don)

Abstract

A study on isolation and antioxidant activity of secondary metabolit of methanol extract of fruit of Senduduk Bulu (Clidemia hirta (L.) D. Don) has been carried out. Senduduk bulu is used for folk medicine to many diseases. Flavonoid and phenolic has been reported to its activity as antioxidant. The aim of this research was to find antioxidant compound and antioxidant activity of this fruit. Isolation works were conducted using Vacuum Liquid Chromatography and Column Chromatography method. Antioxidant activity was determined using DPPH free radical scavenging methods. The isolation works afforded 10 mg of pure compound CHP1 which was obtained as white crystal, with its melting point of 165–167ºC, soluble in chloroform and slightly soluble in methanol. This compound gave positive reaction with Liebermann-Burchard (LB) reagent and concluded as terpenoid. Based on it UV spectrum, this compound showed a λ max of 316 nm. Its FT-IR spectrum showed appearence of some functional groups of OH, C-H aliphatic, C=O ester,C=C alkene, CH bending and C-O. The 1H-NMR spectrum showed the alkene protons and methyl protons which is a characteristic of terpenoid compounds. The pure compound (CHP1) provided a weak antioxidant activity with the IC50 of 327,01µg/mL.

Antioxidant compounds and activity from the leaf of the mistletoe (Dendrophthoe pentandra (L.) Miq.) on Duku plant (Lansium domesticum Corr.)

“…The compound NH.1 was classified as a terpenoid, had an IC50 value of 151.35 μg/mL, and was categorized as a weak antioxidant. The IC50 value of the compound NH.1 from the parasite's leaves of duku is stronger than the IC50 value of the terpenoid compound from the fruit of Clidemia hirta (L.) D.Don with a value of 327.01 μg/mL (Fadhli et al, 2020). According to (Setiawan & Amalia, 2017), terpenoids work as antioxidants by donating hydrogen atoms so that they inhibit the occurrence of lipid peroxidation or LPO (damage to lipids in cell membranes by free radicals), which have the potential as free radicals.…”

“…However, when compared to the terpenoid compound from the fruit of Clidemia hirta (L.) D.Do, which is 327.01 g/mL. (Fadhli et al, 2020), The duku mistletoe shows more significant antioxidant activity. (Setiawan & Amalia, 2017) investigated that terpenoids functioned as antioxidants by donating hydrogen atoms, inhibiting the incidence of lipid peroxidation (LPO), which is damage to lipids in cell membranes caused by free radicals.…”

KAJIAN FITOKIMIA PIGMEN WARNA UNGU DAN PROFIL ANTIOKSIDAN PADA EKSTRAK BUNGA HARENDONG (Melastoma malabatrichum L.)

“…Pada bercak kelima dengan nilai Rf 0,83 menghasilkan bercak warna ungu termasuk kedalam golongan flavonoid. Sejalan dengan penelitian (Fidrianny et al, 2018;Fadhli et al, 2020;Septiani, 2021) bahwa senyawa-senyawa golongan metabolit sekunder yang berkontribusi dalam memberikan aktivitas antioksidan berasal dari senyawa golongan flavonoid dan fenolik. Dimana senyawa-senyawa golongan tersebut memiliki kemampuan untuk menyumbangkan atom hidrogennya kepada radikal bebas.…”