Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407

Abstract

<p>Isolation and Characterization of Actinomycetes Producing<br />Antibacterial Compound into Enteropatogenik<br />Escherichia coli K1.1, Pseudomonas pseudomallei 02 05<br />and Listeria monocytogenes 5407. Dwi N. Susilowati,<br />Ratih D. Hastuti, and Erny Yuniarti. The resistance of<br />bacterial pathogens to some antibacterial agents and side<br />effects of the antibacterial usage demanded discovery of<br />new effective, safe, and active antibacterial compounds.<br />Some pathogenic bacteria, such as enteropathogen Escherichia<br />coli (EPEC) that cause diarrhoea on children and<br />infants, Pseudomonas pseudomallei that cause melioidosis<br />on human and animal, and Listeria monocytogenes that<br />cause listeriosis on newly born babies mortality and death of<br />pregnant woman. Actinomycetes is the largest bacterial<br />group that produce antibiotics. More than 10,000 antibacterial<br />compounds had been discovered, two-third of<br />them were produced by this bacterial group. A study was<br />done to isolate and characterize Actinomycetes producing<br />antibacterial compounds effective against EPEC K1.1 and P.<br />pseudomallei 02 05. Soil samples were taken from 39<br />locations in Indonesia and 115 actinomycetes isolates were<br />obtained. Two of the isolates, i.e., isolate A3.5 that was<br />effective against P. pseudomallei 02 05 and isolate F6.1 that<br />was effective against EPEC K1.1 evaluated further. The<br />isolate A3.5 had an optimum time 72 hours to produce antibacterial<br />compound, while F6.1 took 96 hours. The antibacterial<br />compounds produced by both isolates were dissolve<br />in the a 70% ethyl acetate solution, but not in a 40oC<br />warm methanol solution because it is very dissolved. The<br />antibacterial compound extracted from the isolate A3.5 had<br />a similar effectiveness to antibiotics bacithracyn 10 unit and<br />neomycin 30 g. On the other hand, the antibacterial<br />compound extracted from isolate F6.1 had a similar effectiveness<br />to antibiotics colistin 10 g and doxyciclin 30 g.<br />Further identification of the isolates suggested that both of<br />them belongs to the genera Streptomyces.</p>

SKRINING DAN UJI DAYA HAMBAT ACTINOMYCETES DARI RIZOSFER TANAMAN KOPI ARABIKA DI KABUPATEN BENER MERIAH TERHADAP Collecotrichum coffeanum

“…Actinomycetes yang telah diisolasi dilakukan pemurninan dari setiap koloni yang berbeda berdasarkan pengamatan makroskopis dengan melihat warna dan bentuk koloni (Susilowati et al, 2007). Koloni Actinomycetes tersebut diambil menggunakan jarum ose kemudian dipindahkan ke media SCA yang telah padat dengan metode goresan kuadran (steak quadrant) untuk mendapatkan isolat murni (Sembiring et al, 2000).…”

Isolation of actinomycetes from peatland to suppress the growth of Ganoderma boninense the causal agent of basal stem rot disease in oil palm

“…Preparation of bioactive compounds was carried out by growing actinomycetes on liquid ISP2 medium and incubating on a shaker at 114 rpm for 7 days. The supernatant of actinomycetes cultures was separated and sterilized by 0.22 m millipore filter to obtain sterile crude bioactive compounds (Susilowati et al 2007). ISP medium was added with actinomycetes bioactive compounds.…”

ISOLASI Actinomycetes ENDOFIT DARI TANAMAN AKAR WANGI (Vetiveria zizanioides) DAN UJI AKTIVITAS SENYAWA ANTIBAKTERI TERHADAP Staphylococcus aureus DAN Escherichia coli

“…Pemurnian Actinomycetes endofit dilakukan pada setiap koloni yang dianggap berbeda berdasarkan morfologi makroskopis yang dapat dilihat dari penampakan warna meliputi hifa aerial, hifa substrat dan ada tidaknya pigmen yang terdifusi ke medium (pigmen terlarut) dan memproduksi spora seperti serbuk (Susilowati, 2007).…”

“…Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi (Susilowati et al, 2007). Media NA diusap sampai merata dengan suspensi bakteri target yang kekeruhannya setara dengan 0,5 Mc.Farland I menggunakan cotton swab, kemudian kertas saring berdiameter 6 mm steril dicelupkan selama 5 detik ke dalam suspensi isolat Actinomycetes endofit menggunakan pinset steril.…”