ISOLASI DAN IDENTIFIKASI SENYAWA FLAVONOID DARI BATANG CIPLUKAN (Physalis angulata L.)

Abstract

ABSTRAK
 Pemanfaatan tumbuhan di Indonesia banyak digunakan sebagai obat-obatan herbal dan sebagai upaya mempertahankan kesehatan masyarakat. Dari penelitian yang telah dilakukan, baik secara in vitro maupun in vivo, didapatkan informasi bahwa Ciplukan memiliki aktivitas sebagai antihiperglikemi, antibakteri, antivirus, imunostimulan dan imunosupresan (imunomodulator), antiinflamasi, antioksidan, dan sitotoksik. Selain itu dari berbagai laporan tumbuhan ini memiliki banyak khasiat tidak lain karena memiliki kandungan senyawa kimia yang fungsinya dapat mengobati suatu penyakit, salah satu senyawa kimia yang terkandung dalam tanaman Ciplukan ini adalah senyawa flavonoid. Berdasarkan dari hal ini maka dilakukanlah penelitian dengan isolasi dan identifikasi senyawa flavonoid yang terkandung dalam batang tanaman Ciplukan, Physalis angulata L. Metode yang digunakan adalah Uji Fitokomia, Kromatograpi Lapis Tipis, Kromatografi Kolom, dan Spektrofotometri UV-Vis. Hasil uji fitokimia menunjukan bahwa batang Ciplukan mengandung senyawa Kuinon, flavonoid, saponin, dan monosesquiterpeoid, dan polifenol. Uji KLT dengan eluen n-heksan-etil asetat (7:3) menunjukan kromatogram berwarna biru dengan Rf 0.8 cm. Beberapa fraksi hasil Kromatografi Kolom kemudian di Spektrofotometri UV-Vis menunjukan senyawa tersebut merupakan senyawa flavonoid jenis flavanon.
 Kata Kunci : Senyawa Flavonoid, Ciplukan (Physalis angulata L.).
 
 ABSTRACT
 The use of plants in Indonesia is widely used as herbal medicines and as an effort to maintain public health. One type of plant that is used as herbal medicines is the Ciplukan plant. In addition, from various reports of this plant has many other properties because it contains chemical compounds whose function is to treat a disease, one of the chemical compounds contained in this Ciplukan plant is a flavonoid compound. Based on this, a study was conducted with the isolation and identification of flavonoid compounds contained in the stem of the Ciplukan plant, Physalis angulata L. The methods used were Phytochomia Test, Thin Layer Chromatography, Column Chromatography, and UV-Vis Spectrophotometry. Phytochemical test results show that the Ciplukan stem contains Kuinon, flavonoid, saponin, and monosesquiterpeoid compounds, and polyphenols. TLC test with n-hexane-ethyl acetate (7: 3) eluent showed a blue chromatogram with Rf 0.8 cm. Several fractions of the results of the chromatography column later on UV-Vis Spectrophotometry showed that the compound was a flavonoid type of flavanone.
 Keynote : Flavonoid, Ciplukan (Physalis angulata L.).

Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine

“…Catechin compounds produced the feeling of drinking barrel tea; the compound was in the form of tannin, which did not correspond to the nature of the premise and smolded the protein, resulting in a bitter taste [20]. The scent of Baper tea was judged to be as huge as it was fragrant, indicating that the gallate acid was dried on the raw material of oxidized tea, resulting in the production of thearaubigin (TR) compound, which was responsible for the fragrant aroma generated [8]. Color analysis reveals that the color of the seduction generated by Baper tea is brilliant brown and yellowish-red brown, indicating the presence of a combination of filantin, quercetin, and theaflavin that alters the color of the seduction produced [21].…”

“…Chemical substances that regulate the immune system's function and activity to defend the body against viruses, infections and other external dangers are called immunomodulators. Each kind is classified as an immunostimulator (which promotes immune system activity), immunoregulator (which regulates immune system function), or immunosuppressor (which suppresses immune system function) [8]. Previous research has shown that plant compounds function as immunostimulators, overcoming immunodeficiency and improving immune system performance [9,10].…”

Antibacterial activity of betel (Piper betle L.) fruit against Pseudomonas aeruginosa

“…The isolate was centrifuged, then read on a UV-Vis spectrophotometer using methanol as a standard solvent. The spectrum is observed at a wavelength of 200-400 nm [6].…”

Formulasi dan Uji Stabilitas Fisik Sediaan Bedak Tabur Ekstrak Etanol Daun Ciplukan (Physalis angulata L.) Sebagai Anti Fungi di Desa Tammatto Kabupaten Bulukumba

“…(Wijanarko et al, 2020). Selain itu, Dari penelitian yang telah dilakukan, baik secara in vitro maupun in vivo, didapatkan informasi bahwa Ciplukan memiliki aktivitas sebagai antihiperglikemi, antibakteri, antivirus, imunostimulan dan imunosupresan (imunomodulator), antiinflamasi, antioksidan, dan sitotoksik (Ridwanuloh & Syarif, 2019). Tumbuhan ini secara empiris digunakan sebagai obat penyakit antijamur, asam urat, bisul, borok, demam, diabetes, dan hipertensi.…”