Isolasi Actinomycetes Dari Tanah Kebun Sebagai Bahan Petunjuk Praktikum Mikrobiologi

Pujiati, Pujiati

doi:10.25273/florea.v1i2.390

Cited by 5 publications

(7 citation statements)

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“…Actinomycetes dapat dijumpai dan tumbuh pada tanah pekarangan serta perkebunan dengan karateristik humus, kering, lebih dingin, dan di sekitar akar tumbuhan (Pujiati, 2014 (Ceylan et.al;2008).…”

Section: Pendahuluanunclassified

Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

Ariandi

Bahar

Yusmaini

et al. 2021

JBT

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Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.

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Section: Pendahuluanunclassified

Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

Ariandi

Bahar

Yusmaini

et al. 2021

JBT

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“…The distribution of Streptomyces in water and soil depends on pressure, temperature, pH, humidity, salinity, soil texture and climate [19]. Meanwhile, according to Pujiati, Streptomyces is mostly found in drier, more barren and colder soils [20].…”

Section: Soil Physical and Chemical Propertiesmentioning

confidence: 99%

Determination of the Physical and Chemical Properties of the Complex Latosol Soil, Mediterranean, Alluvial and Its Inhibitory Effect on Dog Saliva Bacteria

Sjamsiah¹,

Sappewali²,

Jannah³

et al. 2019

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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This research is to determine the physical and chemical properties of LatosolComplex soil, Mediterranean and Alluvial. In addition, a resistor power test is conducted soil with liquid soap against dog saliva bacteria using a method of sumuran, is by measuring the diameter of the inhibitory zone. The result of this research is obtained Latosol Complex soil has a pH of 4-5, dry weight of 0.07%-0.09%, water content of 6%-7%, and N-total 0.08%-0.19%. Mediterranean soil has a pH of 4.8-5.0, dry weight of 0.06%-0.16%, water content 5.8%-6.6%, N-total 0.09%-0.2%. Alluvial land has pH 6.7-6.9, weight dry 0.03%-0.50%, water content 3.0%-3.9%, and N-total 0.6%-1.7%. Optimum inhibitory zone of wild dog saliva bacteria was obtained in succession the combination of 10% Latosol Complex soil with liquid soap is 2.4 cm. Mix 20% Mediterranean soil with liquid soap is 2.4 cm. Aluvial 60% with liquid soap is 2.5 cm. At depth of soil is 30 cm each.

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“…Terdapat 52 uji pendukung karakterisasi bakteri Actinomycetes terpilih. Uji-uji tersebut juga dilakukan pada penelitian sebelumnya [17][18][19][20][21][22].…”

Section: Metode Penelitianunclassified

“…Nonmotil, indol negatif, ornithin negatif, tergolong aerob, katalase positif, dapat mereduksi sitrat, alkaline pada uji TSIA [20]. Koloni berwarna krim [19]. Permukaan kasar dan melekat pada media.…”

Section: Metode Penelitianunclassified

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Karakterisasi Fenotip dan Indeks Similaritas Isolat Actinomycetes yang Memiliki Kemampuan Antibakteri terhadap Escherichia coli dan Staphylococcus aureus

Sulistyanto

Trimulyono²

2019

Biotropika

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Eksplorasi kandidat mikrobia penghasil antibiotik semakin meningkat sebagai solusi banyaknya bakteri resisten terhadap antibiotik yang telah ada. Pada penelitian sebelumnya telah didapatkan empat isolat Actinomycetes yaitu ACB34a, ACB44c, ACB54c dan ACB55c yang memiliki kemampuan antibakteri, sehingga diperlukan identifikasi jenis isolate sampai tingkat Genus. Tujuan penelitian ini adalah mengidentifikasi jenis isolat yang terpilih (ACB34a, ACB44c, ACB54c dan ACB55c) berdasarkan karakteristik fenotip, dan menentukan indeks similaritas antarisolatnya. Identifikasi isolat dilakukan menggunakan karakter fenotip yang meliputi morfologi, fisiologi, biokimia dan dilanjutkan pengolahan numerik. Analisis data secara deskriptif dan numerik menggunakan Clad97. Hasil penelitian menunjukkan dua isolat Actinomycetes (ACB34a dan ACB55c) memiliki kemiripan dengan Genus Actinomadura. Sedangkan dua isolate berikutnya, yaitu ACB44c dan ACB54c masing-masing mirip dengan Genus Nocardia dan Streptomyces. Nilai indeks similaritas antara isolat ACB34a dan ACB55c adalah 0,856, selanjutnya isolat ACB54c dan ACB44c memiliki kesamaan 0,788, dan isolat ACB55c dan ACB34a memiliki kesamaan sebesar 0,7788.

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Isolasi Actinomycetes Dari Tanah Kebun Sebagai Bahan Petunjuk Praktikum Mikrobiologi

Cited by 5 publications

References 0 publications

Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

Determination of the Physical and Chemical Properties of the Complex Latosol Soil, Mediterranean, Alluvial and Its Inhibitory Effect on Dog Saliva Bacteria

Karakterisasi Fenotip dan Indeks Similaritas Isolat Actinomycetes yang Memiliki Kemampuan Antibakteri terhadap Escherichia coli dan Staphylococcus aureus

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